CoV Protease Substrate-1 TF5

FRET-based peptide substrate for detection of protease activity of coronaviruses

CoV Protease Substrate-1 TF5

FRET-based peptide substrate for detection of protease activity of coronaviruses

From: 603 USD
Catalog #
100-0505_C
FRET-based peptide substrate for detection of protease activity of coronaviruses

Product Advantages


  • Enables efficient detection of coronavirus protease activity

  • Can be used as a high throughput screening tool for inhibitors of coronavirus proteases

Overview

CoV Protease Substrate-1 TF5 allows for efficient detection of coronavirus (CoV) proteases. This fluorescence resonance energy transfer (FRET)-based peptide substrate is cleaved by 3C-like proteases (3CLᵖʳᵒ), the CoV main protease involved in regulating the coronavirus replication complex. 3CLᵖʳᵒ is also a potential target for therapeutic development.

CoV Protease Substrate-1 TF5 has an excitation wavelength of 649 nm, an emission wavelength of 664 nm, and an extinction coefficient of 250,000 cm⁻¹ M⁻¹. It consists of a 14 amino acid sequence (KTSAVLQSGFRKME) that also contains the Tide Quencher™ 5 (TQ5; quencher) and Tide Fluor™ 5 (TF5; donor) fragments at the N- and C- termini, respectively. When the peptide substrate is intact, favorable energetic overlap of the TF5 excited state and the TQ5 absorption leads to efficient intramolecular fluorescence quenching of TF5 by the TQ5 fragment. Cleavage of the substrate by CoV proteases, however, dissociates the TF5 fragment from TQ5, restoring the fluorescence of the TF5 moiety that is directly correlated to the CoV protease activity.
Application
Enzyme Substrate, FRET
Area of Interest
Drug Discovery and Toxicity Testing, Infectious Diseases

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0505, 100-0506
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0505, 100-0506
Lot #
All
Language
English

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