RosetteSep®

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RosetteSep^®is a rapid cell separation method for the isolation of highly purified cells directly from human whole blood. Using Tetrameric Antibody Complexes (TAC), RosetteSep^® turns a simple density centrifugation step into a specific antibody-mediated cell enrichment procedure. The RosetteSep^® cocktail links unwanted cells to multiple red blood cells already present in the sample, forming immunorosettes. When centrifuged over a density medium such as Ficoll-Paque™, the unwanted cells pellet along with the red blood cells, leaving the desired cells untouched and highly enriched at the density medium: plasma interface. Desired cells are never labeled with antibody and are immediately ready for any application.

ONE STEP CELL ENRICHMENT FROM WHOLE BLOOD
Just a 20 minute incubation prior to a standard Ficoll™ spin without the use of columns or magnets.

HIGH PURITY AND RECOVERY
Purities >90% with no post-Ficoll™ cell losses.

UNTOUCHED CELLS
Cells are unlabeled and ready for immediate use.

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Product Name	Description	Catalog #	Size	Price
Complete Kit for CD4+CD127lowCD25+ Regulatory T Cells	Complete kit for human CD4+CD127lowCD25+ regulatory T cells	15861	For labeling 200 mL of whole blood	698.00 USD
Complete Kit for CD4+CD25+ T Cells	Complete kit for human CD4+CD25+ regulatory T cells	15862	For labeling 200 mL of whole blood	647.00 USD
Complete Kit for Human CD4+CD127lowCD49d-CD25+ Regulatory T Cells	Complete kit for human CD4+CD127lowCD49d-CD25+ regulatory T cells	15864	For labeling 200 mL of whole blood	698.00 USD
RosetteSep® Anti-Human IgE Tetramer	Immunodensity depletion of IgE+ cells	15230	For labeling 40 mL of whole blood	99.00 USD
RosetteSep® DM-L Density Medium	Density gradient medium for use with RosetteSep® CD3+ T cell, CD4+ T cell, CD8+ T cell, and B Cell enrichment cocktails	15705	100 mL	33.00 USD
RosetteSep® DM-M Density Medium	Density gradient medium	15725	100 mL	39.00 USD
RosetteSep® HLA B Cell Enrichment Cocktail	Immunodensity isolation of untouched B cells for HLA analysis	15064HLA	For labeling 250 mL of blood	526.00 USD
RosetteSep® HLA B Cell Enrichment Cocktail	Immunodensity isolation of untouched B cells for HLA analysis	15084HLA	For labeling 1000 mL of blood	1895.00 USD
RosetteSep® HLA Granulocyte Depletion Cocktail	Immunodensity depletion of granulocytes for HLA analysis	15664HLA	For labeling 250 mL of whole blood	467.00 USD
RosetteSep® HLA Granulocyte Depletion Cocktail	Immunodensity depletion of granulocytes for HLA analysis	15684HLA	For labeling 1000 mL of whole blood	1540.00 USD