The Human RANTES (CCL5) ELISA Kit is designed for the quantitative detection and measurement of human RANTES (regulated upon activation, normal T cell expressed and secreted), also known as CCL5, in biological fluids such as serum, plasma, and cell culture supernatants. RANTES is secreted by T cells, fibroblasts, macrophages, epithelial cells, and endothelial cells induced by inflammatory stimuli such as IFN-γ, TNFα, or IL-1. RANTES binds to three different chemokine receptors: CCR1, CCR3 and CCR5. The CCR5 is the main co-receptor for macrophage tropic strains of HIV-1 and HIV-2 and acts as an HIV-suppressor when secreted by CD8+ Tcells. RANTES also acts as an inflammatory mediator inducing lymphocyte and monocyte migration that suppresses pro-malignancy activities in breast cancer.

The assay is based on a sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for human RANTES. The captured RANTES is detected by the addition of a horseradish peroxidase (HRP)-conjugated detection antibody. The addition of the chromogenic enzyme substrate 3,3’5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human RANTES in the sample.