RosetteSep™

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 Fast and Easy Cell Isolation Directly From Whole Blood in As  Little As 25 Minutes
RosetteSep™: Fast and Easy Cell Separation Directly From Whole Blood
Request a sample of RosetteSep™ to get untouched human cells without columns or magnets.
The RosetteSep™ and SepMate™ system is an innovative platform for rapid and consistent cell isolation directly from whole blood in just 25 minutes. The system combines a unique immunodensity cell isolation reagent (RosetteSep™) with a specialized cell processing tube (SepMate™) to minimize variability between users and allow efficient, high-throughput sample processing. This new method isolates untouched and highly purified cells in a single step without columns or magnets, helping to avoid the risk of activating or damaging cells.

Cells isolated using the RosetteSep™ and SepMate™ system are immediately available for downstream use, and are ideal for cytotoxicity assays, compound screening, and other high-throughput applications that require viable, functional cells with minimal manipulation.

To learn how one lab used this new system to reduce their NK cell isolation time from 4 hours to 1 hour for a 450 mL unit of blood, read the case study.

Advantages of RosetteSep™


  • Fast and Easy
    Isolate cells from whole blood in as little as 25 minutes with RosetteSep™ and SepMate™, or use RosetteSep™ on its own with standard density gradient centrifugation.

  • No Special Training or Equipment
    RosetteSep™ and SepMate™ can be used by anyone with minimal training. No columns, magnets or other special equipment are required.

  • Highly Viable and Functional Cells
    Isolated cells are functional, flow cytometry-compatible, and unlabeled with antibodies or magnetic beads.

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Figure 1. Image of a blood sample after addition of the RosetteSep™ Cocktail, and prior to density gradient centrifugation
Figure 1. Image of a blood sample after addition of the RosetteSep™ Cocktail, and prior to density gradient centrifugation

How RosetteSep™ Works

To isolate immune cells from whole blood, simply incubate your sample with the appropriate RosetteSep™ antibody cocktail prior to SepMate™ separation or standard density gradient centrifugation. RosetteSep™ crosslinks unwanted cells to red blood cells, forming immunorosettes (Figure 1). These immunorosettes pellet during centrifugation, leaving untouched, highly purified target cells at the interface between the plasma and the density gradient medium. If you are using SepMate™, simply pour purified cells into a new tube. Otherwise, harvest them from the plasma:density gradient medium interface.

For more information on how to use SepMate™ and RosetteSep™, view this short technical video.

The Novel RosetteSep™ and SepMate™ Procedure


Incubate your blood samples with RosetteSep™ for 10 minutes. Rapidly load samples into SepMate™ tubes. The unique SepMate™ insert prevents the blood from mixing with the density gradient medium. After a 10-minute centrifugation with the brake on, simply pour highly purified target cells into a new tube.

68 products available

Product Name Description Catalog # Size Price Quantity
RosetteSep™ Human CD4+ T Cell Enrichment Cocktail Immunodensity isolation of untouched CD4+ T cells 15022 For labeling 40 mL of whole blood 153.00 USD
RosetteSep™ Human T Cell Enrichment Cocktail Immunodensity isolation of untouched T cells 15021 For labeling 40 mL of whole blood 153.00 USD
RosetteSep™ Human CD8+ T Cell Enrichment Cocktail Immunodensity isolation of untouched CD8+ T cells 15023 For labeling 40 mL of whole blood 153.00 USD
RosetteSep™ Human B Cell Enrichment Cocktail Immunodensity isolation of untouched B cells 15024 For labeling 40 mL of whole blood 153.00 USD
RosetteSep™ Human NK Cell Enrichment Cocktail Immunodensity isolation of untouched NK cells 15025 For labeling 40 mL of whole blood 153.00 USD
SepMate™-50
New Product
50 mL centrifuge tube with an insert for density gradient medium-based cell isolation 15450 20 tubes 90.00 USD
RosetteSep™ Human Circulating Epithelial Tumor Cell Enrichment Cocktail Immunodensity isolation of untouched circulating epithelial tumor cells 15127 For labeling 40 mL of whole blood 355.00 USD
RosetteSep™ Human Multiple Myeloma Cell Enrichment Cocktail Immunodensity isolation of untouched plasma cells for multiple myeloma research 15129 For labeling 40 mL of bone marrow 390.00 USD
Complete Kit for CD4+CD25+ T Cells Complete kit for human CD4+CD25+ regulatory T cells 15862 For labeling 200 mL of whole blood 707.00 USD
RosetteSep™ Human Total Lymphocyte Enrichment Cocktail Immunodensity isolation of untouched lymphocytes 15223 For labeling 40 mL of whole blood 153.00 USD