Human Peripheral Blood CD4+CD25+CD127lowFOXP3+ T cells (Tregs), Frozen
Primary human cells, frozen
Overview
Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
Scientific Resources
Product Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Data and Publications

Figure 1. Cryopreserved Tregs Suppress CD8+ T Cell Proliferation
Human Peripheral Blood Mononuclear Cells (PBMCs), Frozen (Catalog #70025) and PB CD4+CD25+CD127lowFoxP3+ T cells (Tregs), Frozen (Catalog #200-0120) were co-cultured for 4 days at various ratios of Treg:PBMCs in the presence of 2.5 μL/mL ImmunoCultTM Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981). The Treg-mediated suppression of CD8+ T cell proliferation ranged from >90% at Treg:PBMC ratios of 1:1 to <5% at Treg:PBMC ratios of 1:32. Experiments were performed with 3 independent Treg donors, represented by the gray, orange, and black lines (mean ± SD, n = 3).
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.