Human IL-6 ELISA Kit
For detection and measurement of human interleukin-6
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Overview
The assay is based on a sandwich ELISA method, in which samples are added to ELISA strip plates pre-coated with capture antibodies specific for human IL-6. The captured IL-6 is detected by the addition of a horseradish peroxidase (HRP)-conjugated detection antibody. The addition of the chromogenic enzyme substrate 3,3’5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the concentration of human IL-6 in the sample.
Data Figures

Figure 1. Representative Standard Curve Of Human IL-6
The reportable range (the concentration range in which measurement of the analyte can be done with the highest precision, accuracy, and linearity) of this assay is 2.81 - 180 pg/mL. The lower limit of detection (LLD) of this assay is 0.58 pg/mL. This is the analyte concentration with absorbance three standard deviations higher than the zero standard. A mid-curve recovery of 97 - 117% or 72 - 78% was determined by spiking defined amounts of analyte standard into cell culture supernatant or serum samples, respectively. The intra-assay precision of this assay is 3.9% (CV). The Inter-assay precision of this assay is 6.4% (CV)
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
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Human IL-6 ELISA Kit
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED.