Doxycycline (Hyclate)

Antibiotic; Used in Tet-inducible gene expression systems

Doxycycline (Hyclate)

Antibiotic; Used in Tet-inducible gene expression systems

From: 398 USD
Catalog #
72742_C
Antibiotic; Used in Tet-inducible gene expression systems

Overview

Doxycycline is a tetracycline-like antibiotic (Hicks et al.; Nau et al.; Solís García del Pozo & Solera; Steinhardt et al.). In genetic engineering, doxycycline is used as the regulator for inducible gene expression systems, whereby expression depends on either the presence (Tet-On) or absence (Tet-Off ) of doxycycline. Also, doxycycline inhibits certain matrix metalloproteinases (MMP), such as MMP-8 (Ki = 36 µM; Griffin et al.; Smith et al.). It only poorly inhibits MMP-1 and MMP-13 (Ki > 100 µM; Smith et al.). This product is supplied as the hyclate salt of the molecule.

CELL LINE DEVELOPMENT
· Used as the regulator for inducible gene expression in lentiviral infection and transgenic mouse models using the Tet-On or Tet-Off systems (Brambrink et al.; Carey et al.; Haenebalcke et al.; Hanna et al.; Hockemeyer et al.; Maherali et al.; Markoulaki et al.; Stadtfeld et al. 2008, 2010; Wernig et al.).
Alternative Names
WC 2031
Cell Type
CHO Cells, Pluripotent Stem Cells
Species
Human, Mouse, Rat, Non-Human Primate, Other
Application
Antibiotic
Area of Interest
Cell Line Development
CAS Number
24390-14-5
Chemical Formula
2[C₂₂H₂₄N₂O₈] · 2HCl · H₂O · C₂H₆O
Molecular Weight
1025.9 g/mol
Purity
≥ 98%

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
72742
Lot #
All
Language
English
Catalog #
72742, 100-1047
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
72742
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-1047
Lot #
All
Language
English

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