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Embryonic stem cells & induced pluripotent stem (iPS) cells

Undifferentiated pluripotent stem cells have the potential for unlimited expansion with the retention of normal karyotype and the ability to generate cells of all three germ layers. Because of this ability, the use of human embryonic stem cells (ES cells) and human induced pluripotent stem cells (iPS cells) has been proposed in clinical applications, as systems for drug screening and toxicity and as a tool to study cellular and developmental biology. STEMCELL Technologies offers a range of highly specialized cell culture media optimized for culture, differentiation and cryopreservation of ES cells and iPS cells.

Hematopoietic stem & progenitor cells

Hematopoietic cells, including hematopoietic stem cells, can be found in a variety of different tissues through development notably the bone marrow and, to a lesser extent, peripheral blood of adults and the umbilical cord blood earlier in development. Regardless of the location, hematopoietic cells consist of a hierarchy of cells. The hematopoietic stem cell (HSC) is able to differentiate into all terminally differentiated cells found in the blood. Hematopoietic progenitor cells have reduced differentiation and proliferative capacities. STEMCELL Technologies provides a wide variety of media to expand and detect hematopoietic progenitor cells including:

Serum-free hematopoietic expansion media
Methylcellulose-based media for colony-forming unit (CFU) assays
LTC-IC assays to quantitate primitive hematopoietic progenitors

Mesenchymal stem cells

In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. STEMCELL Technologies provides a range of products for the enrichment, quantification, expansion and differentiation of human and mouse mesenchymal stem cells.

Neural stem & progenitor cells

Neural stem cells are defined as cells with the ability to proliferate, self-renew and produce a large number of functional progeny that can differentiate into neurons, astrocytes and oligodendrocytes. STEMCELL Technologies provides a comprehensive range of products for the isolation, maintenance, quantification, expansion and differentiation of neural stem and progenitor cells.

Neurons

Neurons, the functional unit of the central nervous system, are responsible for forming connections and are the communicating cells of the nervous system. STEMCELL Technologies provides serum-free media, supplements and accessory products for the culture and characterization of primary neurons.

Bronchial epithelial cells

The human bronchial epithelium is a ciliated pseudostratified columnar epithelium comprising multiple cell types, including ciliated cells, goblet (mucus-secreting) cells and basal cells. When cultured as a submerged monolayer, bronchial epithelial cells fail to show significant mucociliary differentiation. The air-liquid interface (ALI) culture system has been shown to be a more physiologically relevant culture system because it results in bronchial epithelial cells acquiring a fully differentiated mucociliary phenotype which closely models the human airway. PneumaCult™-ALI is a defined, serum- and BPE-free medium optimized for differentiation of human bronchial epithelial cells cultured at the air-liquid interface.

Mammary epithelial cells

Mammary epithelial cells have been shown to consist of luminal and myoepithelial cells and more recently have been shown to contain stem and progenitor cells capable of replenishing the more differentiated cell types. STEMCELL Technologies offers a variety of specialized and defined media for culture of human and mouse mammary epithelial cells. MammoCult™ is used for the culture of human mammary primary cells and cell lines as mammospheres and tumorspheres. EpiCult™-B cell culture media are used for culture, identification and enumeration of human and mouse mammary epithelial progenitors.

Prostate epithelial cells

There is evidence to suggest that the prostate epithelium is composed of a hierarchy of cells that spans from prostate stem cells to progenitor cells to terminally differentiated cell types. STEMCELL Technologies has developed ProstaCult Medium for the culture of mouse prostate epithelial cells in the prostate colony-forming cell assay (Pr-CFC).

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Product Name	Description	Catalog #	Size	Price
mTeSR™1 Top Seller	Defined, feeder-free maintenance medium for human ESCs and iPSCs	05850	500 mL Kit	272.00 USD
TeSR™-E8™ New Product	Highly defined, low protein, and feeder-free culture medium for maintenance of human ES cells and human iPS cells.	05840	500 mL Kit	196.00 USD
STEMvision™ Instrument	Instrument designed for imaging and scoring of hematopoietic colonies in the colony-forming unit (CFU) assay using MethoCult™ media.	22000	1 Unit	Request pricing
MethoCult™ H4034 Optimum Top Seller	Methylcellulose medium with recombinant cytokines and EPO for human cells	04034	100 mL	418.00 USD
MethoCult™ H4034 Optimum Top Seller	Pre-aliquoted methylcellulose medium with recombinant cytokines and EPO for human cells	04044	24 x 3 mL	515.00 USD
MethoCult™ H4434 Classic	Methylcellulose medium with recombinant cytokines and EPO for human cells	04434	100 mL	418.00 USD
MethoCult™ H4435 Enriched	Methylcellulose medium with recombinant cytokines and EPO for human cells	04435	100 mL	535.00 USD
MethoCult™ Express	Methylcellulose medium for 7-day colony-forming cell assays of human hematopoietic cells	04437	100 mL	628.00 USD
TeSR™2	Animal protein-free maintenance medium for human ES cells and iPS cells	05860	1 Kit	408.00 USD
MethoCult™ GF M3434 Top Seller	Methylcellulose medium with recombinant cytokines and EPO for mouse cells	03434	100 mL	465.00 USD