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NeuroCult™ SM1 Neuronal Supplement

Supplement for the Culture of Primary Neurons

  • NeuroCult™ SM1 Neuronal Supplement
  • Vial label for NeuroCult® SM1 Neuronal Supplement 10 mL
  • NeuroCult SM1 Neurons
NeuroCult™ SM1 Neuronal Supplement
Product Catalog # Size Price
NeuroCult™ SM1 Neuronal Supplement
Supplement for the culture of primary neurons
05711 10 mL 82.00 USD
 

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  • NeuroCult™ SM1 Neuronal Supplement (05711)
    10 mL
    82.00 USD

Description


NeuroCult™ SM1 Neuronal Supplement is a standardized serum-free supplement for the culture of mouse and rat primary neurons. The formulation of NeuroCult™ SM1 Neuronal Supplement is based on the published B27 formulation (Brewer et al.), but optimized to more reproducibly support the survival of mature neurons in long-term culture. Neurons cultured for 21 days using NeuroCult™ SM1 Neuronal Supplement show increased cell survival, neurite outgrowth and neurite branching, compared to a traditional serum-free medium.
 
 
NOTE: Requires the addition of L-glutamine (Catalog #07100) and L-glutamic acid.
Advantages:
• NeuroCult™ SM1 is formulated to support improved cell survival in long-term primary neuronal culture.
• Cultures feature increased neurite outgrowth and branching in short- and long-term cultures.
• Product undergoes rigorous performance testing with primary neuronal cultures.
Product Type: Specialized cell culture media
Recommended For:
Culture of primary neurons at low or high cell densities in both short- and long-term cultures
Components:
N/A
Accessory Products: L-glutamine (Catalog #07100)
Contains:
Antioxidants and retinoic acid
Equipment Required:
N/A
Area of Interest: Neuroscience
Cell Type: Neurons
Popular Product Line: NeuroCult™
Species: Mouse, Rat
Legal Statement:
N/A
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Data


Morphology of Neurons in Representative NeuroCult™ SM1 Cultures at 7 and 21 Days in Vitro.


Morphology of Neurons in Representative NeuroCult™  SM1 Cultures at 7 and 21 Days in Vitro.
Primary rat E18 cortical neurons were cultured for 7 (A) and 21 (B) DIV in NeuroCult™ SM1 medium. (A) Phase contrast imaging at 7 DIV shows large numbers of viable neurons, with minimal cell clumping and extensive neurite outgrowth and branching. (B) After 21 DIV, large numbers of viable neurons with developed dendritic arbors remain in culture. Magnification 20x.


Number of Neurons in NeuroCult™ SM1 and TSFM Cultures After 7 and 21 Days in Vitro.


Number of Neurons in NeuroCult™ SM1 and TSFM  Cultures After 7 and 21 Days in Vitro.
(A) Comparable numbers of neurons are obtained when cells are cultured for 7 days in NeuroCult™ SM1 compared to TSFM (n = 25; mean ± 95% CI; p > 0.05). (B) Significantly higher numbers of neurons are obtained when cells are cultured for 21 days in NeuroCult™ SM1 compared to TSFM (n = 25; mean ± 95% CI; ***p < 0.001).


Neurite Outgrowth of Primary Neurons Cultured in NeuroCult™ SM1 and TSFM for 7 and 21 Days.


Neurite Outgrowth of Primary Neurons Cultured in  NeuroCult™ SM1 and TSFM for 7 and 21 Days.
Significantly longer neurite outgrowth was observed for cells cultured for 7 (A) and 21 (B) days in NeuroCult™ SM1 compared to TSFM (n = 240 independent measures; mean ± 95% CI; ***p < 0.001).


Neurite Branching of Primary Neurons Cultured in NeuroCult™ SM1 and TSFM for 7 and 21 Days.


Neurite Branching of Primary Neurons Cultured in  NeuroCult™ SM1 and TSFM for 7 and 21 Days.
Significantly more neurite branch points were observed for cells cultured for 7 (A) and 21 (B) days in NeuroCult™ SM1 compared to TSFM (n = 240 independent measures; mean ± 95% CI; ***p < 0.001).


Expression of Pre- and Post-Synaptic Markers in Neurons Cultured for 21 Days in NeuroCult™ SM1.


Expression of Pre- and Post-Synaptic Markers in Neurons  Cultured for 21 Days in NeuroCult™ SM1.
Neurons cultured in NeuroCult™ SM1 for 21 days are phenotypically mature as indicated by the presence of an extensive dendritic arbor and appropriate expression and localization of pre- (Synapsin) and post-synaptic (PSD-95) markers. (A-C) Synapsin (green) staining is concentrated in discrete puncta distributed along the somata and dendritic processes, as defined by MAP2 (red) staining. (D-F) Dendritic staining observed for MAP2 and punctate staining for the postsynaptic marker PSD-95. Nuclei were counter-stained with DAPI. Scale bar 10 μm.


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Other Reagents and Materials


Product Name

Description

Catalog #

NeuroCult™ SM1 Without Vitamin A Versatile serum-free neural supplement without vitamin A 05731
NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat) Kit for Enzymatic Dissociation of Adult Mouse and Rat CNS Tissue 05715
L-Glutamine L-Glutamine (200 mM) 07100
NeuroCult™ SM2 Neuronal Supplement (Substrate-Independent) Substrate-Independent Supplement for Culture of Primary Neurons 05721
NeuroCult™ SM1 Neuronal Culture Kit Serum-Free Medium for Culture of Primary Neurons 05712

Antibodies


Product Name

Description

Catalog #

MAP2 Antibody, Clone AP20 Mouse Monoclonal Antibody to Microtubule-Associated Protein 2 (MAP2) 01410
Neuronal Class III beta-Tubulin Antibody, Clone TUJ1 Mouse Monoconal Antibody to Neuronal Class III β-Tubulin 01409
Tyrosine Hydroxylase Antibody, Clone TH-2 Mouse Monoclonal Antibody to Tyrosine Hydroxylase 01412
NGF Receptor/p75 (CD271) Antibody, Clone MC192 Mouse Monoclonal Antibody to NGF Receptor, p75 (CD271) 01464

Publications


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