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Adipocyte-specific adhesion molecule (ASAM) may play a role in cell-cell adhesion as it is expressed in epithelial cells of various tissues and is associated with tight junctions (Raschberger et al., Sze et al.). This type 1 transmembrane protein belongs to the cortical thymocyte marker in the Xenopus (CTX) family within the immunoglobulin superfamily. Sequence alignment analysis has shown that ASAM shares conserved characteristics with other CTX-like proteins, including extracellular V-type and C2-type domains, a transmembrane region, and a cytoplasmic tail (Raschperger et al.). ASAM is also expressed in adipocytes, and was found to be upregulated in white adipose tissue (WAT) in a rodent model of obesity and type II diabetes (Eguchi et al.). This protein contains a His-residue tag at the carboxyl end of the polypeptide chain. For consistency and reproducibility across your applications, adipocyte-specific adhesion molecule from STEMCELL comes lyophilized with ≥ 94% purity, and is verified by LAL analysis to ensure endotoxin levels are ≤ 1.0 EU/μg protein.
Human Recombinant ASAM was resolved with SDS-PAGE under reducing (+) conditions and visualized by Coomassie Blue staining. Human Recombinant ASAM has a predicted molecular mass of 25.8 kDa and an apparent molecular mass of 32 - 35 kDa due to glycosylation.