Y-27632 is a cell-permeable, highly potent and selective inhibitor of Rho-associated, coiled-coil containing protein kinase (ROCK). Y-27632 inhibits both ROCKI (Ki = 220 nM) and ROCKII (Ki = 300 nM) by competing with ATP for binding to the catalytic site. (Davies et al., Ishizaki et al.)
Enhances survival of human embryonic stem (ES) cells when they are dissociated to single cells by preventing dissociation-induced apoptosis (anoikis), thus increasing their cloning efficiency. (Watanabe et al.)
Improves embryoid body formation using forced-aggregation protocols. (Ungrin et al.)
Increases the survival of cryopreserved single human ES cells after thawing. (Li et al.)
Direct lineage reprogramming of fibroblasts to mature neurons, in combination with CHIR99021, Valproic Acid, RepSox, Forskolin, SP600125, and Gö6983 (Hu et al.).