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Human Recombinant Gremlin-1, ACF

Gremlin-1, animal component-free

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Human Recombinant Gremlin-1, ACF

Gremlin-1, animal component-free

From: 449 USD
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Gremlin-1, animal component-free
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Overview

Gremlin-1 is a secreted protein that specifically binds to bone morphogenic proteins (BMP) family proteins including BMP-2 and BMP-4, preventing them from interacting with their receptors (Hsu et al.). Gremlin-1 is expressed in colon basal crypts where it is thought to contribute to the maintenance of the stem cell niche (Kosinski et al.). Gremlin-1 expression is elevated in disease, including some colon tumors (Kosinski et al.), liver disease, and diabetes (Baboota et al.). Additionally, overexpression of Gremlin-1 has been shown to promote cancer cell migration, proliferation, and invasiveness in a VEGFR2- and BMP-independent manner (Kim et al.). Gremlin-1 has been used for the culture of human colon epithelia and carcinoma organoids (Urbischek et al.). This product is animal component-free (ACF).
Subtype
Cytokines
Alternative Names
BMP antagonist 1, Cell proliferation-inducing gene 2 protein, Cysteine knot superfamily 1, DAN domain family member 2, Down-regulated in Mos-transformed cells protein, DRM
Species
Human, Other
Area of Interest
Cancer, Epithelial Cell Biology
Formulation Category
Animal Component-Free
Purity
≥ 98% by SDS-PAGE

Data Figures

Biological Activity and Molecular Mass of Human Recombinant Gremlin-1, ACF

Figure 1. Biological Activity and Molecular Mass of Human Recombinant Gremlin-1, ACF

(A) The biological activity of Human Recombinant Gremlin-1, ACF was tested by its ability to inhibit BMP-2 activity in stably transfected HEK293T cells using a luciferase reporter assay. Firefly luciferase activity is normalized to the control Renilla luciferase activity.The EC50 is defined as the effective concentration of the growth factor at which inhibition of BMP-2 activity is at 50% of maximum. The EC50 in the above example is 1.88 nM (34 ng/mL).

(B) 7 μg of Human Recombinant Gremlin-1, ACF was resolved with SDS-PAGE under reducing (+) and non-reducing (-) conditions and visualized by Coomassie Blue staining. Human Recombinant Gremlin-1, ACF has a predicted molecular mass of 18 kDa (monomer). The characteristic diffused bands are due to the dissociation of the non-covalent dimer during electrophoresis.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
Human Recombinant Gremlin-1, ACF
Catalog #
100-2073
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
Human Recombinant Gremlin-1, ACF
Catalog #
100-2073
Lot #
All
Language
English
Quality Statement:

PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.

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