Human Peripheral Blood CD4+CD25+CD127lowFOXP3+ T cells (Tregs), Frozen

Primary human cells, frozen

Human Peripheral Blood CD4+CD25+CD127lowFOXP3+ T cells (Tregs), Frozen

Primary human cells, frozen

From: 4,932 USD
Catalog #
200-0120_C
Primary human cells, frozen

Overview

Primary human CD4+CD25+CD127lowFOXP3+ T cells were isolated from peripheral blood using immunomagnetic separation techniques. These cells are often referred to as regulatory T cells and are used in immune response assays.

Cells were obtained using Institutional Review Board (IRB)-approved consent forms and protocols.
Cell Type
T Cells, Regulatory
Species
Human
Cell and Tissue Source
Peripheral Blood
Donor Status
Normal
Purity
≥ 85% CD3+CD4+CD25+CD127low and ≥ 80% CD3+CD4+CD25+FOXP3+ by flow cytometry

Scientific Resources

Product Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
200-0120, 200-0121
Lot #
All
Language
English

Data and Publications

Figure 1. Cryopreserved Tregs Suppress CD8+ T Cell Proliferation

Human Peripheral Blood Mononuclear Cells (PBMCs), Frozen (Catalog #70025) and PB CD4+CD25+CD127lowFoxP3+ T cells (Tregs), Frozen (Catalog #200-0120) were co-cultured for 4 days at various ratios of Treg:PBMCs in the presence of 2.5 μL/mL ImmunoCultTM Human CD3/CD28 T Cell Activator (Catalog #10971) and ImmunoCult™-XF T Cell Expansion Medium (Catalog #10981). The Treg-mediated suppression of CD8+ T cell proliferation ranged from >90% at Treg:PBMC ratios of 1:1 to <5% at Treg:PBMC ratios of 1:32. Experiments were performed with 3 independent Treg donors, represented by the gray, orange, and black lines (mean ± SD, n = 3).

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