This monoclonal antibody (clone H57-597) reacts with the beta chain of mouse T cell receptor (TCR). TCR beta is a member of the immunoglobulin superfamily and together with the TCR alpha chain forms the alpha-beta TCR. TCR beta is expressed by thymocytes in a developmentally regulated manner and a majority of peripheral T cells. Small number of T cells express an alternative heteromer of gamma and delta protein chains, known as the gamma-delta TCR. TCR-CD3 complex in conjunction with co-receptors CD4 or CD8 enables the recognition and response to antigens attached to major histocompatibility complex (MHC) molecules on antigen-presenting cells. Crosslinking of the TCR complex with H57-597 induces activation and proliferation of T cells or apoptosis based on assay conditions. Use this recombinant monoclonal antibody in applications requiring detection of TCR beta, such as flow cytometry (FC), and the unconjugated antibody in Immunoperoxidase (IP), immunofluorescence (IF), Immunocytochemistry (ICC), Immunohistochemistry (IHC), and FC.
The H57-597 antibody clone has been widely used as a phenotypic indicator for T cells expressing alpha-beta TCR. This antibody does not cross-react with cells expressing the gamma-delta TCR.