CoV Protease Substrate-1 TF5

FRET-based peptide substrate for detection of protease activity of coronaviruses
Catalog #
100-0505_C
FRET-based peptide substrate for detection of protease activity of coronaviruses
From: 525 USD

Overview

CoV Protease Substrate-1 TF5 allows for efficient detection of coronavirus (CoV) proteases. This fluorescence resonance energy transfer (FRET)-based peptide substrate is cleaved by 3C-like proteases (3CLᵖʳᵒ), the CoV main protease involved in regulating the coronavirus replication complex. 3CLᵖʳᵒ is also a potential target for therapeutic development.

CoV Protease Substrate-1 TF5 has an excitation wavelength of 649 nm, an emission wavelength of 664 nm, and an extinction coefficient of 250,000 cm⁻¹ M⁻¹. It consists of a 14 amino acid sequence (KTSAVLQSGFRKME) that also contains the Tide Quencher™ 5 (TQ5; quencher) and Tide Fluor™ 5 (TF5; donor) fragments at the N- and C- termini, respectively. When the peptide substrate is intact, favorable energetic overlap of the TF5 excited state and the TQ5 absorption leads to efficient intramolecular fluorescence quenching of TF5 by the TQ5 fragment. Cleavage of the substrate by CoV proteases, however, dissociates the TF5 fragment from TQ5, restoring the fluorescence of the TF5 moiety that is directly correlated to the CoV protease activity.
Advantages
• Enables efficient detection of coronavirus protease activity
• Can be used as a high throughput screening tool for inhibitors of coronavirus proteases

Application
Enzyme Substrate, FRET
Area of Interest
Drug Discovery and Toxicity Testing, Infectious Diseases

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
CoV Protease Substrate-1 TF5
Catalog #
100-0505, 100-0506
Lot #
All
Language
English
Document Type
Safety Data Sheet
Product Name
CoV Protease Substrate-1 TF5
Catalog #
100-0505, 100-0506
Lot #
All
Language
English

Educational Materials(5)

Wallchart
SnapShot: COVID-19
Video
3:36
Collaborating to Accelerate COVID-19 Research
Webinar
1:00:11
Exploring the Impact of SARS-CoV-2 Infection on the Central Nervous System
Webinar
1:10:09
HUB & STEMCELL Organoids as Models of Infectious Disease Mini-Symposium
Webinar
6:56
Studying Respiratory Viruses with ALI Cultures

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