The enzyme-linked immunosorbent assay (ELISA) is a quantitative, highly sensitive assay for the detection of analytes present in biological samples such as serum, plasma and cell culture supernatants. This widely used immunoassay can be used to evaluate cell activation and/or differentiation through the detection and accurate quantification of substances such as cytokines, hematological factors, hormones, peptides, and immunoglobulins.
Take the guesswork out of cell analysis and evaluate your cells using ELISA Kits that are compatible with your workflow. Choose from a selection of highly-sensitive ELISA kits with low intra- and inter-assay variability for accurate, precise, and consistent analyte quantification.
Complete, ready-to-use ELISA kits are conveniently packaged with all the required standards, substrates, and solutions — as well as pre-coated 96-well strip microplates — to reduce assay time and variability. Alternatively, ELISA antibody pair kits offer the flexibility to customize your assay and come with optimized and matched quantities of capture and detection antibodies, plus an analyte standard. In both types of ELISA kit, included standards are calibrated against international references for accurate results.
How It Works
The sandwich ELISA protocol involves immobilizing an antibody against the analyte of interest to a solid surface, typically a 96-well polystyrene microplate. A test sample is then added to the microplate and the target analyte, if present, binds to the immobilized antibody. Unbound proteins are then washed away, leaving only the analyte of interest. The bound target is then recognized and bound by a second analyte-specific antibody, which is linked (either directly or via Biotin-Streptavidin) to a detection enzyme. Detection and quantification of the target analyte is accomplished by adding a substrate for the linked enzyme and measuring the color change reaction. The most commonly used enzymes for ELISA are horseradish peroxidase (HRP) and alkaline phosphatase (AP).