EasySep™ Direct Human Basophil Isolation Kit
Immunomagnetic negative selection from whole blood kit
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EasySep™ Direct Human Basophil Isolation Kit -
Label for EasySep™ Direct Human Basophil Isolation Kit
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Required Products
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Cell separation buffer -
"The Big Easy" EasySep™ Magnet
Magnet for column-free immunomagnetic separation -
Compatible antibodies for purity assessment of isolated cells
Overview
The EasySep™ Direct Human Basophil Isolation Kit isolates functional, highly purified basophils directly from human whole blood by immunomagnetic negative selection. No lysis, density gradient centrifugation, or other processing steps are required and isolated cells are immediately available for flow cytometry, functional assays, culture, and other downstream applications.
Advantages:
• Effectively depletes RBCs without the need for density gradient centrifugation, sedimentation, or lysis
• Up to 98% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
• Up to 98% purity of isolated cells
• Fast, easy-to-use and column-free
• Isolated cells are untouched
Components:
- EasySep™ Direct Human Basophil Isolation Kit (Catalog #19667)
- EasySep™ Direct Human Basophil Isolation Cocktail, 2 x 2.5 mL
- EasySep™ Direct RapidSpheres™ 50300, 4 x 2.5 mL
Magnet Compatibility:
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• Easy 50 EasySep™ Magnet (Catalog #18002)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
Subtype:
Cell Isolation Kits
Cell Type:
Granulocytes and Subsets
Species:
Human
Sample Source:
Whole Blood
Selection Method:
Negative
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
Related Products
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EasySep™ Direct Human Neutrophil Isolation Kit
Immunomagnetic negative selection from whole blood kit -
Magnet for column-free immunomagnetic separation -
EasySep™ Direct Human Pan-Granulocyte Isolation Kit
Immunomagnetic negative selection from whole blood kit
Labeling Antibodies
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Anti-Human CD45 Antibody, Clone HI30
Mouse monoclonal IgG1 antibody against human, chimpanzee CD45 -
Anti-Human CD123 (IL-3Rα) Antibody, Clone 6H6
Mouse monoclonal IgG1 antibody against human, rhesus, sooty mangabey CD123 (IL-3Rα)
Scientific Resources
Product Documentation
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(11)
Product Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Research Area
Workflow Stages for
Workflow Stages
Data and Publications
Data
Figure 1. Typical EasySep™ Direct Human Basophil Isolation Profile
Starting with human whole blood from normal healthy donors, the typical basophil (CD123+IgE+) content of the non-lysed final isolated fraction is 97.3 ± 1.1% (gated on CD45). In the above example, the basophil content of the lysed whole blood start sample and the non-lysed final isolated fraction is 0.73% and 96.0% (gated on CD45), respectively, or 0.68% and 89.8% (not gated on CD45), respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.