Regulatory T cell isolation

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Treg Wallchart


EasySep Sample

Regulatory T Cell Separation The first step towards quality Treg research is the isolation of highly purified, functional Tregs. STEMCELL has now developed a new range of Treg isolation kits for the rapid and efficient isolation of human Tregs. Choose the best Treg kit based on your desired phenotype or Treg subtype, your downstream application and your starting sample, or let STEMCELL design a kit that is tailor-made for your research.
 
Classic Tregs

Get the fastest isolation of highly purified CD4+CD25high T cells ideal for use either directly in downstream assays, or for reducing flow sorting time.
Recommended Products:
Complete Kit for CD4+CD25+ T Cells
EasySep® Human CD4+CD25+ T Cell Isolation Kit

 
Highest Purity Tregs

CD127 and CD49d are useful cell surface markers for isolating Foxp3 Tregs by negative selection. STEMCELL has developed a variety of kits using these markers to give you the highest purity of Foxp3 cells for downstream analysis without the need for flow sorting.
Recommended Products:
Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells
Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells
 
Untouched Tregs

For researchers who need a gentle method of isolating untouched Tregs, STEMCELL has developed negative selection kits that enrich for CD4 cells which do not express CD127 (or CD49d). Our fastest isolation kits give highly functional, untouched Tregs in a single step from PBMCs in one hour.
Recommended Products:
EasySep® Human CD4+CD127low T Cell Enrichment Kit
EasySep® Human CD4+CD127lowCD49d- T Cell Enrichment Kit

 
Pre-enrichment of Tregs

When purities of over 90% are required, STEMCELL’s pre-enriched kits are an ideal way to save hours on flow sorting while getting purities you need. RosetteSep® or EasySep® Pre-Enrichment Kits provide excellent yields of pre-enriched Tregs from whole blood, buffy coat or PBMC and allow you to go from sample to sorting quickly.
Recommended Products:
RosetteSep® Human CD4+ T Cell Enrichment Cocktail
RosetteSep® Human CD4+CD127low T Cell Enrichment Cocktail
EasySep® Human CD4+CD127low T Cell Enrichment Kit
EasySep® Human CD4+ T Cell Enrichment Kit
EasySep® Mouse CD4+ T Cell Pre-Enrichment Kit

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Product Name Description Catalog # Size Price Quantity
Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells Complete kit for human CD4+CD127lowCD25+ regulatory T cells 15861 For labeling 200 mL of whole blood 741.00 USD
Complete Kit for CD4+CD25+ T Cells Complete kit for human CD4+CD25+ regulatory T cells 15862 For labeling 200 mL of whole blood 686.00 USD
Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells Complete kit for human CD4+CD127lowCD49d-CD25+ regulatory T cells 15864 For labeling 200 mL of whole blood 741.00 USD
EasySep® Human CD25 Positive Selection Kit Immunomagnetic positive selection of human CD25high cells 18231 For labeling up to 8 x 10e8 total cells 209.00 USD
EasySep® Human CD4+CD127low T Cell Enrichment Kit Immunomagnetic isolation of untouched human CD4+CD127low T cells 19231 For labeling up to 1 x 10e9 cells 668.00 USD
EasySep® Human CD4+CD25 T Cell Isolation Kit Immunomagnetic positive selection of human CD4+CD25+ T cells 18062 For labeling up to 1 x 10e9 cells 824.00 USD
EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Immunomagnetic selection of mouse CD4+CD25+ regulatory T cells 19782 For labeling up to 1 x 10e9 cells 628.00 USD
EasySep® Mouse CD4+CD25+ Regulatory T Cell Isolation Kit Immunomagnetic selection of mouse CD4+CD25+ regulatory T cells 19792 For labeling up to 4 x 10e9 cells 2453.00 USD
EasySep™ Human CD4+CD127lowCD49d- Regulatory T Cell Enrichment Kit Immunomagnetic isolation of untouched human CD4+CD127lowCD49d- T cells 19232 For labeling up to 2 x 10e9 cells 792.00 USD
EasySep™ Human Pan-CD25 Positive Selection and Depletion Kit Immunomagnetic positive selection or depletion of human CD25+ cells 18251 For labeling up to 1 x 10e9 cells 592.00 USD
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