MesenCult™ Mesenchymal Stem Cell Stimulatory Supplements (Human)

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Supplement for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells

  • Mesenchymal Stem Cell Stimulatory Supplements (Human) 50 mL
  • Label for Mesenchymal Stem Cell Stimulatory Supplements (Human) 50 mL
Mesenchymal Stem Cell Stimulatory Supplements (Human) 50 mL
MesenCult™ Mesenchymal Stem Cell Stimulatory Supplements (Human) is a standardized, serum-containing supplement for the culture of human mesenchymal stem cells (MSCs). The MesenCult™ Mesenchymal Stem Cell Stimulatory Supplements (Human) is optimized for the expansion of human mesenchymal stem cells in vitro as well as their enumeration using the colony-forming unit - fibroblast (CFU-F) assay when combined with MesenCult™ MSC Basal Medium (Human). Components are prescreened to minimize lot-to-lot variability. This supplement is also a component of the MesenCult™ Proliferation Kit (Human; Catalog #05411).
Product Name Description Catalog # Size Price Quantity
MesenCult™ Stimulatory Supplements (Human) Supplement for detection of CFU-F and expansion of human mesenchymal stem cells 05402 50 mL 132.00 USD      
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Application: Colony assays & quantification, Expansion
Product Type: Specialized cell culture media
Recommended For:
• Expansion of human mesenchymal stem cells when combined with MesenCult™ MSC Basal Medium (Human; Catalog #05401)
• Enumeration of human mesenchymal stem cells using the CFU-F assay when combined with MesenCult™ MSC Basal Medium (Human; Catalog #05401)
 
Components:
N/A
Accessory Products:
• MesenCult™ MSC Basal Medium (Human; Catalog #05401)
• MesenCult™ Proliferation Kit (Human; Catalog #05411)
• MesenCult™ Osteogenic Stimulatory Kit (Human; Catalog #05404)
• EasySep™ Human CD271 Positive Selection Kit (Catalog #18659)
• RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail (Catalog #15128/15168)
Contains:
N/A
Area of Interest: Mesenchymal stem cell research, Stem cell biology
Cell Type: Mesenchymal stem cells
Medium Type: Liquid
Popular Product Line: MesenCult™
Species: Human
Legal Statement:
N/A
Intended Use Statement: FOR RESEARCH USE ONLY. NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES.
ISO Statement: STEMCELL TECHNOLOGIES INC.’S QUALITY MANAGEMENT SYSTEM IS CERTIFIED TO ISO 13485 MEDICAL DEVICE STANDARDS.

Procedures and instruction manuals:

Educational resources:

MSDS:

This product has been used in:

  1. Issam Hussain et al. New Approach for Isolation of Mesenchymal Stem Cells (MSCs) From Human Umbilical Cord Blood.Cell Biol Int (February 17, 2012)
  2. Carmen Mariana Aanei et al. Focal adhesion protein abnormalities in myelodysplastic mesenchymal stromal cells.Exp Cell Res 317 (18) 2616-2629 (November 1, 2011)
  3. Avital Mendelson et al. Chondrogenesis by chemotactic homing of synovium, bone marrow, and adipose stem cells in vitro.FASEB J 25 (10) 3496-3504 (October 2011)
  4. Elisa Ciraci et al. Adult human circulating CD34???Lin???CD45???CD133??? cells can differentiate into hematopoietic and endothelial cells.Blood 118 (8) 2105-2115 (June 29, 2011)
  5. Antony J Cutler et al. Umbilical cord-derived mesenchymal stromal cells modulate monocyte function to suppress T cell proliferation.J Immunol 185 (11) 6617-6623 (December 1, 2010)
  6. Dirk Henrich et al. RIA reamings and hip aspirate: a comparative evaluation of osteoprogenitor and endothelial progenitor cells.Injury 41 Suppl 2 S62-8 (November 2010)
  7. Rosa Ya??ez et al. Prostaglandin E2 plays a key role in the immunosuppressive properties of adipose and bone marrow tissue-derived mesenchymal stromal cells.Exp Cell Res 316 (19) 3109-3123 (November 15, 2010)
  8. Caroline Seebach et al. Comparison of six bone-graft substitutes regarding to cell seeding efficiency, metabolism and growth behaviour of human mesenchymal stem cells (MSC) in vitro.Injury 41 (7) 731-738 (July 2010)
  9. Li-Shan Wang et al. Injectable biodegradable hydrogels with tunable mechanical properties for the stimulation of neurogenesic differentiation of human mesenchymal stem cells in 3D culture.Biomaterials 31 (6) 1148-1157 (February 2010)
  10. Henry L Chang et al. Uterine leiomyomas exhibit fewer stem/progenitor cell characteristics when compared with corresponding normal myometrium.Reprod Sci 17 (2) 158-167 (February 2010)
  11. Simon C Pitchford et al. Troubleshooting: Quantification of mobilization of progenitor cell subsets from bone marrow in vivo.J Pharmacol Toxicol Methods 61 (2) 113-121 (February 4, 2010)
  12. Kim Chi T Bui et al. Recovery of multipotent progenitors from the peripheral blood of patients requiring extracorporeal membrane oxygenation support.Am J Respir Crit Care Med 181 (3) 226-237 (February 1, 2010)
  13. Pablo Menendez et al. Bone marrow mesenchymal stem cells from infants with MLL-AF4+ acute leukemia harbor and express the MLL-AF4 fusion gene.J Exp Med 206 (13) 3131-3141 (December 21, 2009)
  14. Florian H Seeger et al. CXCR4 expression determines functional activity of bone marrow-derived mononuclear cells for therapeutic neovascularization in acute ischemia.Arterioscler Thromb Vasc Biol 29 (11) 1802-1809 (November 2009)
  15. Erik J Woods et al. Optimized cryopreservation method for human dental pulp-derived stem cells and their tissues of origin for banking and clinical use.Cryobiology 59 (2) 150-157 (October 2009)
  16. Yongdong Feng et al. Unique biomechanical interactions between myeloma cells and bone marrow stroma cells.Prog Biophys Mol Biol (October 17, 2009)
  17. Catherine A Cremona et al. Loss of anchorage in checkpoint-deficient cells increases genomic instability and promotes oncogenic transformation.J Cell Sci 122 (Pt 18) 3272-3281 (September 15, 2009)
  18. Brandon C Perry et al. Collection, cryopreservation, and characterization of human dental pulp-derived mesenchymal stem cells for banking and clinical use.Tissue Eng Part C Methods 14 (2) 149-156 (June 2008)
  19. Nadia Sessarego et al. Multipotent mesenchymal stromal cells from amniotic fluid: solid perspectives for clinical application.Haematologica 93 (3) 339-346 (March 2008)
  20. Activation of Epac stimulates integrin-dependent homing of progenitor cells.Blood 111 (5) 2640-2646 (March 1, 2008)
  21. Elisabetta Traggiai et al. Bone marrow-derived mesenchymal stem cells induce both polyclonal expansion and differentiation of B cells isolated from healthy donors and systemic lupus erythematosus patients.Stem Cells 26 (2) 562-569 (February 2008)
  22. Grazia Maria Spaggiari et al. Mesenchymal stem cells inhibit natural killer-cell proliferation, cytotoxicity, and cytokine production: role of indoleamine 2,3-dioxygenase and prostaglandin E2.Blood 111 (3) 1327-1333 (February 1, 2008)
  23. Lizzia Raffaghello et al. Human mesenchymal stem cells inhibit neutrophil apoptosis: a model for neutrophil preservation in the bone marrow niche.Stem Cells 26 (1) 151-162 (January 1, 2008)
  24. Antonio P Beltrami et al. Multipotent cells can be generated in vitro from several adult human organs (heart, liver, and bone marrow).Blood 110 (9) 3438-3446 (November 1, 2007)
  25. Wolfgang Wagner et al. Molecular and secretory profiles of human mesenchymal stromal cells and their abilities to maintain primitive hematopoietic progenitors.Stem Cells 25 (10) 2638-2647 (October 2007)
  26. Federica Benvenuto et al. Human mesenchymal stem cells promote survival of T cells in a quiescent state.Stem Cells 25 (7) 1753-1760 (July 2007)
  27. Rosa Yanez et al. Adipose tissue-derived mesenchymal stem cells have in vivo immunosuppressive properties applicable for the control of the graft-versus-host disease.Stem cells (Dayton, Ohio) 24 (11) 2582-2591 (November 2006)
  28. Sara Pozzi et al. Donor multipotent mesenchymal stromal cells may engraft in pediatric patients given either cord blood or bone marrow transplantation.Exp Hematol 34 (7) 934-942 (July 2006)
  29. M Jager et al. Extensive H(+) release by bone substitutes affects biocompatibility in vitro testing.Journal of biomedical materials research. Part A 76 (2) 310-322 (February 2006)
  30. Grazia Maria Spaggiari et al. Mesenchymal stem cell-natural killer cell interactions: evidence that activated NK cells are capable of killing MSCs, whereas MSCs can inhibit IL-2-induced NK-cell proliferation.Blood 107 (4) 1484-1490 (February 15, 2006)
  31. Anna Corcione et al. Human mesenchymal stem cells modulate B-cell functions.Blood 107 (1) 367-372 (January 1, 2006)
  32. Andrea Bacigalupo et al. T-cell suppression mediated by mesenchymal stem cells is deficient in patients with severe aplastic anemia.Exp Hematol 33 (7) 819-827 (July 2005)
  33. M. Mattioli-Belmonte et al. Mesenchymal Stem Cells on Plasma-Deposited Acrylic Acid Coatings: An In Vitro Investigation to Improve Biomaterial Performance in Bone ReconstructionJournal of Bioactive and Compatible Polymers 20 (4) 343-360 (July 1, 2005)
  34. C G Steward et al. High peripheral blood progenitor cell counts enable autologous backup before stem cell transplantation for malignant infantile osteopetrosis.Biol Blood Marrow Transplant 11 (2) 115-121 (February 2005)
  35. Libuse Tauchmanova et al. Short-term zoledronic acid treatment increases bone mineral density and marrow clonogenic fibroblast progenitors after allogeneic stem cell transplantation.The Journal of clinical endocrinology and metabolism 90 (2) 627-634 (February 2005)
  36. Karen Bieback et al. Critical parameters for the isolation of mesenchymal stem cells from umbilical cord blood.Stem Cells 22 (4) 625-634 (2004)
  37. Norbert Ahrens et al. Mesenchymal stem cell content of human vertebral bone marrow.Transplantation 78 (6) 925-929 (September 27, 2004)
  38. Laurence Vaysse et al. Development of a self-assembling nuclear targeting vector system based on the tetracycline repressor protein.J Biol Chem 279 (7) 5555-5564 (February 13, 2004)
  39. Libuse Tauchmanova et al. Avascular necrosis in long-term survivors after allogeneic or autologous stem cell transplantation: a single center experience and a review.Cancer 97 (10) 2453-2461 (May 15, 2003)
  40. Anna Merino et al. Transfusion medicine illustrated. The mesenchymal stem cell revealed.Transfusion 43 (1) 1 (January 2003)

Background References:

  1. M Dominici et al. Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement.Cytotherapy 8 (4) 315-317 (2006)

Product Name

Description

Catalog #

MesenCult™ MSC Basal Medium (Human) Basal Medium for Human Mesenchymal Stem Cells 05401
MesenCult™ Proliferation Kit (Human) Medium for Detection of CFU-F and Expansion of Human Mesenchymal Stem Cells 05411
MesenCult™ Osteogenic Stimulatory Kit (Human) Supplement for Differentiating Human Mesenchymal Stem Cells into Osteogenic Progenitors 05404
MesenCult™-XF Medium Defined, Xeno-Free Medium for Human Mesenchymal Stem Cells 05420
MesenCult™-SF Culture Kit Defined, Serum-Free Culture Kit for Human Mesenchymal Stem Cells 05429
RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail Immunodensity Negative Selection Cocktail 15128
RosetteSep™ Human Mesenchymal Stem Cell Enrichment Cocktail 15168
EasySep™ Human CD271 Selection Kit Immunomagnetic Positive Selection Kit 18659

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