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LysoBrite™ Lysosome Dyes

Fluorescent dyes used to label and track lysosomes in live cells

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LysoBrite™ Lysosome Dyes

Fluorescent dyes used to label and track lysosomes in live cells

From: 320 USD
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Fluorescent dyes used to label and track lysosomes in live cells
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Overview

LysoBrite™ dyes are fluorescent dyes that selectively stain lysosomes and other acidic organelles. Due to their hydrophobic nature, LysoBrite™ dyes permeate live cells and selectively accumulate in the lysosomes via the lysosome’s pH gradient. The fluorescence property of these dyes significantly increases within the lysosome’s acidic environment; this fluorescence can be measured using flow cytometry and fluorescence imaging. Due to their selective fluorescence inside lysosomes, LysoBrite™ dyes provide low background and high contrast during imaging. They can be used with proliferating and non-proliferating cells, and are suitable for suspension and adherent cells.
Cell Type
Pluripotent Stem Cells
Application
Characterization
Area of Interest
Autophagy, Stem Cell Biology
CAS Number
67-68-5
Molecular Weight
LysoBrite™ Lysosome Dye, Deep Red*: 749.99 g/mol; LysoBrite™ Lysosome Dye, Red DND-99* : 399.25 g/mol; LysoBrite™ Lysosome Dye, Green*: 453.34 g/mol

Data Figures

Image of H9 embryonic stem cells stained with LysoBrite™ Lysosome Dye.

Figure 1. LysoBrite™ Lysosome Dyes Can Be Used to Stain Embryonic Stem Cells

H9 embryonic stem cells were plated in mTeSR™ Plus (Catalog #100-0276) on Corning® Matrigel®-coated 35 mm glass-bottom dishes for 24 hours, then stained with LysoBrite™ Lysosome Dye, Green (green), and counterstained with Hoechst 33342 (blue).

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Document Type
Product Information Sheet
Product Name
LysoBrite™ Lysosome Dye, Deep Red
Catalog #
100-0819
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
LysoBrite™ Lysosome Dye, Green
Catalog #
100-0851
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
LysoBrite™ Lysosome Dye, Red DND-99
Catalog #
100-0850
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
LysoBrite™ Lysosome Dye, Deep Red
Catalog #
100-0819
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
LysoBrite™ Lysosome Dye, Green
Catalog #
100-0851
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
LysoBrite™ Lysosome Dye, Red DND-99
Catalog #
100-0850
Lot #
All
Language
English

Resources and Publications

Educational Materials (3)

hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Brochure
hPSC Differentiation: Tools for Pluripotent Stem Cell-Derived Research
Products for Human Pluripotent Stem Cells
Brochure
Products for Human Pluripotent Stem Cells
Derivation and Applications of Human Pluripotent Stem Cells
Wallchart
Derivation and Applications of Human Pluripotent Stem Cells

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