Mouse/Rat Recombinant Noggin, ACF

Noggin, animal component-free

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Mouse/Rat Recombinant Noggin, ACF

Noggin, animal component-free

From: 939 USD

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100 µg

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Noggin, animal component-free

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Overview

Noggin binds to and antagonizes bone morphogenetic protein (BMP) ligands of the transforming growth factor beta (TGF-β) family. Noggin supports maintenance of undifferentiated human embryonic stem cells cultured in mouse embryonic fibroblast (MEF)-conditioned medium (Chaturvedi et al.), and promotes dopaminergic differentiation of embryonic stem cells and subsequent survival of dopamine neurons (Chiba et al.). Noggin is essential for development of ectodermal structures including neural tube, tooth, hair follicle, and eye, as well as patterning of mesodermal somites and skeletal structures. It also influences chondrogenesis, osteogenesis, and joint formation (Krause et al.). This product is animal component-free (ACF).

Cytokines

NOG, SYM1, SYNS1

Neural Stem and Progenitor Cells, Neurons, Pluripotent Stem Cells

Mouse, Rat

Neuroscience, Stem Cell Biology

Animal Component-Free

≥ 98% by SDS-PAGE

Data Figures

Figure 1. Biological Activity and Molecular Mass of Mouse/Rat Recombinant Noggin, ACF

(A) The biological activity of Mouse/Rat Recombinant Noggin, ACF was tested by its ability to inhibit BMP-2 activity in HEK293T cells using a luciferase reporter assay. Firefly luciferase activity was normalized to the control Renilla luciferase activity. The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 2.1 nM.

(B) 3 μg of Mouse/Rat Recombinant Noggin, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Mouse/Rat Recombinant Noggin, ACF has a predicted molecular mass of 46 kDa (dimer). Noggin has an unusual migration pattern under non-reducing conditions due to the non-covalent dimer which is the active protein.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type

Product Name

Catalog #

Lot #

Language

Document Type

Product Information Sheet

Product Name

Mouse/Rat Recombinant Noggin, ACF

Catalog #

100-2068

Lot #

All

Language

English

Document Type

Safety Data Sheet

Product Name

Mouse/Rat Recombinant Noggin, ACF

Catalog #

100-2068

Lot #

All

Language

English

Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

Mouse/Rat Recombinant Noggin, ACF

Noggin, animal component-free

Mouse/Rat Recombinant Noggin, ACF

Noggin, animal component-free

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Overview

Data Figures

Protocols and Documentation

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