Human Peripheral Blood CD4+CD25+CD127lowFOXP3+ T cells (Tregs), Frozen
Primary human cells, frozen
Overview
Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
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Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Data and Publications
Figure 1. Cryopreserved Tregs Suppress CD8+ T Cell Proliferation
Human Peripheral Blood Mononuclear Cells (PBMCs), Frozen (Catalog #70025) and PB CD4+CD25+CD127lowFoxP3+ T cells (Tregs), Frozen (Catalog #200-0120) were co-cultured for 4 days at various ratios of Treg:PBMCs in the presence of 2.5 μL/mL ImmunoCultTM Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981). The Treg-mediated suppression of CD8+ T cell proliferation ranged from >90% at Treg:PBMC ratios of 1:1 to <5% at Treg:PBMC ratios of 1:32. Experiments were performed with 3 independent Treg donors, represented by the gray, orange, and black lines (mean ± SD, n = 3).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
