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Ready Sep Go

with RoboSep™-S
Fully Automated, Walk-Away Cell Isolation

Streamline your cell isolations using the fully automated RoboSep™-S cell isolation platform. RoboSep™-S, the updated and improved version of the popular RoboSep™ instrument, offers true walk-away automation while retaining the speed and simplicity of EasySep™ with just 5 minutes of hands-on time.

Automated cell isolation using RoboSep™-S is:

FAST AND EASY. Isolated cells are immediately ready for flow cytometry, functional studies or other downstream applications.
VERSATILE. Virtually any cell type can be isolated from a wide range of sample sources and sizes (e.g. whole blood, leukopaks, PBMCs, splenocytes) using positive or negative selection protocols.
SAFE. With a column-free design and minimal sample handling required, the risk of sample cross-contamination and the risk of exposure to dangerous pathogens is minimized.
HIGHLY CUSTOMIZABLE. For special or non-standard applications, our experts can work with you to design customized cell separation protocols to isolate cells of interest.

To view cell isolation kits for use with RoboSep™-S, click here.
Explore Tools for Automated Cell Isolation
RoboSep™-S       |       Featured Applications       |       Educational Resources

Product Tour

By combining an exceptionally compact design, a simple, user-friendly interface and the fast and easy cell separation protocols you’ve come to expect from STEMCELL Technologies, RoboSep™-S fits easily into the workflow of any lab that needs the multi-sample processing capacity, speed, reliability and convenience of automated cell isolation.



Compact Design No Daily Maintenance

Exceptionally small footprint (42 cm x 42 cm) frees up valuable lab space
Place multiple units side-by-side in biological safety cabinets or on lab benches

No daily cleaning, washing, decontamination, etc. is required
Hydraulic fluid levels are automatically monitored and users are notified when refilling is required

Minimal Sample Handling with No Cross-Contamination Simultaneous or Sequential Cell Isolation
Automated sample handling reduces the risk of exposure to dangerous pathogens when handling biological samples (Case Study: HIV Research Profiles - How Ex Vivo Models Drive Progress in HIV Research)
Disposable tips and a column-free system eliminate the risk of sample cross-contamination

Simultaneous isolations from up to 4 samples at once
Sequential isolation of different cell types from the same sample
(Case Study: Fully Automated Sequential Cell Isolation of Four Different Cell Types from a Single Sample with RoboSep™)

Reliable and Consistent Performance Simple Reagent and Experiment Tracking
With RoboSep™-S, cell separation results are reliable and reproducible, with minimal inter-user variability

Integrated barcode reader allows for reliable reagent tracking
Simple End-of-Run reports include user and protocol details as well as reagent information

Minimal Downtime Quick Start Options
Comprehensive instrument service packages minimize unplanned downtime that can negatively impact lab efficiency
Our Technical Support experts can quickly access diagnostic packages and video logs to assist with troubleshooting

Start a cell separation protocol in just 5 minutes with minimal touchscreen navigation
Use pre-programmed user profiles or custom profiles with commonly run protocols

Highly Purified Cells Flexible Financing Options

Isolated cells are highly purified (up to 99%)
Cells are immediately ready for flow cytometry, functional studies or other downstream applications

RoboSep™-S is available for purchase or lease
Please contact us for more information

Featured Applications

Immunology and Infectious Disease Research
RoboSep™ minimizes the risk associated with handling hazardous biological specimens and has been used to support studies on:
Pathogens including HIV, other viruses and bacteria1-9
(Case Study: HIV Research Profiles - How Ex Vivo Models Drive Progress in HIV Research)
Autoimmune diseases including diabetes and rheumatoid arthritis10-15
Transplantation immunology16-22

Hematological Malignancies
RoboSep™ effectively enriches rare cell types to enhance assay sensitivity for research on:
Multiple Myeloma26-32
Chronic Lymphocytic Leukemia33-38
Acute Myeloid Leukemia39-40
Myelodysplastic Syndrome41-44

Chimerism and HLA Testing
RoboSep™ facilitates simultaneous or sequential cell isolation with reliable and reproducible results for:
Chimerism analysis45,46
(Case Study: Fully Automated Sequential Cell Isolation of Four Different Cell Types from a Single Sample with RoboSep™)
Flow Cytometry Crossmatch (FCXM)
Serology-based assays

Assay Development and Immunotherapy Research
RoboSep™ has been used to isolate highly purified, functional cells from a variety of sample sources for:
Drug discovery and development15,47-49
Immune cell-based assay development
Vaccine development29
Basic cellular therapy research50

Educational Resources

RoboSep™-S Introduction
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™

HIV Research Profiles
How Ex Vivo Models Drive Progress in HIV Research
Technical Bulletin
Enhance Sensitivity of FISH Analysis with Purified Multiple Myeloma Cells

Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Frequencies of Cell Types in Normal Human Peripheral Blood

1. Miles B et al. J Leuk Biol 94(2):281-289, 2013
2. Prince LR et al. PLoS One 7(2):e31506, 2012
3. Cohen S et al. JAMA 309(7):699-705, 2013
4. Wilen CB et al. PLoS Pathog 7(4):e1002020, 2011
5. Sáez-Cirión A et al. Nat Protoc 5:1033-1041, 2010
6. Sáez-Cirión A et al. Blood 118(4):955-964, 2011
7. Ndhlovu ZM et al. J Virol 86(12):6959-6969, 2012
8. Vetter M et al. J Virol 83(17):8646-8654, 2009
9. Chun T-W et al. AIDS 24(18):2803-2808, 2010
10. Martinez-Llordella M et al. J Exp Med 210(8):1603-1619, 2013
11. Montandon R et al. PNAS 110(24):E2199-2208, 2013
12. Esensten JH et al. J Immunol 183(1):75-82, 2009
13. O’Boyle G et al. PNAS 109(12):4598-4603, 2012
14. Karnell JL et al. J Immunol 187(7):3603-3612, 2011
15. Durand CA et al. Autoimmunity 46(1):62-73, 2013
16. Haniffa M et al. J Exp Med 206(2):371-385, 2009
17. Costa C et al. Trans Proceed 43(4):1159-1161, 2011
18. Mavin E et al. Transplantation 94(5):456-464, 2012
19. Kirkiles-Smith NC et al. Transplantation 87(2):189-197, 2009
20. Søndergaard HS et al. Clin Exp Immunol 172(2):300-310, 2013
21. Wang X-N et al. Transplantation 88(2):188-197, 2009
22. Hüser N et al. Int Immunol 22(1):35-44, 2010
23. Wilcox RA et al. Blood 114(14):2936-2944, 2009
24. Olkhanud PB et al. Cancer Res 71:3505-3515, 2011
25. Wejksza K et al. J Immunol 190(6):2575-2584, 2013
26. Thirukkumaran CM et al. Clin Cancer Res 18(18):4962-4972, 2012
27. Shetty S et al. Int J Hematol 95:274-281, 2012
28. Walters DK et al. Leukemia 25:1344-1353, 2011
29. Bae J et al. Clin Cancer Res 18(17):4850-4860, 2012
30. Avet-Loiseau H et al. Blood 117(6):2009-2011, 2011
31. Grossman V et al. Blood Cancer J 2:e85, 2012
32. Bacher U et al. Cancer Genet Cytogenet 203(2)169-175, 2010
33. Amin S et al. J Cell Mol Med 16(9):2074-2084, 2012
34. Willimott S et al. Br J Haematol 138(6):721-732, 2007
35. Morabito F et al. Br J Haematol 146(1):44-53, 2009
36. Wu X et al. Blood 112(12):4675-4682, 2008
37. Mihalcik SA et al. Cell Cycle 9(24):4884-4892, 2010
38. Bodogai M et al. Cancer Res 73(7):2127-2138, 2013
39. Treppendahl MB et al. Blood 119:206-216, 2012
40. Eyholzer M et al. Leuk Res 34(5):672-676, 2010
41. Makishima H et al. Leuk Res 34(4):447-453, 2010
42. Jankowska AM et al. Blood 113(25):6403-6410, 2009
43. Gondek LP et al. PLoS One 2(11):e1225, 2007
44. Sugimoto Y et al. J Hematol Oncol 5:4, 2012
45. Decot V et al. Biomed Mater Eng 18(1 Suppl):S19-26, 2008
46. Hanson V et al. Tissue Antigens 82(4):269-275, 2013
47. Misund K et al. J Biomol Screen 18(6):637-646, 2013
48. Vitale LA et al. Clin Cancer Res 18(14):3812-3821, 2012
49. Zhou Q et al. Clin Exp Immunology 173(1):131-139, 2013
50. Pahwa R et al. J Immunol Methods 363(1):67-79, 2010

159 products available

Product Name Description Catalog # Size Price Quantity
New Product
Fully automated cell separation from up to 16 samples 23000 1 Unit Request pricing
New Product
RoboSep™-S - The Fully Automated Cell Separator 21000 1 Unit Request pricing
RoboSep™ Buffer Buffer containing FBS 20104 250 mL 19.00 USD
RoboSep™ Filter Tips RoboSep™ filter tips 20125 1 Box of 8 racks 53.00 USD
RoboSep™ Tip Head Polishing Compound RoboSep™ tip head polishing compound 20119 7 mL 25.00 USD
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit
Top Seller
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18387RF For labeling 60 mL of whole blood and bone marrow 618.00 USD
RoboSep™ Human CD138 Positive Selection Kit with Filter Tips
Top Seller
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18357RF For labeling up to 2 x 10e9 cells 641.00 USD
EasySep™ Mouse Neutrophil Enrichment Kit Immunomagnetic isolation of untouched mouse neutrophils 19762 For labeling up to 1 x 10e9 cells 545.00 USD
EasySep™ Human CD56 Positive Selection Kit II
New Product
Immunomagnetic positive selection of human CD56+ cells (e.g. NK cells) 17855 For labeling up to 1 x 10e9 cells 610.00 USD
RoboSep Human CD56 Pos Sel Kit II
New Product
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 17855RF For labeling up to 1 x 10e9 cells Request pricing

Educational Resources


 Fully Automated Cell Isolation with RoboSep™-S
 Ready Sep Go – Cell Separation In As Little As 15 Minutes
 Cell Separation Products for HLA Analysis
 RoboSep™: Solutions for HIV Research
 How Ex Vivo Models Drive Progress in HIV Research
 15-Minute Mouse Cell Isolation
 Instrument Service
 RoboSep™-S Quick Start Procedure


 Frequencies of Cell Types in Human Peripheral Blood Wallchart
 Frequencies and Percentages of Mouse Immune Cell Types
 Antigen Processing and Presentation Wallchart
 Regulatory T Cells Wallchart
 The Immune Response to HIV Poster

Tech bulletin

 Fully Automated Separation of Multiple Cell Types from a Single Sample
 Enhance Sensitivity of FISH Analysis with Purified Multiple Myeloma Cells
 Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis

Scientific Poster

 A rapid new procedure for negative enrichment of monocytes from mouse blood and bone marrow
 Single-step isolation of highly purified, untouched human CD4+ memory T cells by column-free immunomagnetic cell separation
 A simple and versatile method for the isolation of human regulatory T cells from peripheral blood samples ranging from 5 to 500 mL
 A Simple One-Step Method for isolating Highly Purified Plasmacytoid Dendritic Cells from Human Peripheral Blood
 A Simple New Method for Negative Enrichment of Monocytes from Mouse Blood and Bone Marrow
 A Rapid Method to Enrich Specific Lymphocyte Populations (T cells, B cells, Total Lymphocytes) from Whole Blood
 A method for rapid isolation of highly purified monocytes using fully automated negative cell selection
 A Rapid Method for the Isolation of Eosinophils without Ficoll or RBC lysis
 A rapid automated method for the sequential isolation of CD19, CD3 and Myeloid cells from one tube of whole blood
 A Rapid And Efficient Method for The Pre-Enrichment of Dendritic Cells From Human Peripheral Blood Samples
 Isolation and enrichment of rare cells with an imunomagnetic cell selection system
 Isolation of highly purified mouse CD4+CD25+FoxP3+ regulatory T cells in less than 45 minutes


 RoboSep™-S Technical Manual
 RoboSep™ Technical Manual