Human SARS-CoV-2 Spike Protein Inhibitor Screening Kit

For detection and measurement of compounds that inhibit binding of viral SARS-CoV-2 Spike protein to human ACE2 receptor

Human SARS-CoV-2 Spike Protein Inhibitor Screening Kit

For detection and measurement of compounds that inhibit binding of viral SARS-CoV-2 Spike protein to human ACE2 receptor

From: 1,015 USD
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For detection and measurement of compounds that inhibit binding of viral SARS-CoV-2 Spike protein to human ACE2 receptor
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What's Included

  • Human SARS-CoV-2 Spike Protein Inhibitor Screening Kit(Catalog #100-0700)
    • SARS-CoV-2 Inhibitor Screening Plate, 1x
    • Biotinylated Human ACE2, 10 µg
    • SARS-CoV-2 Spike Protein (RBD), 10 µg
    • SA-HRP, 2 µg
    • Human ACE2 Antibody Positive Control, 20 µL
    • Human IgG HRP-Conjugated Detection Antibody, 120 µL
    • Wash Buffer (10X), 2 x 30 mL
    • ELISA Buffer (10X), 30 mL
    • Blocking Buffer, 24 mL
    • TMB Substrate, 12 mL
    • Stop Solution, 12 mL
    • Adhesive Plate Covers, 2x

Overview

The Human SARS-CoV-2 Spike Protein Inhibitor Screening Kit is designed for the identification and characterization of inhibitor molecules, including antibodies or chemicals, that prevent the binding of SARS-CoV-2 virus to the host angiotensin-converting enzyme 2 (ACE-2)-expressing cells.

The Spike (S) protein is a type I transmembrane glycoprotein present on the surface of coronaviruses (CoV). Entry of CoV, including SARS-CoV-2, into host cells is mediated by the S protein, where it interacts with the cell-surface receptor ACE2. In humans, ACE2 is expressed in several organs and tissues, including the intestinal and respiratory epithelia. The S protein has been shown to play a key role in the induction of neutralizing antibody and T cell responses, which may lead to protective immunity.

This inhibitor screening assay is based on a competitive ELISA method, in which inhibitors to be tested are mixed with biotinylated recombinant human ACE2 and are added to ELISA strip plates coated with recombinant SARS-CoV-2 S protein.. The captured biotinylated ACE2 is detected by streptavidin-horseradish peroxidase (HRP), which binds the biotinylated ACE2. Addition of the chromogenic enzyme substrate 3,3’,5,5’ tetramethylbenzidine (TMB) results in a colored product with an intensity directly proportional to the binding activity of ACE2 to the S protein. Inhibitors will compete with the biotinylated ACE2 for binding to the SARS-CoV-2 S protein, resulting in reduced signal for more effective inhibitors.

This inhibitor screening assay can be performed in the reverse configuration, in which the plate is coated with biotinylated recombinant ACE2, recombinant S Protein is mixed with the inhibitor to be tested, and S protein binding is detected using anti-human IgG-HRP.
Subtype
Complete Kits
Application
ELISA
Area of Interest
Drug Discovery and Toxicity Testing, Infectious Diseases

Data Figures

Graph showing absorbance (450 nm) values at different inhibitor concenration (ng/mL)

Figure 1. Performance of the Human SARS-CoV-2 Spike Protein Inhibitor Screening Kit

Binding of biotinylated human ACE2 to the Spike protein of SARS-CoV-2 coated on the ELISA plate is inhibited by the anti-human ACE2 antibody (clone AC384) positive control (orange line). The gray line represents a non-inhibitory negative control.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

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Safety Data Sheet 1
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Safety Data Sheet 9
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