NeuroCult™ SM1 Neuronal Supplement

Product Type:	Specialized cell culture media
Recommended for:	Culture of primary neurons at low or high cell densities in both short- and long-term cultures
Components:	N/A
Accessory Products:	L-glutamine (Catalog #07100)
Contains:
Equipment Required:	N/A
Area of Interest:	Neuroscience
Cell Type:	Neurons
Popular Product Line:	NeuroCult™
Species:	Mouse, Rat
Legal Statement:	N/A
Intended Use Statement:	FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 05711, Lot# All:29918-PIS_2_2_0.pdf

Educational resources:

•  SCIENTIFIC POSTER: A new defined serum-free medium formulation to culture mature neurons at higher yields from primary embryonic mouse CNS
•  BROCHURE: Primary Neuronal Culture: Standardized Media and Reagents
•  TECH BULLETIN: Semi-Automated Neurite Analysis Of Neurons Cultured With NeuroCult™ SM
•  SCIENTIFIC POSTER: Efficient new serum-free culture media for culturing high yields of functional mature neurons from primary embryonic mouse central nervous system tissues
•  BROCHURE: Training Courses 2013
•  SCIENTIFIC POSTER: Complete serum-free culture kit and protocols for culturing high yields of functional mature neurons from primary embryonic mouse CNS tissues (Neuroscience 2012)

MSDS:

•  05711-MSDS.pdf

This product has been used in:

1. H Al-Ali et al. Chemical Interrogation of the Neuronal Kinome Using a Primary Cell-Based Screening Assay.ACS Chem Biol () (March 19, 2013)
2. K J Lee et al. Mossy fiber-CA3 synapses mediate homeostatic plasticity in mature hippocampal neurons.Neuron 77 (1) 99-114 (January 9,2013)
3. Saar Oz et al. The ADNP Derived Peptide, NAP Modulates the Tubulin Pool: Implication for Neurotrophic and Neuroprotective Activities.PLoS One 7 (12) e51458 (2012)
4. Kevin She et al. NMDA receptors mediate synaptic competition in culture.PLoS One 6 (9) e24423 (2011)
5. Frederick Dobie et al. Inhibitory synapse dynamics: coordinated presynaptic and postsynaptic mobility and the major contribution of recycled vesicles to new synapse formation.The Journal of Neuroscience 31 (29) 10481-10493 (July 20, 2011)

Background References:

1. G J Brewer et al. Optimized survival of hippocampal neurons in B27-supplemented Neurobasal, a new serum-free medium combination.J Neurosci Res 35 (5) 567-576 (August 1, 1993)

Product Name	Description	Catalog #
NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat)	Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue	05715
MAP2 Antibody, Clone AP20	Mouse Monoclonal Antibody to Microtubule-Associated Protein 2 (MAP2)	01410
Neuronal Class III beta-Tubulin Antibody, Clone TUJ1	Mouse Monoconal Antibody to Neuronal Class III β-Tubulin	01409
NGF Receptor/p75 (CD271) Antibody, Clone MLR-2	Mouse Monoclonal Antibody to NGF Receptor, p75NTR (CD271)	01463
NGF Receptor/p75 (CD271) Antibody, Clone MLR-2, FITC-Conjugated	Mouse Monoclonal Antibody to NGF Receptor, p75NTR (CD271) - FITC Conjugated	10428
Tyrosine Hydroxylase Antibody, Clone TH-2	Mouse Monoclonal Antibody to Tyrosine Hydroxylase	01412
NGF Receptor/p75 (CD271) Antibody, Clone MC192	Mouse Monoclonal Antibody to NGF Receptor, p75 (CD271)	01464
L-Glutamine	L-Glutamine (200 mM)	07100
NeuroCult™ SM2 Neuronal Supplement (Substrate-Independent)	Substrate-Independent Supplement for Culture of Primary Neurons	05721
NeuroCult™ SM1 Neuronal Culture Kit	Serum-Free Medium for Culture of Primary Neurons	05712

NeuroCult™ SM1-supplemented medium efficiently supports substrate-independent culture of neurons (mean ± SEM; n = 11).

E14 mouse cortical neurons were cultured for 6 days in NeuroCult™ Neuronal Basal Medium supplemented with NeuroCult™ SM1, or a traditional serum-free medium (TSFM) on poly-D-lysine/laminin-coated plates. Neurons were detected with a mouse monoclonal β-tubulin III antibody and their cell bodies detected with DAPI. A neuronal profiling software analysis program was used to detect and quantify β-tubulin III+ DAPI+ cells.

Significantly longer neurite outgrowth is observed when neurons are cultured in NeuroCult™ SM1-suplemented medium, compared to a traditional serum-free medium (mean ± SEM; n = 11; p = 0.03).

E14 mouse cortical neurons were cultured for 6 days in NeuroCult™ Neuronal Basal Medium supplemented with NeuroCult™ SM1, or a traditional serum-free medium (TSFM) on poly-D-lysine/laminin-coated plates. Neurons were detected with a mouse monoclonal β-tubulin III antibody and their cell bodies detected with DAPI. A neuronal profiling software analysis program was used to detect and quantify β-tubulin III+ DAPI+ cells.

Significantly more neurite branch points are observed when neurons are cultured in NeuroCult™ SM1-suplemented medium, compared to a traditional serum-free medium (mean ± SEM; n = 11; p = 0.01).

E14 mouse cortical neurons were cultured for 6 days in NeuroCult™ Neuronal Basal Medium supplemented with NeuroCult™ SM1, or a traditional serum-free medium (TSFM) on poly-D-lysine/laminin-coated plates. Neurons were detected with a mouse monoclonal β-tubulin III antibody and their cell bodies detected with DAPI. A neuronal profiling software analysis program was used to detect and quantify β-tubulin III+ DAPI+ cells.