Specialized cell culture & tissue culture media

? 1.800.667.0322   > Contact Us

Embryonic stem cells & induced pluripotent stem (iPS) cells

Embryonic Stem Cells
Undifferentiated pluripotent stem cells have the potential for unlimited expansion with the retention of normal karyotype and the ability to generate cells of all three germ layers. Because of this ability, the use of human embryonic stem cells (ES cells) and human induced pluripotent stem cells (iPS cells) has been proposed in clinical applications, as systems for drug screening and toxicity and as a tool to study cellular and developmental biology. STEMCELL Technologies offers a range of highly specialized cell culture media optimized for culture, differentiation and cryopreservation of ES cells and iPS cells.

Hematopoietic stem & progenitor cells

Hematopoietic Stem Cells
Hematopoietic cells, including hematopoietic stem cells, can be found in a variety of different tissues through development notably the bone marrow and, to a lesser extent, peripheral blood of adults and the umbilical cord blood earlier in development. Regardless of the location, hematopoietic cells consist of a hierarchy of cells. The hematopoietic stem cell (HSC) is able to differentiate into all terminally differentiated cells found in the blood. Hematopoietic progenitor cells have reduced differentiation and proliferative capacities. STEMCELL Technologies provides a wide variety of media to expand and detect hematopoietic progenitor cells including:
  • Serum-free hematopoietic expansion media
  • Methylcellulose-based media for colony-forming unit (CFU) assays
  • LTC-IC assays to quantitate primitive hematopoietic progenitors

Mesenchymal stem cells

Mesenchymal Stem Cells
In culture, mesenchymal stem cells (MSCs) are defined as plastic-adherent, fibroblast-like cells which are able to self-renew and differentiate into bone, adipose and cartilage tissue. STEMCELL Technologies provides a range of products for the enrichment, quantification, expansion and differentiation of human and mouse mesenchymal stem cells.

Neural stem & progenitor cells

Neural Stem Cells
Neural stem cells are defined as cells with the ability to proliferate, self-renew and produce a large number of functional progeny that can differentiate into neurons, astrocytes and oligodendrocytes. STEMCELL Technologies provides a comprehensive range of products for the isolation, maintenance, quantification, expansion and differentiation of neural stem and progenitor cells.

Neurons

Characterization
Neurons, the functional unit of the central nervous system, are responsible for forming connections and are the communicating cells of the nervous system. STEMCELL Technologies provides serum-free media, supplements and accessory products for the culture and characterization of primary neurons.

Bronchial epithelial cells

Bronchial epithelial cells cultured at the air-liquid interface with PneumaCult™-ALI closely model the human bronchial epithelium
The human bronchial epithelium is a ciliated pseudostratified columnar epithelium comprising multiple cell types, including ciliated cells, goblet (mucus-secreting) cells and basal cells. When cultured as a submerged monolayer, bronchial epithelial cells fail to show significant mucociliary differentiation. The air-liquid interface (ALI) culture system has been shown to be a more physiologically relevant culture system because it results in bronchial epithelial cells acquiring a fully differentiated mucociliary phenotype which closely models the human airway. PneumaCult™-ALI is a defined, serum- and BPE-free medium optimized for differentiation of human bronchial epithelial cells cultured at the air-liquid interface.

Mammary epithelial cells

Mammary Epithelial Cells
Mammary epithelial cells have been shown to consist of luminal and myoepithelial cells and more recently have been shown to contain stem and progenitor cells capable of replenishing the more differentiated cell types. STEMCELL Technologies offers a variety of specialized and defined media for culture of human and mouse mammary epithelial cells.  MammoCult™ is used for the culture of human mammary primary cells and cell lines as mammospheres and tumorspheres. EpiCult™-B cell culture media are used for culture, identification and enumeration of human and mouse mammary epithelial progenitors.

Prostate epithelial cells

Prostate Epithelial Cells
There is evidence to suggest that the prostate epithelium is composed of a hierarchy of cells that spans from prostate stem cells to progenitor cells to terminally differentiated cell types. STEMCELL Technologies has developed ProstaCult Medium for the culture of mouse prostate epithelial cells in the prostate colony-forming cell assay (Pr-CFC).

145 products available

Product Name Description Catalog # Size Price Quantity
mTeSR™1
Top Seller
Defined, feeder-free maintenance medium for human ESCs and iPSCs 05850 500 mL Kit 272.00 USD
TeSR™-E8™
New Product
Highly defined, low protein, and feeder-free culture medium for maintenance of human ES cells and human iPS cells. 05840 500 mL Kit 196.00 USD
STEMvision™ Instrument Instrument designed for imaging and scoring of hematopoietic colonies in the colony-forming unit (CFU) assay using MethoCult™ media. 22000 1 Unit Request pricing
MethoCult™ H4034 Optimum
Top Seller
Methylcellulose medium with recombinant cytokines and EPO for human cells 04034 100 mL 418.00 USD
MethoCult™ H4034 Optimum
Top Seller
Pre-aliquoted methylcellulose medium with recombinant cytokines and EPO for human cells 04044 24 x 3 mL 515.00 USD
MethoCult™ H4434 Classic Methylcellulose medium with recombinant cytokines and EPO for human cells 04434 100 mL 418.00 USD
MethoCult™ H4435 Enriched Methylcellulose medium with recombinant cytokines and EPO for human cells 04435 100 mL 535.00 USD
MethoCult™ Express Methylcellulose medium for 7-day colony-forming cell assays of human hematopoietic cells 04437 100 mL 628.00 USD
TeSR™2 Animal protein-free maintenance medium for human ES cells and iPS cells 05860 1 Kit 408.00 USD
MethoCult™ GF M3434
Top Seller
Methylcellulose medium with recombinant cytokines and EPO for mouse cells 03434 100 mL 465.00 USD

Educational Resources

Brochure

 MethoCult™ for Hematopoietic Colony-Forming Cell Assays
 NeuroCult™-XF: Xeno-Free Culture Medium for the Proliferation of Human Neural Stem Cells
 Standardized Solutions for hESC & hiPSC Research
 MesenCult™-XF: Defined, Xeno-Free Culture of Human Mesenchymal Stem Cells
 Standardized Solutions for Cancer Stem Cell Research
 PneumaCult™-ALI for Bronchial Epithelial Cells
 NeuroCult™: Reagents for Brain Tumor Stem Cell Research

Tech bulletin

 Monolayer Culture of Mouse Mammary Epithelial Cells
 Culture of Hematopoietic Stem and Progenitor Cells
 MethoCult™ formulation optimized for mouse BFU-E detection
 MethoCult™ for rat hematopoietic colony assays
 Isolation and characterization of mouse mammary stem cells
 Tumorsphere culture of human breast cancer cell lines
 AT MSCs in MesenCult XF
 Enzyme-free Passaging of Human Embryonic and Induced Pluripotent Stem Cells
 Monolayer Culture of Human Mammary Epithelial Cells
 Air-Liquid Interface Culture for Respiratory Research

Scientific Poster

 Enhanced Chondrogenic Potential and Immunosuppressive Activity of Human Mesenchymal Progenitor Cells Cultured in a Novel Xeno-Free Culture Medium
 Mammosphere Culture Supports Short But Not Long-Term Propagation of Human Mammary Epithelial Progenitors
 mTeSR™1 & TeSR™2:The Advancement of Culture Media for Undifferentiated Pluripotent Stem Cells Towards Greater Regulatory Compliance

Manual

 In Vitro Hematopoietic Differentiation of mESCs and miPSCs
 Maintenance of mESCs & miPSCs Using ES Cult™
 ClonaCell™ EasyPick Method: Supplementary Manual for Mammalian Colony Picking

Mini Review

 Mini-review: Pluripotent Stem Cells (Catalog #29143)
 Mini-review: Neural Stem Cells (Catalog #29019)
 Mini-review: Mesenchymal cells (Catalog #29018)
 Mini-review: Mammary Stem Cells (Catalog #29017)
 Mini-review: Hematopoietic Stem and Progenitor Cells (Catalog #29784)