Organoid Embedding Sheet

Silicone sheet designed to facilitate embedding 3D aggregates in a matrix
Organoid Embedding Sheet (6-Pack)

Silicone sheet designed to facilitate embedding 3D aggregates in a matrix

6 Sheets
Catalog # 08579
35 USD

Overview

The Organoid Embedding Sheet is designed to facilitate embedding 3-dimensional (3D) aggregates (e.g. embryoid bodies, spheroids, or organoids) into a matrix. The wells on the sheet are designed to fit aggregates up to 5 mm in diameter. The sheet may be grasped at the top, along the label, for easy handling. The Organoid Embedding Sheet can also be a substitute for Parafilm® and used to mix small volumes in various protocols. Each sheet contains 18 wells, and the sheet is compatible with STEMCELL media products such as STEMdiff™ Cerebral Organoid Kit (Catalog #08570).
Advantages
• Designed to fit 3D aggregates in a matrix droplet
• Two sheets fit into a 100 mm dish (e.g. Catalog #38045)
• Each sheet contains 18 wells
Contains
Silicone

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
Organoid Embedding Sheet (6-Pack)
Catalog #
08579
Lot #
All
Language
English

Educational Materials (14)

Brochure
2019-2020 Cell Culture Training Catalog
Brochure
hPSC-Derived Neural Cell Research
Brochure
STEMdiff™ Cerebral Organoid Kit
Brochure
Organoids
Technical Bulletin
Cryogenic Tissue Processing and Section Immunofluorescence of Cerebral Organoids
Wallchart
Cell-Reprogramming Technology and Neuroscience
Video
How to Grow Cerebral Organoids from Human Pluripotent Stem Cells
9:18
How to Grow Cerebral Organoids from Human Pluripotent Stem Cells
Webinar
Organoid Expert Panel
52:35
Organoid Expert Panel
Webinar
Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease
25:30
Brains in a Dish: Using Cerebral Organoids to Study Human Brain Development and Disease
Webinar
Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish
47:18
Madeline Lancaster on Brain Organoids: Modeling Human Brain Development in a Dish
Webinar
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
50:49
hPSC Quality: Essential Considerations for Gene Editing, Cloning, Maintenance and Disease Modeling
Scientific Poster
CRISPR-Cas9 Gene Editing Of CDK5RAP2 In Human Pluripotent Stem Cells, Derivation Of Genetically Stable Clonal Lines And Formation Of Cerebral Organoids
Scientific Poster
STEMdiff™ Cerebral Organoid Kit: A New Tool for the Culture of 3D Brain Organoids Derived from hPSCs
Scientific Poster
STEMdiff Cerebral Organoid Kit: A New Tool for the Culture of 3D Brain Organoids Derived from Human Pluripotent Stem Cells
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Data and Publications

Data

(A) A representative phase-contrast image of a whole cerebral organoid at day 40 generated using the STEMdiff™ Cerebral Organoid Kit. Cerebral organoids at this stage are made up of phase-dark structures that may be surrounded by regions of thinner, more translucent structures that display layering (arrowheads). (B) Immunohistological analysis on cryosections of cerebral organoids reveals cortical regions within the organoid labelled by the apical progenitor marker PAX6 (red) and neuronal marker β-tubulin III (green). (C-F) Inset of boxed region from (B). (C) PAX6+ apical progenitors (red, enclosed by dotted line) are localized to a ventricular zone-like region. β-tubulin III+ neurons (green) are adjacent to the ventricular zone. (D) CTIP2, a marker of the developing cortical plate, co-localizes with β-tubulin III+ neurons in a cortical plate-like region. Organization of the layers recapitulates early corticogenesis observed during human brain development. (E) Proliferating progenitor cells labeled by Ki-67 (green) localize along the ventricle, nuclei are counterstained with DAPI (blue). (F) An additional population of Ki-67+ cells is found in an outer subventricular zone-like region (arrowheads). Scale Bar = (A) 1 mm, (B) 500 µm and (C-F) 200 µm.

Principal component analysis of hPSC and cerebral organoid transcriptomes. Cerebral organoids generated using the STEMdiff™ Cerebral Organoid Kit (filled blue circles) cluster together, and cluster with previously published (C Luo et al. Cell Rep, 2016) cerebral organoids (open blue circles). The first principal component accounts for the majority of variance seen (PC1; 80%) and distinguishes the cerebral organoid samples from the hPSCs (green circles). The second principal component accounts for only 9% of the variation, and highlights the modest expression differences between cultured organoids and primary embryonic fetal brain samples (19 post-conceptional weeks, brown circles).

Heatmap of expression levels for genes associated with synaptic transmission function and neurogenesis in Day 40 organoids. These data show that gene expression of cerebral organoids generated from the STEMdiff™ Cerebral Organoid Kit are similar to published results (C Luo et al. Cell Rep, 2016).

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