CoV Protease Substrate-1 EDANS

FRET-based peptide substrate for detection of protease activity of coronaviruses

CoV Protease Substrate-1 EDANS

FRET-based peptide substrate for detection of protease activity of coronaviruses

From: 385 USD
Catalog #
100-0507_C
FRET-based peptide substrate for detection of protease activity of coronaviruses

Product Advantages


  • Enables efficient detection of coronavirus protease activity

  • Can be used as a high throughput screening tool for inhibitors of coronavirus proteases

Overview

CoV Protease Substrate-1 EDANS allows for efficient detection of coronavirus (CoV) proteases. This fluorescence resonance energy transfer (FRET)-based peptide substrate can be cleaved by 3C-like protease (3CLᵖʳᵒ), the CoV main protease involved in regulating the coronavirus replication complex. 3CLᵖʳᵒ is also a potential target for therapeutic applications.

CoV Protease Substrate-1 EDANS has an excitation wavelength of 336 nm, an emission wavelength of 455 nm, and an extinction coefficient of 5900 cm⁻¹ M⁻¹. It consists of a 14 amino acid sequence (KTSAVLQSGFRKME) that also contains the DABCYL (quencher) and EDANS (donor) fragments at the N- and C- termini, respectively. When the peptide substrate is intact, favorable energetic overlap of the EDANS excited state and the DABCYL absorption leads to efficient intramolecular fluorescence quenching of EDANS by the DABCYL fragment. Cleavage of the substrate by CoV proteases, however, dissociates the EDANS fragment from DABCYL, restoring the fluorescence of the EDANS moiety that is directly correlated to the CoV protease activity.
Application
Enzyme Substrate, FRET
Area of Interest
Drug Discovery and Toxicity Testing, Infectious Diseases

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
100-0507, 100-0508
Lot #
All
Language
English
Document Type
Safety Data Sheet
Catalog #
100-0507, 100-0508
Lot #
All
Language
English

Contact STEMCELL Technologies

Our Customer Service, Sales, and Product and Scientific Support departments in North America are available between 6 am and 5 pm Pacific Time (9 am and 8 pm Eastern Time). One of our representatives will be happy to help you by telephone or email. Please complete the form to contact us by email. A representative will get back to you shortly.
  •  

StemCell Technologies Inc. and affiliates ("STEMCELL Technologies") does not share your email address with third parties. StemCell Technologies Inc. will use your email address to confirm your identity and send you newsletters, transaction-related emails, promotional and customer service emails in accordance with our privacy policy. You can change your email preferences at any time.