NeuroCult® NS-A Differentiation Kit (Human)

Recommended for:	In vitro differentiation of human neural stem and progenitor cells into neurons, astrocytes and oligodendrocytes
Components:	• NeuroCult® NS-A Basal Medium (Human), 450 mL (Catalog #05750) • NeuroCult® NS-A Differentiation Supplement (Human), 50 mL (Catalog #05754)
Intended Use Statement:	For Research Use Only. Not for Therapeutic or Diagnostic Use.
Contains:	N/A
Legal Statement:	Sold under license from StemCells California, Inc. US Patent Nos. 5,750,376; 5,851,832; 5,980,885; 5,968,829; 5,981,165; 6,071,889; 6,093,531; 6,103,530; 6,165,783; 6,238,922.
Product Type:	Specialized cell culture media
Application:	Differentiation
Area of Interest:	Cancer, Neuroscience, Stem cell biology
Cell Type:	Neural stem & progenitor cells
Medium Type:	Liquid
Popular Product Line:	NeuroCult
Species:	Human

Procedures and instruction manuals:

•  MANUAL: In Vitro Proliferation and Differentiation of Human Neural Stem and Progenitor Cells and Progenitor Cells using NeuroCult™ or NeuroCult™-XF
•  PRODUCT INFORMATION SHEET - 05752, Lot# All:29580-PIS_1_1_0.pdf

Educational resources:

•  WEBINAR: Standardized Media and Reagents for Neural Stem Cell Research with NeuroCult®
•  BROCHURE: Neural Stem Cells: Standardized Media and Reagents
•  MINI REVIEW: Mini-review: Neural stem cells (Catalog #29019)
•  BROCHURE: NeuroCult™ Reagents for Brain Tumor Stem Cell Research
•  WEBINAR: Identification and Characterization of Neural Stem Cells: Why is This so Darned Difficult?

MSDS:

•  05754-MSDS.pdf

Product Name	Description	Catalog #
NeuroCult® NS-A Proliferation Kit (Human)	Medium for Expansion of Human Neural Stem and Progenitor Cells	05751
ALDEFLUOR™ Kit	ALDEFLUOR™ Kit for the Identification, Evaluation and Isolation of Stem and Progenitor Cells Expressing High Levels of ALDH	01700
NeuroCult®-XF Proliferation Medium	Xeno-Free Medium for Expansion of Human Neural Stem and Progenitor Cells	05761
NeuroCult® Neural Stem Cell Functional Identification Kit	Antibody-Based Neural Cell Identification Kit	05716

Immunofluorescent staining to identify the differentiated cell types generated following culture of neural stem and progenitor cells in NeuroCult® NS-A Differentiation Medium

A) Neurons (red) were detected with a mouse monoclonal ß-Tubulin III antibody.
B) Immature oligodendrocytes (purple) were detected with a rabbit monoclonal O4 Oligodendrocyte Marker antibody.
C) Astrocytes (green) were detected with a rabbit polyclonal GFAP antibody.
D) Mature oligodendrocytes (purple) were detected with a galactocerebroside antibody.