The Big Easy EasySep® Magnet

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Immunomagnetic Column-Free Magnet

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  • "The Big Easy" EasySep® Magnet
"The Big Easy" EasySep® Magnet
"The Big Easy" EasySep® Magnet is designed for cell separation procedures using EasySep® reagents when processing a large number of cells, or when using RoboSep®. "The Big Easy" EasySep® Magnet generates a high-gradient magnetic field in the interior cavity that is strong enough to separate cells labeled with EasySep® Magnetic Particles without the use of columns. "The Big Easy" EasySep® Magnet is designed to hold a standard 17 x 100 mm (14 mL) polystyrene tube.
Product Name Description Catalog # Size Price Quantity
The Big Easy EasySep® Magnet Immunomagnetic column-free magnet 18001 For isolating up to 1 x 10e9 cells 1142.00 USD      
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Order Offline:

Call us: +1.604.877.0713  
Toll Free: 1.800.667.0322  
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Fax us: +1.604.877.0704
Toll Free Fax: 1.800.567.2899

Recommended for:
Processing up to 1 x 10e9 cells per separation (if selecting rare cells such as CD34+ cells, up to 2 x 10e9 cells may be processed per separation), or for processing up to 4.5 mL whole blood
Intended Use Statement: For Research Use Only. Not for Therapeutic or Diagnostic Use.
Product Type: Cell isolation products
Popular Product Line: EasySep
Cell Isolation Product Type: Equipment

Procedures and instruction manuals:

Educational resources:

FAQS:


  • Q. HOW DOES THE SEPARATION WORK?

    A. Tiny magnetic particles are attached to cells. When placed in the EasySep® Magnet, the cells migrate to the wall of the tube.

  • Q. WHICH COLUMNS DO I USE?

    A. None, columns are not used in the EasySep® procedure.

  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?

    A. Yes, but this may affect separation performance.

  • Q. IN POSITIVE SELECTION CAN I DO MORE THAN 3 SEPARATIONS TO INCREASE PURITY?

    A. Yes, purity of targeted cells will increase with additional rounds of separations, however the recovery of targeted cells may decrease.

  • Q. ARE EASYSEP® MAGNETIC NANOPARTICLES FACS-COMPATIBLE?

    A. Yes, the EasySep® particles are flow cytometry-compatible, very uniform in size and ~5000X smaller than other commercially available magnetic beads used with column-free systems.

  • Q. CAN THE PARTICLE BE REMOVED AFTER ENRICHMENT?

    A. No, but they are so tiny, they do not interfere with downstream applications.

  • Q. CAN EASYSEP® BE USED TO ISOLATE RARE CELLS?

    A. Yes, however we recommend a cell concentration of 2X108 cells/ml and a minimum working volume of 100 µL. 2x107 cells or fewer should be suspended in 100 µL of buffer.

  • Q. CAN EASYSEP® BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?

    A. Yes, we have products for both.

  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?

    A. Each Product Information Sheet has detailed staining information.

  • Q. HOW DOES THE BINDING OF THE NANOPARTICLE AFFECT THE FUNCTION OF CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?

    A. No, we have found that cells are not functionally altered.

  • Q. CAN EASYSEP® SEPARATIONS BE AUTOMATED?

    A. Yes, click here for a link to RoboSep®.

Product Name

Description

Catalog #
EasySep® Magnet Immunomagnetic Column-Free Magnet 18000
Easy 50 EasySep® Magnet Immunomagnetic Column-Free Magnet 18002
RoboSep® RoboSep® - The Fully Automated Cell Separator 20000
EasySep® Multistand Immunomagnetic Column-Free Multistand 18010

Typical EasySep® Procedure for Human Cells


Typical EasySep® Procedure for Human Cells



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