Use STEMscript™ cDNA Synthesis Kit with Random Primers for reverse transcription of RNA without poly(A) tails (e.g. rRNA, tRNA, degraded RNA, RNA isolated from prokaryotic sources, and RNA with known secondary structures) prior to qPCR or RNA sequencing.

This kit includes three components that have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification: STEMscript™ Reverse Transcriptase Mix (contains STEMscript™ Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), STEMscript™ Reaction Buffer (contains primers, MgCl₂, and dNTPs), and nuclease-free water.

Random primers are ideal for reverse transcription of RNA without poly(A) tails. For reverse transcription of eukaryotic mRNA with poly(A) tails, please use STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers, which includes primers that anneal to the poly(A) tails