EasySep™ Release Human APC Positive Selection Kit
Immunomagnetic positive selection kit using particle release technology
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EasySep™ Release Human APC Positive Selection Kit -
Label for EasySep™ Release Human APC Positive Selection Kit
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Required Products
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Magnet for column-free immunomagnetic separation -
Cell separation buffer
Overview
The EasySep™ Release Human APC Positive Selection Kit is designed to isolate cells that are labeled with APC-conjugated antibodies from fresh or previously frozen peripheral blood mononuclear cells or washed leukapheresis samples by immunomagnetic positive selection.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Desired cells are labeled with antibodies and magnetic particles, and separated without columns using an EasySep™ magnet. Unwanted cells are simply poured off, while desired cells remain in the tube. Then, bound magnetic particles are removed from the EasySep™-isolated, APC-antibody labeled cells, which are immediately available for downstream applications. Following cell isolation with this EasySep™ Release kit, antibody complexes remain bound to the cell surface and may interact with Brilliant Violet™ antibody conjugates, polyethylene glycol-modified proteins or other chemically related ligands.
Advantages:
• Highly purified cells labeled with APC-conjugated antibodies isolated from human samples in less than 40 minutes
• No-wash removal of EasySep™ Releasable RapidSpheres™
• No-wash removal of EasySep™ Releasable RapidSpheres™
Components:
- EasySep™ Release Human APC Positive Selection Kit (Catalog #100-0031)
- EasySep™ Release APC Positive Selection Cocktail, 1 mL
- EasySep™ Releasable RapidSpheres™, 1 mL
- EasySep™ Release Buffer (Concentrate), 3 x 1 mL
- Anti-Human CD32 Blocker for Positive Selection, 1 mL
Magnet Compatibility:
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• EasyEights™ EasySep™ Magnet (Catalog #18103)
• EasyPlate™ EasySep™ Magnet (Catalog #18102)
Subtype:
Cell Isolation Kits
Cell Type:
Other
Species:
Human
Sample Source:
Leukapheresis; Other; PBMC
Selection Method:
Positive
Application:
Cell Isolation
Brand:
EasySep
Area of Interest:
Immunology
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Scientific Resources
Product Documentation
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Educational Materials
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Data and Publications
Data
Figure 1. Purity of CD45+ Cells Following Cell Isolation with the EasySep™ Release Human APC Positive Selection Kit
In the above example, the frequencies of CD45+ cells in the starting and isolated fractions are 5.9% and 94.2%, respectively.
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.