Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit

For detection of human anti-SARS-CoV-2 nucleoprotein IgG antibodies
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit

For the detection of human anti-SARS-CoV-2 nucleoprotein IgG antibodies

1 Kit
Catalog # 100-0686
1,047 USD

Overview

The Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit is designed for the qualitative detection of human SARS-CoV-2 nucleoprotein (NP) IgG antibody in biological fluids such as serum and plasma.

Coronaviruses (CoV), including SARS-CoV-2, are enveloped and contain single-stranded (positive-sense) RNA associated with a nucleoprotein. These nucleoproteins are abundantly produced within CoV-infected cells, where their primary function is to package the viral genome into flexible, helical ribonucleoprotein complexes called nucleocapsids. The nucleocapsid binds to genomic RNA and is understood to interact with viral membrane proteins during virion assembly, therefore playing a critical role in ensuring the efficiency of viral transcription, translation, and assembly. In host cells, the nucleocapsid can directly impact normal cell functions and cause cell-cycle deregulation, increased COX-2 production, and inhibition of interferon production.

The assay is based on the indirect ELISA method, in which samples are added to ELISA strip plates pre-coated with human novel coronavirus nucleoprotein. Antibodies present in the sample specific for the coronavirus nucleoprotein will bind to the pre-coated antigen. The captured antibodies are detected by addition of an anti-human IgG-conjugated horseradish peroxidase (HRP). Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human novel coronavirus nucleoprotein IgG antibody in the sample.
Components
  • Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit(Catalog #100-0XXX)
    • Human SARS-CoV-2 NP IgG ELISA Plate, 1x
    • Human SARS-CoV-2 NP IgG Negative Control, 800 µL
    • Human SARS-CoV-2 NP IgG Positive Control, 800 µL
    • Human IgG HRP-Conjugated Detection Antibody, 120 µL
    • Human IgG HRP Antibody Diluent, 20 mL
    • ELISA Diluent, 2 x 20 mL
    • Wash Buffer (25X), 20 mL
    • TMB Substrate, 10 mL
    • Stop Solution, 10 mL
    • Adhesive Plate Covers, 4x
Subtype
Complete Kits
Reactive Species
Human
Application
ELISA
Area of Interest
Immunology, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 5
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 6
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 7
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 8
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English
Document Type
Safety Data Sheet 9
Product Name
Human SARS-CoV-2 Nucleoprotein IgG Antibody ELISA Kit
Catalog #
100-0686
Lot #
All
Language
English

Educational Materials (5)

Brochure
Tools For Your Immunology Research
Brochure
ELISA Kits for Immunology Research
Wallchart
SnapShot: COVID-19
Video
Collaborating to Accelerate COVID-19 Research
3:36
Collaborating to Accelerate COVID-19 Research
Webinar
SARS-CoV-2 Viral Diversity: Causes, Consequences, and Opportunities
45:33
SARS-CoV-2 Viral Diversity: Causes, Consequences, and Opportunities

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