EasySep® Human CD8+ T Cell Enrichment Kit

Procedures and instruction manuals:

•  PRODUCT INFORMATION SHEET - 19053, Lot# 10J36954 or less:28743-PIS_3_0_1.pdf
•  PRODUCT INFORMATION SHEET - 19053, Lot# 11B38234 or greater:28743-PIS_4_1_0.pdf
•  PRODUCT INFORMATION SHEET - 19053, Lot# 11B38234 or greater:28743-Easy50-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19053, Lot# 11B38234 or greater:28743-EasyPlate-PIS_1_0_0.pdf
•  PRODUCT INFORMATION SHEET - 19053RF, Lot# 10J36954 or less:28743-PIS_3_0_1.pdf
•  PRODUCT INFORMATION SHEET - 19053RF, Lot# 11B38234 or greater:28743-PIS_4_1_0.pdf

Educational resources:

•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: The EasySep® Pour-off: A Simple and Effective Way to Isolate Cells
•  VIDEO: EasySep®: Powerful Immunomagnetic Isolation of Virtually Any Cell Type
•  VIDEO: HetaSep™: A Guide to Isolating Nucleated Cells from Whole Blood
•  BROCHURE: RoboSep®: Solutions for HIV Research
•  BROCHURE: Cell Separation Equipment & Products
•  BROCHURE: Cell Separation for Autoimmunity Research
•  WALLCHART: Antigen Processing and Presentation Wallchart
•  BROCHURE: Ready Sep Go – Cell Separation In As Little As 25 Minutes
•  VIDEO: The EasySep® MultiStand: Four Samples, Four Separations, One Pour-off
•  BROCHURE: Customized Cell Isolation: Flexible Cell Isolation Designed for you and your Research
•  VIDEO: RoboSep® Product Tour
•  VIDEO: How to Isolate Mononuclear Cells from Whole Blood by Density Gradient Centrifugation
•  VIDEO: RoboSep® Sneak Preview

MSDS:

•  19013C.2_19053C.2-MSDS.pdf
•  19250_19250H-MSDS.pdf

FAQS:

• 
  HOW EASYSEP™ WORKS
  
  
  • Q. CAN EASYSEP™ BE USED FOR EITHER POSITIVE OR NEGATIVE SELECTION?
    A. Yes. The EasySep™ kits use either a negative selection approach by targeting and removing unwanted cells or a positive selection approach targeting desired cells. Depletion kits are also available for the removal of cells with a specific undesired marker (e.g. GlyA).
  • Q. HOW DOES THE SEPARATION WORK?
    A. Magnetic particles are crosslinked to cells using Tetrameric Antibody Complexes (TAC). When placed in the EasySep™ Magnet, labeled cells migrate to the wall of the tube. The unlabeled cells are then poured off into a separate fraction.
  • Q. WHICH COLUMNS DO I USE?
    A. The EasySep™ procedure is column-free. That's right - no columns!
  • Q. HOW CAN I ANALYZE THE PURITY OF MY ENRICHED SAMPLE?
    A. The Product Information Sheet provided with each EasySep™ kit contains detailed staining information.
  • Q. CAN EASYSEP™ SEPARATIONS BE AUTOMATED?
    A. Yes. RoboSep™, the fully automated cell separator, automates all EasySep™ labeling and cell separation steps.
  • Q. CAN EASYSEP™ BE USED TO ISOLATE RARE CELLS?
    A. Yes. We recommend a cell concentration of 2x10⁸ cells/mL and a minimum working volume of 100 µL. Samples containing 2x10⁷ cells or fewer should be suspended in 100 µL of buffer.
  
  EASYSEP™ MAGNETIC PARTICLES
  
  
  • Q. ARE THE EASYSEP™ MAGNETIC PARTICLES FACS-COMPATIBLE?
    A. Yes, the EasySep™ particles are flow cytometry-compatible, as they are very uniform in size and about 5000X smaller than other commercially available magnetic beads used with column-free systems.
  • Q. CAN THE EASYSEP™ MAGNETIC PARTICLES BE REMOVED AFTER ENRICHMENT?
    A. No, but due to the small size of these particles, they will not interfere with downstream applications.
  • Q. HOW DOES THE BINDING OF THE EASYSEP™ MAGNETIC PARTICLE AFFECT THE CELLS? IS THE FUNCTION OF POSITIVELY SELECTED CELLS ALTERED BY THE BOUND PARTICLES?
    A. Hundreds of publications have used cells selected with EasySep™ positive selection kits for functional studies. Our in-house experiments also confirm that selected cells are not functionally altered by the EasySep™ magnetic particles.
    
    If particle binding is a key concern, we offer two options for negative selection. The EasySep™ negative selection kits can isolate untouched cells with comparable purities, while RosetteSep™ can isolate untouched cells directly from whole blood without using particles or magnets.
  
  MODIFYING THE EASYSEP™ PROTOCOL
  
  
  • Q. CAN I ALTER THE SEPARATION TIME IN THE MAGNET?
    A. Yes; however, this may impact the kit's performance. The provided EasySep™ protocols have already been optimized to balance purity, recovery and time spent on the isolation.
  • Q. FOR POSITIVE SELECTION, CAN I PERFORM MORE THAN 3 SEPARATIONS TO INCREASE PURITY?
    A. Yes, the purity of targeted cells will increase with additional rounds of separations; however, cell recovery will decrease.

This product has been used in:

1. Stanley T Parish et al. Adenosine deaminase modulation of telomerase activity and replicative senescence in human CD8 T lymphocytes.J Immunol 184 (6) 2847-2854 (March 15, 2010)
2. Eva Billerbeck et al. Analysis of CD161 expression on human CD8+ T cells defines a distinct functional subset with tissue-homing properties.Proc Natl Acad Sci U S A 107 (7) 3006-3011 (February 16, 2010)
3. Patrizia Fuschiotti et al. Effector CD8+ T cells in systemic sclerosis patients produce abnormally high levels of interleukin-13 associated with increased skin fibrosis.Arthritis Rheum 60 (4) 1119-1128 (April 2009)
4. Brenna C Simons et al. Despite biased TRBV gene usage against a dominant HLA B57-restricted epitope, TCR diversity can provide recognition of circulating epitope variants.J Immunol 181 (7) 5137-5146 (October 1, 2008)
5. Angela J Fahey et al. Reciprocal effects of IFN-beta and IL-12 on STAT4 activation and cytokine induction in T cells.J Leukoc Biol 81 (6) 1562-1567 (June 2007)
6. Lesley White et al. Differential effects of IL-21 and IL-15 on perforin expression, lysosomal degranulation, and proliferation in CD8 T cells of patients with human immunodeficiency virus-1 (HIV).Blood 109 (9) 3873-3880 (May 1, 2007)

FACS Histogram Results Using EasySep® Human CD8+ T Cell Enrichment Kit

Typical EasySep® Procedure for Human Cells