EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit

Immunomagnetic negative selection cell isolation kit

New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.

EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit

Immunomagnetic negative selection cell isolation kit

From: 459 USD
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Immunomagnetic negative selection cell isolation kit
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Product Advantages


  • Fast and easy-to-use

  • No columns required

  • Untouched, viable cells

What's Included

  • EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Catalog #19860)
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL
  • RoboSep™ Mouse Streptavidin RapidSpheres™ Isolation Kit (Catalog #19860RF)
    • EasySep™ Streptavidin RapidSpheres™ 50001, 1 mL
    • EasySep™ Mouse FcR Blocker (Catalog #18731), 0.5 mL
    • RoboSep™ Empty Vial
    • RoboSep™ Buffer (Catalog #20104)
    • RoboSep™ Filter Tips (Catalog #20125)

Overview

The EasySep™ Mouse Streptavidin RapidSpheres™ Isolation Kit is designed for the depletion of single or multiple unwanted cell types labeled with biotinylated antibodies. Labeled cells from mouse splenocytes or other tissues are targeted for removal by Streptavidin RapidSpheres™ and separated without the use of columns using an EasySep™ magnet. Desired cells are untouched and poured off into a new tube.
Magnet Compatibility
• EasySep™ Magnet (Catalog #18000)
• “The Big Easy” EasySep™ Magnet (Catalog #18001)
• RoboSep™-S (Catalog #21000)
Subtype
Cell Isolation Kits
Cell Type
B Cells, Dendritic Cells, Granulocytes and Subsets, Hematopoietic Stem and Progenitor Cells, Macrophages, Marrow Stromal Cells, Mesenchymal Stem and Progenitor Cells, Monocytes, Mononuclear Cells, Myeloid Cells, NK Cells, Other, Plasma, T Cells
Species
Mouse
Sample Source
Other, Spleen
Selection Method
Depletion, Negative
Application
Cell Isolation
Brand
EasySep, RoboSep
Area of Interest
Immunology

Data Figures

Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Figure 1. Typical Mouse Streptavidin Rapidspheres™ CD4 (CD3+CD8-) Depletion Profile

Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Figure 2. Typical Mouse Streptavidin Rapidspheres™ CD8 (CD3+CD4-) Depletion Profile

Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

Figure 3. Typical Mouse Streptavidin Rapidspheres™ CD19 (CD19+CD45+) Depletion Profile

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Catalog #
19860RF
Lot #
1000147072 or higher
Language
English
Catalog #
19860RF
Lot #
1000147071 or lower
Language
English
Catalog #
19860
Lot #
1000147072 or higher
Language
English
Catalog #
19860
Lot #
1000147071 or lower
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 4
Catalog #
19860RF
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Catalog #
19860
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Catalog #
19860
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Catalog #
19860
Lot #
All
Language
English

Resources and Publications

Publications (7)

Repurposing a novel anti-cancer RXR agonist to attenuate acute GVHD and maintain graft-versus-leukemia responses. G. Thangavelu et al. Blood 2020 sep

Abstract

The nuclear receptors (NR) retinoid X receptors (RXRs) exert immunomodulatory functions to control inflammation and metabolism via homodimers and heterodimers with several other NRs including retinoic acid receptors. IRX4204 is a novel, highly specific RXR agonist in clinical trials that potently and selectively activates RXR homodimers but not heterodimers. Here, we show that in vivo IRX4204 was compared favorably with FK506 in abrogating acute graft-versus-host disease (GVHD), which was associated with inhibiting allogeneic donor T cell proliferation, reducing T helper 1 differentiation and promoting regulatory T cell (Treg) generation. Recipient IRX4204 treatment reduced intestinal injury and decreased IFN-$\gamma$ and TNF-$\alpha$ serum levels. Transcriptional analysis of donor T cells isolated from intestines of GVHD mice treated with IRX4204 revealed significant decreases in transcripts regulating pro-inflammatory pathways. In vitro, inducible Treg differentiation from na{\{i}}ve CD4+ T cells was enhanced by IRX4204; in vivo IRX4204 increased the conversion of donor Foxp3- T cells into peripheral Foxp3+ Tregs in GVHD mice. Using Foxp3 lineage tracer mice in which both the origin and current FoxP3 expression of Tregs can be tracked we demonstrate that IRX4204 supported Treg stability. Despite favoring Tregs and reducing Th1 differentiation IRX4204-treated recipients maintained graft-versus-leukemia responses against both leukemia and lymphoma cells. Notably IRX4204 reduced in vitro human T cell proliferation and enhanced Treg generation in mixed lymphocyte reaction cultures. Collectively these beneficial effects indicate that targeting RXRs with IRX4204 could be used as a novel approach to prevent acute GVHD in the clinic."
Tumor-Resident Stromal Cells Promote Breast Cancer Invasion through Regulation of the Basal Phenotype. C. J. Hanley et al. Molecular cancer research : MCR 2020 nov

Abstract

Collective invasion can be led by breast cancer cells expressing basal epithelial markers, typified by keratin-14 (KRT14). We analyzed gene expression data from The Cancer Genome Atlas and demonstrated a significant correlation between a KRT14+ invasion signature and a stromal-mediated extracellular matrix (ECM) organization module. We then developed a novel coculture model of tumor organoids with autologous stromal cells. Coculture significantly increased KRT14 expression and invasion of organoids from both luminal and basal murine breast cancer models. However, stromal cell conditioned medium induced invasion but not KRT14 expression. Cancer cells released TGF$\beta$ and that signaling pathway was required for stromal cell-induced invasion and KRT14 expression. Mechanistically, TGF$\beta$ induced NOX4 expression in stromal cells and NOX4 inhibition reduced invasion and KRT14 expression. In summary, we developed a novel coculture model and revealed dynamic molecular interactions between stromal cells and cancer cells that regulate both basal gene expression and invasive behavior. IMPLICATIONS: Fibroblasts within mammary tumors can regulate the molecular phenotype and invasive behavior of breast cancer cells. VISUAL OVERVIEW: http://mcr.aacrjournals.org/content/molcanres/18/11/1615/F1.large.jpg.
Complete Topological Mapping of a Cellular Protein Interactome Reveals Bow-Tie Motifs as Ubiquitous Connectors of Protein Complexes. K. Niss et al. Cell reports 2020 jun

Abstract

The network topology of a protein interactome is shaped by the function of each protein, making it a resource of functional knowledge in tissues and in single cells. Today, this resource is underused, as complete network topology characterization has proved difficult for large protein interactomes. We apply a matrix visualization and decoding approach to a physical protein interactome of a dendritic cell, thereby characterizing its topology with no prior assumptions of structure. We discover 294 proteins, each forming topological motifs called bow-ties" that tie together the majority of observed protein complexes. The central proteins of these bow-ties have unique network properties display multifunctional capabilities are enriched for essential proteins and are widely expressed in other cells and tissues. Collectively the bow-tie motifs are a pervasive and previously unnoted topological trend in cellular interactomes. As such these results provide fundamental knowledge on how intracellular protein connectivity is organized and operates."
New format, same high quality! You may notice that your kit contents and packaging look slightly different from previous orders. We are currently updating the format of select EasySep™ Mouse kits to include a Mouse FcR blocker instead of Normal Rat Serum. With this change, all components will now be shipped in a single package, while providing the same cell isolation performance as before.