EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit

Immunomagnetic negative selection kit
EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit

2.5 x 10^8 cells
Catalog # 17965
432 USD
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Required Products
  1. EasySep™ Magnet
    EasySep™ Magnet

    Magnet for column-free immunomagnetic separation

  2. STEMdiff™ Cardiomyocyte Support Medium
    STEMdiff™ Cardiomyocyte Support Medium

    Medium for thawing and culturing hPSC-derived cardiomyocytes

Overview

The EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit is designed to isolate human PSC-derived cardiomyocytes from cell cultures by negative selection. Unwanted cells are targeted for removal with Tetrameric Antibody Complexes recognizing non-cardiomyocytes and dextran-coated magnetic particles. The labeled cells are separated using an EasySep™ magnet without the use of columns. Desired cells are poured off into a new tube.
Advantages
• Fast and easy-to-use
• Up to 99% purity
• Isolated cells are untouched
Components
    • EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Cocktail, 0.5 mL
    • EasySep™ Dextran RapidSpheres™ 50100, 1 mL
 
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
Subtype
Cell Isolation Kits
Species
Human
Sample Source
Other
Selection Method
Negative
Application
Cell Isolation
Brand
EasySep, STEMdiff
Area of Interest
Disease Modeling, Drug Discovery and Toxicity Testing

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit
Catalog #
17965
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit
Catalog #
17965
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
EasySep™ Human PSC-Derived Cardiomyocyte Enrichment Kit
Catalog #
17965
Lot #
All
Language
English

Educational Materials(5)

Brochure
STEMdiff™ Cardiomyocyte Differentiation Kit
Brochure
EasySep™ Cell Separation Technology
Video
1:13
Isolate Cells with a Simple Pour-Off: EasySep™ Cell Separation Technology
Webinar
48:54
Highly Efficient Single-Cell Human Pluripotent Stem Cell Cloning and Robust Cardiomyocyte Differentiation
Webinar
43:45
Modeling Arrhythmias Using hPSC-Derived Cardiomyocytes and Tracking Their Excitability

Product Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.

Research Area
Workflow Stages

Data and Publications

Data

Starting with 1C cells differentiated using STEMdiff™ Cardiomyocyte Differentiation Kit (Catalog #05010), the cTnT+ cell content of the enriched fraction is typically 92.5 ± 7.6% (mean ± SD using the purple EasySep™ Magnet). In the above example, the purities of the start and final enriched fractions are 80.2% and 96.0%, respectively.

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