Specialized cell culture & tissue culture media

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Embryonic stem cells & induced pluripotent stem (iPS) cells

Embryonic Stem Cells
Undifferentiated pluripotent stem cells have the potential for unlimited expansion with the retention of normal karyotype and the ability to generate cells of all three germ layers. Because of this ability, the use of human embryonic stem cells (ES cells) and human induced pluripotent stem cells (iPS cells) has been proposed in clinical applications, as systems for drug screening and toxicity and as a tool to study cellular and developmental biology. STEMCELL Technologies offers a range of highly specialized cell culture media optimized for culture, differentiation and cryopreservation of ES cells and iPS cells.
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Hematopoietic stem & progenitor cells

Hematopoietic Stem Cells
Hematopoietic cells, including hematopoietic stem cells, can be found in a variety of different tissues through development notably the bone marrow and, to a lesser extent, peripheral blood of adults and the umbilical cord blood earlier in development. Regardless of the location, hematopoietic cells consist of a hierarchy of cells. The hematopoietic stem cell (HSC) is able to differentiate into all terminally differentiated cells found in the blood. Hematopoietic progenitor cells have reduced differentiation and proliferative capacities. STEMCELL Technologies provides a wide variety of media to expand and detect hematopoietic progenitor cells including:
  • Serum-free hematopoietic expansion media
  • Methylcellulose-based media for colony-forming unit (CFU) assays
  • LTC-IC assays to quantitate primitive hematopoietic progenitors
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Mesenchymal stem cells

Mesenchymal Stem Cells
In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. STEMCELL Technologies provides a range of products for the enrichment, quantification, expansion and differentiation of human and mouse mesenchymal stem cells.
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Neural stem & progenitor cells

Neural Stem Cells
Neural stem cells are defined as cells with the ability to proliferate, self-renew and produce a large number of functional progeny that can differentiate into neurons, astrocytes and oligodendrocytes. STEMCELL Technologies provides a comprehensive range of products for the isolation, maintenance, quantification, expansion and differentiation of neural stem and progenitor cells.
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Neurons, the functional unit of the central nervous system, are responsible for forming connections and are the communicating cells of the nervous system. STEMCELL Technologies provides serum-free media, supplements and accessory products for the culture and characterization of primary neurons.
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Bronchial epithelial cells

Bronchial epithelial cells cultured at the air-liquid interface with PneumaCult™-ALI closely model the human bronchial epithelium
The human bronchial epithelium is a ciliated pseudostratified columnar epithelium comprising multiple cell types, including ciliated cells, goblet (mucus-secreting) cells and basal cells. When cultured as a submerged monolayer, bronchial epithelial cells fail to show significant mucociliary differentiation. The air-liquid interface (ALI) culture system has been shown to be a more physiologically relevant culture system because it results in bronchial epithelial cells acquiring a fully differentiated mucociliary phenotype which closely models the human airway. PneumaCult™-ALI is a defined, serum- and BPE-free medium optimized for differentiation of human bronchial epithelial cells cultured at the air-liquid interface.
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Mammary epithelial cells

Mammary Epithelial Cells
Mammary epithelial cells have been shown to consist of luminal and myoepithelial cells and more recently have been shown to contain stem and progenitor cells capable of replenishing the more differentiated cell types. STEMCELL Technologies offers a variety of specialized and defined media for culture of human and mouse mammary epithelial cells.  MammoCult™ is used for the culture of human mammary primary cells and cell lines as mammospheres and tumorspheres. EpiCult™-B cell culture media are used for culture, identification and enumeration of human and mouse mammary epithelial progenitors.
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Prostate epithelial cells

Prostate Epithelial Cells
There is evidence to suggest that the prostate epithelium is composed of a hierarchy of cells that spans from prostate stem cells to progenitor cells to terminally differentiated cell types. STEMCELL Technologies has developed ProstaCult Medium for the culture of mouse prostate epithelial cells in the prostate colony-forming cell assay (Pr-CFC).
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170 products available

Product Name Description Catalog # Size Price Quantity
Top Seller
Defined, feeder-free maintenance medium for human ESCs and iPSCs 05850 500 mL Kit Request pricing
TeSR™-E8™ Kit for hESC/hiPSC Maintenance Feeder-free, low protein, culture medium for maintenance of human ES cells and iPS cells 05940 500 mL kit Request pricing
STEMvision™ Instrument Instrument designed for imaging and scoring of hematopoietic colonies in the colony-forming unit (CFU) assay using MethoCult™ media. 22000 1 Unit Request pricing
MethoCult™ H4034 Optimum
Recommended Product
Methylcellulose medium with recombinant cytokines and EPO for human cells 04034 100 mL Request pricing
TeSR™-E7™ Medium for Reprogramming
New Product
Feeder-free reprogramming medium for the generation of human iPS cells 05910 500 mL Request pricing
MethoCult™ H4434 Classic Methylcellulose medium with recombinant cytokines and EPO for human cells 04434 100 mL Request pricing
MethoCult™ H4034 Optimum
Recommended Product
Pre-aliquoted methylcellulose medium with recombinant cytokines and EPO for human cells 04044 24 x 3 mL Request pricing
TeSR™2 Animal protein-free maintenance medium for human ES cells and iPS cells 05860 1 Kit Request pricing
MethoCult™ H4435 Enriched Methylcellulose medium with recombinant cytokines and EPO for human cells 04435 100 mL Request pricing
MethoCult™ Express Methylcellulose medium for 7-day colony-forming cell assays of human hematopoietic cells 04437 100 mL Request pricing

Education Resources


 MethoCult™ for Hematopoietic Colony-Forming Cell Assays
 NeuroCult™-XF: Xeno-Free Culture Medium for the Proliferation of Human Neural Stem Cells
 Products for Human Pluripotent Stem Cells
 MesenCult™-XF: Defined, Xeno-Free Culture of Human Mesenchymal Stem Cells
 Standardized Solutions for Cancer Stem Cell Research
 PneumaCult™ Culture System for Bronchial Epithelial Cells
 NeuroCult™: Reagents for Brain Tumor Stem Cell Research
 Cell Culture Antibodies
 Cell Culture and Immunology Antibodies
 TeSR™-E7™ Reprogramming Medium for Human iPS Cell Induction
 StemSpan™ CD34+ Expansion Supplement
 StemSpan™ Erythroid Expansion Supplement
 StemSpan™ Megakaryocyte Expansion Supplement
 SmartDish™ and STEMgrid™-6 Meniscus Free Cultureware for More Accurate Counting of Hematopoietic Colonies
 Maximize Your Pluripotential with the TeSR™ Family of hPSC Culture Media
 Mesenchymal Stem Cells: MesenCult™: Your High-Performance System for MSC Isolation, Culture & Differentiation
 Easy, Standardized hPSC Differentiation to Definitive Endoderm


 SnapShot: Breast Cancer

Tech bulletin

 Monolayer Culture of Mouse Mammary Epithelial Cells
 Culture of Hematopoietic Stem and Progenitor Cells
 MethoCult™ formulation optimized for mouse BFU-E detection
 MethoCult™��for rat hematopoietic colony assays
 Tumorsphere culture of human breast cancer cell lines
 AT MSCs in MesenCult XF
 Enzyme-free Passaging of Human Embryonic and Induced Pluripotent Stem Cells
 Monolayer Culture of Human Mammary Epithelial Cells
 Air-Liquid Interface Culture for Respiratory Research
 Validation of STEMvision™ for Automated Scoring of Human CFU Assays
 A Sphere Culture Method for Mucociliary Differentiation of Primary Human Bronchial Epithelial Cells

Scientific Poster

 Enhanced Chondrogenic Potential and Immunosuppressive Activity of Human Mesenchymal Progenitor Cells Cultured in a Novel Xeno-Free Culture Medium
 Mammosphere Culture Supports Short But Not Long-Term Propagation of Human Mammary Epithelial Progenitors
 Significantly greater numbers of neurons, neurite outgrowth and branch points in 21 day cultures of primary rat cortical neurons with NeuroCult™ Neuronal Media
 Generation, Maintenance and Cryopreservation of Neural Progenitor Cells Derived from Human Pluripotent Stem Cells Using the STEMdiff™ Neural System
 Induced pluripotent stem cells generated from multiple somatic cell types via feeder free reprogramming in TeSR™-E7™ Medium
 Highly efficient differentiation of human pluripotent stem cells maintained in TeSR™-E8™ to definitive endoderm
 An Animal Component-Free Chondrogenic Stimulatory Medium for the Efficient Differentiation of Human Mesenchymal Progenitor cells
 A novel animal component-free culture medium optimized for derivation and expansion of mesenchymal cells from bone marrow and adipose tissues
 PneumaCult™: An integrated culture medium system for in vitro human airway modeling
 Scalable Enzyme-Free Passaging of Human Pluripotent Stem Cells Cultured in mTeSR™1 or TeSR™-E8™ Without Scraping
 Neural progenitor cells can be generated efficiently using STEMdiff™ Neural Induction Medium by either Embryoid body or Monolayer Culture Methods
 Defined and Xeno-Free Medium for Reprogramming Blood-Derived CD34+ Cells or Erythroid Cells
 Weekend-Free Protocol to Culture Human Pluripotent Stem Cells using mTeSR™1 or TeSR™-E8™
 A novel animal component-free culture medium optimized for derivation and expansion of mesenchymal cells from bone marrow and adipose tissues
 Scalable Passaging of Human Pluripotent Stem Cells Cultured in Defined Media Using ReLeSR™
 Defined, feeder-free human induced pluripotent stem cell (hiPSC) generation, selection and expansion from multiple somatic cell types without manual colony picking or scraping


 In Vitro Hematopoietic Differentiation of mESCs and miPSCs
 Maintenance of mESCs & miPSCs Using ES Cult™
 Maintenance of Human Pluripotent Stem Cells in TeSR™2

Mini Review

 Mini-review: Neural Stem Cells (Catalog #29019)
 Mini-review: Mesenchymal cells (Catalog #29018)
 Mini-review: Mammary Stem Cells (Catalog #29017)
 Mini-review: Hematopoietic Stem and Progenitor Cells (Catalog #29068)