NeuroCult™ SM1 Neuronal Supplement

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Supplement for the Culture of Primary Neurons

  • NeuroCult™ SM1 Neuronal Supplement
  • Vial label for NeuroCult® SM1 Neuronal Supplement 10 mL
  • NeuroCult SM1 Neurons
NeuroCult™ SM1 Neuronal Supplement
NeuroCult™ SM1 Neuronal Supplement is a standardized serum-free supplement for the culture of mouse and rat primary neurons. The formulation of NeuroCult™ SM1 Neuronal Supplement is based on the published B27 formulation (Brewer et al.), but optimized to more reproducibly support the survival of mature neurons in long-term culture. Neurons cultured for 21 days using NeuroCult™ SM1 Neuronal Supplement show increased cell survival, neurite outgrowth and neurite branching, compared to a traditional serum-free medium.
 
NOTE: Requires the addition of L-glutamine (Catalog #07100) and L-glutamic acid.
Product Name Description Catalog # Size Price Quantity
NeuroCult™ SM1 Neuronal Supplement Supplement for the culture of primary neurons 05711 10 mL 82.00 USD      
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Product Type: Specialized cell culture media
Recommended For:
Culture of primary neurons at low or high cell densities in both short- and long-term cultures
Components:
N/A
Accessory Products: L-glutamine (Catalog #07100)
Contains:
Antioxidants and retinoic acid
Equipment Required:
N/A
Advantages:
• NeuroCult™ SM1 is formulated to support improved cell survival in long-term primary neuronal culture.
• Cultures feature increased neurite outgrowth and branching in short- and long-term cultures.
• Product undergoes rigorous performance testing with primary neuronal cultures.
Area of Interest: Neuroscience
Cell Type: Neurons
Popular Product Line: NeuroCult™
Species: Mouse, Rat
Legal Statement:
N/A
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

This product has been used in:

  1. Barbara Calabrese et al. Activity-Dependent Dendritic Spine Shrinkage and Growth Involve Downregulation of Cofilin via Distinct Mechanisms.PloS one 9 (4) e94787 (2014)
  2. Graciano Leal et al. Neuronal Activity Induces Synaptic Delivery of hnRNP A2/B1 by a BDNF-Dependent Mechanism in Cultured Hippocampal Neurons.PloS one 9 (10) e108175 (2014)
  3. Jimcy Platholi et al. Isoflurane reversibly destabilizes hippocampal dendritic spines by an actin-dependent mechanism.PloS One 9 (7) e102978 (2014)
  4. Joana Fernandes et al. In vitro ischemia triggers a transcriptional response to down-regulate synaptic proteins in hippocampal neurons.PloS One 9 (6) e99958 (2014)
  5. G Stefano Brigidi et al. Palmitoylation of δ-catenin by DHHC5 mediates activity-induced synapse plasticity.Nat Neurosci 17 (4) 522-32 (April 2014)
  6. M Vieira et al. Ischemic insults induce necroptotic cell death in hippocampal neurons through the up-regulation of endogenous RIP3.Neurobiology of disease (April 16, 2014)
  7. Amanda N Sacino et al. Amyloidogenic α-synuclein seeds do not invariably induce rapid, widespread pathology in mice.Acta neuropathologica (March 23, 2014)
  8. Jessica K Lerch et al. cJun promotes CNS axon growth.Mol Cell Neurosci (February 9, 2014)
  9. Veronica J Jessick et al. Investigating the role of the actin regulating complex ARP2/3 in rapid ischemic tolerance induced neuro-protection.International journal of physiology, pathophysiology and pharmacology 5 (4) 216-27 (2013)
  10. Ida Annunziata et al. Lysosomal NEU1 deficiency affects amyloid precursor protein levels and amyloid-β secretion via deregulated lysosomal exocytosis.Nat Commun 4 2734 (2013)
  11. X Qu et al. Differentiation of reprogrammed human adipose mesenchymal stem cells toward neural cells with defined transcription factors.Biochem Biophys Res Commun (September 11, 2013)
  12. Helena Bujalka et al. MYRF Is a Membrane-Associated Transcription Factor That Autoproteolytically Cleaves to Directly Activate Myelin Genes.PLoS Biol 11 (8) e1001625 (August 13, 2013)
  13. H Al-Ali et al. Chemical Interrogation of the Neuronal Kinome Using a Primary Cell-Based Screening Assay.ACS Chem Biol () (March 19, 2013)
  14. K J Lee et al. Mossy fiber-CA3 synapses mediate homeostatic plasticity in mature hippocampal neurons.Neuron 77 (1) 99-114 (January 9,2013)
  15. Saar Oz et al. The ADNP Derived Peptide, NAP Modulates the Tubulin Pool: Implication for Neurotrophic and Neuroprotective Activities.PLoS One 7 (12) e51458 (2012)
  16. Kevin She et al. NMDA receptors mediate synaptic competition in culture.PLoS One 6 (9) e24423 (2011)
  17. Frederick Dobie et al. Inhibitory synapse dynamics: coordinated presynaptic and postsynaptic mobility and the major contribution of recycled vesicles to new synapse formation.The Journal of Neuroscience 31 (29) 10481-10493 (July 20, 2011)
  18. PC Rosato et al. Intrinsic innate immunity fails to control herpes simplex and vesicular stomatitis virus replication in sensory neurons and fibroblasts.J Virol () (June 18, 2014)

Background References:

  1. G J Brewer et al. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination.J Neurosci Res 35 (5) 567-576 (August 1, 1993)

Product Name

Description

Catalog #

NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue 05715
MAP2 Antibody, Clone AP20 Mouse Monoclonal Antibody to Microtubule-Associated Protein 2 (MAP2) 01410
Neuronal Class III beta-Tubulin Antibody, Clone TUJ1 Mouse Monoconal Antibody to Neuronal Class III β-Tubulin 01409
Tyrosine Hydroxylase Antibody, Clone TH-2 Mouse Monoclonal Antibody to Tyrosine Hydroxylase 01412
NGF Receptor/p75 (CD271) Antibody, Clone MC192 Mouse Monoclonal Antibody to NGF Receptor, p75 (CD271) 01464
L-Glutamine L-Glutamine (200 mM) 07100
NeuroCult™ SM2 Neuronal Supplement (Substrate-Independent) Substrate-Independent Supplement for Culture of Primary Neurons 05721
NeuroCult™ SM1 Neuronal Culture Kit Serum-Free Medium for Culture of Primary Neurons 05712

Morphology of Neurons in Representative NeuroCult™ SM1 Cultures at 7 and 21 Days in Vitro.


Morphology of Neurons in Representative NeuroCult™  SM1 Cultures at 7 and 21 Days in Vitro.
Primary rat E18 cortical neurons were cultured for 7 (A) and 21 (B) DIV in NeuroCult™ SM1 medium. (A) Phase contrast imaging at 7 DIV shows large numbers of viable neurons, with minimal cell clumping and extensive neurite outgrowth and branching. (B) After 21 DIV, large numbers of viable neurons with developed dendritic arbors remain in culture. Magnification 20x.


Number of Neurons in NeuroCult™ SM1 and TSFM Cultures After 7 and 21 Days in Vitro.


Number of Neurons in NeuroCult™ SM1 and TSFM  Cultures After 7 and 21 Days in Vitro.
(A) Comparable numbers of neurons are obtained when cells are cultured for 7 days in NeuroCult™ SM1 compared to TSFM (n = 25; mean ± 95% CI; p > 0.05). (B) Significantly higher numbers of neurons are obtained when cells are cultured for 21 days in NeuroCult™ SM1 compared to TSFM (n = 25; mean ± 95% CI; ***p < 0.001).


Neurite Outgrowth of Primary Neurons Cultured in NeuroCult™ SM1 and TSFM for 7 and 21 Days.


Neurite Outgrowth of Primary Neurons Cultured in  NeuroCult™ SM1 and TSFM for 7 and 21 Days.
Significantly longer neurite outgrowth was observed for cells cultured for 7 (A) and 21 (B) days in NeuroCult™ SM1 compared to TSFM (n = 240 independent measures; mean ± 95% CI; ***p < 0.001).


Neurite Branching of Primary Neurons Cultured in NeuroCult™ SM1 and TSFM for 7 and 21 Days.


Neurite Branching of Primary Neurons Cultured in  NeuroCult™ SM1 and TSFM for 7 and 21 Days.
Significantly more neurite branch points were observed for cells cultured for 7 (A) and 21 (B) days in NeuroCult™ SM1 compared to TSFM (n = 240 independent measures; mean ± 95% CI; ***p < 0.001).


Expression of Pre- and Post-Synaptic Markers in Neurons Cultured for 21 Days in NeuroCult™ SM1.


Expression of Pre- and Post-Synaptic Markers in Neurons  Cultured for 21 Days in NeuroCult™ SM1.
Neurons cultured in NeuroCult™ SM1 for 21 days are phenotypically mature as indicated by the presence of an extensive dendritic arbor and appropriate expression and localization of pre- (Synapsin) and post-synaptic (PSD-95) markers. (A-C) Synapsin (green) staining is concentrated in discrete puncta distributed along the somata and dendritic processes, as defined by MAP2 (red) staining. (D-F) Dendritic staining observed for MAP2 and punctate staining for the postsynaptic marker PSD-95. Nuclei were counter-stained with DAPI. Scale bar 10 μm.


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