STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing

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STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing

From: 662 USD
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For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing
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What's Included

  • STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
  • Nuclease-Free Water, 2 x 1.25 mL
  • STEMscript™ Reaction Buffer with Random Primers, 2 x 200 µL
Products for Your Protocol

Overview

Use STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers for reverse transcription of eukaryotic mRNA prior to qPCR or RNA sequencing.

This kit includes three components that have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification: STEMscript™ Reverse Transcriptase Mix (contains STEMscript™ Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), STEMscript™ Reaction Buffer (contains primers, MgCl₂, and dNTPs), and nuclease-free water.

Oligo(dT) primers are ideal for reverse transcription of eukaryotic mRNA, as they anneal to poly(A) tails. For reverse transcription of RNA without poly(A) tails (e.g. rRNA, tRNA, degraded RNA, RNA isolated from prokaryotic sources, and RNA with known secondary structures), we recommend using STEMscript™ cDNA Synthesis Kit with Random Primers.
Species
Human, Mouse, Rat
Brand
STEMscript

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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79003
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English
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Safety Data Sheet 1
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79003
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English
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Safety Data Sheet 2
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79003
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English
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Safety Data Sheet 3
Catalog #
79003
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All
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English

Resources and Publications

Educational Materials (1)

New look, same high quality and support! You may notice that your instrument or reagent packaging looks slightly different from images displayed on the website, or from previous orders. We are updating our look but rest assured, the products themselves and how you should use them have not changed. Learn more