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RoboSep™

Ready Sep Go

with RoboSep™
Fully Automated, Walk-Away Cell Isolation

Streamline your cell isolations using RoboSep™, the fully automated cell isolation platform. The RoboSep™-S and RoboSep™-16 instruments offer true walk-away automation while retaining the speed and simplicity of EasySep™ with as little as 5 minutes of hands-on time.

Automated cell isolation using RoboSep™ instruments is:

FAST AND EASY. Isolated cells are immediately ready for flow cytometry, functional studies or other downstream applications.
VERSATILE. Virtually any cell type can be isolated from a wide range of sample sources and sizes (e.g. whole blood, Leuko Paks, PBMCs, splenocytes) using positive or negative selection protocols.
EFFICIENT. Perform sequential or simultaneous cell isolations from up to 4 samples with RoboSep™-S and up to 16 samples with RoboSep™-16. See Florida Hospital Case Study.
SAFE. With a column-free design and minimal sample handling required, the risk of exposure to dangerous pathogens is minimized.
FREE FROM CROSS-CONTAMINATION. Disposable tips and a column-free system eliminate the risk of sample cross-contamination.
HIGHLY CUSTOMIZABLE. For special or non-standard applications, our experts can work with you to design customized cell separation protocols to isolate cells of interest.
EASY TO MAINTAIN. No daily washing or decontamination is required.

Comprehensive instrument service packages minimize unplanned downtime that can negatively impact lab efficiency. Our Technical Support experts can quickly access diagnostic logs to assist with troubleshooting.

To view cell isolation kits for use with RoboSep™ instruments, click here.
RoboSep

Explore Tools for Automated Cell Isolation
RoboSep™-S       |       RoboSep™-16       |       Featured Applications       |       Educational Resources

RoboSep™-S Tour


By combining an exceptionally compact design, a simple, user-friendly interface and the fast and easy cell separation protocols you’ve come to expect from STEMCELL Technologies, RoboSep™-S fits easily into the workflow of any lab that needs the multi-sample processing capacity, speed, reliability and convenience of automated cell isolation.

    


HIGHLIGHTED FEATURES

Compact Design Reagent and Experiment Tracking


Exceptionally small footprint (42 cm x 42 cm) frees up valuable lab space
Place multiple units side-by-side in biological safety cabinets or on lab benches



Integrated barcode reader and End-of-Run reports track user, reagent and protocol details

Simultaneous or Sequential Cell Isolation Quick Start Options


Simultaneous cell isolations for up to 4 samples or sequential isolation of up to 4 cell types from the same sample



Start a cell separation protocol in just 5 minutes with minimal touchscreen navigation
Use pre-programmed user profiles or custom profiles with commonly run protocols



RoboSep™-16 Tour


By combining enhanced liquid-handling capabilities, a simple, user-friendly interface and the fast and easy cell separation protocols RoboSep™-16 allows high volume users that need the multi-sample processing capacity, speed, reliability and convenience of automated cell isolation.

    


HIGHLIGHTED FEATURES

Simultaneous or Sequential Cell Isolation Reagent Detection
RoboSep™-16

RoboSep™-16 enables simultaneous cell isolation from up to 16 samples or sequential isolation of 4 different cell types from 4 different samples

RoboSep™-16

Conductive pipette tips automatically detect EasySep™ reagent levels and utilize spare reagents when required

Quick Start Options Minimal Sample Handling with no Cross-Contamination
RoboSep™-16

Start a cell separation protocol in as little as 5 minutes with an easy user interface
Use pre-programmed user profiles or custom profiles with commonly run protocols

RoboSep™-16

Automated sample handling with disposable pipette tips reduces the risk of exposure to dangerous pathogens and eliminates the risk of sample cross-contamination


Featured Applications

Immunology and Infectious Disease Research
RoboSep™ minimizes the risk associated with handling hazardous biological specimens and has been used to support studies on:
Pathogens including HIV, other viruses and bacteria1-9
(Case Study: HIV Research Profiles - How Ex Vivo Models Drive Progress in HIV Research)
Autoimmune diseases including diabetes and rheumatoid arthritis10-15
Transplantation immunology16-22
Cancer23-25

Hematological Malignancies
RoboSep™ effectively enriches rare cell types to enhance assay sensitivity for research on:
Multiple Myeloma26-32
Chronic Lymphocytic Leukemia33-38
Acute Myeloid Leukemia39-40
Myelodysplastic Syndrome41-44

Chimerism and HLA Testing
RoboSep™ facilitates simultaneous or sequential cell isolation with reliable and reproducible results for:
Chimerism analysis45,46
(Case Study: Fully Automated Sequential Cell Isolation of Four Different Cell Types from a Single Sample with RoboSep™)
Flow Cytometry Crossmatch (FCXM)
Serology-based assays

Assay Development and Immunotherapy Research
RoboSep™ has been used to isolate highly purified, functional cells from a variety of sample sources for:
Drug discovery and development15,47-49
Immune cell-based assay development
Vaccine development29
Basic cellular therapy research50


Educational Resources

Video
RoboSep™-S Introduction
Webinar
Fully Automated Cell Separation for Chimerism Analysis with RoboSep™

HIV Research Profiles
How Ex Vivo Models Drive Progress in HIV Research
Technical Bulletin
Enhance Sensitivity of FISH Analysis with Purified Multiple Myeloma Cells

Technical Bulletin
Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
Wallchart
Frequencies of Cell Types in Normal Human Peripheral Blood




References
1. Miles B et al. J Leuk Biol 94(2):281-289, 2013
2. Prince LR et al. PLoS One 7(2):e31506, 2012
3. Cohen S et al. JAMA 309(7):699-705, 2013
4. Wilen CB et al. PLoS Pathog 7(4):e1002020, 2011
5. Sáez-Cirión A et al. Nat Protoc 5:1033-1041, 2010
6. Sáez-Cirión A et al. Blood 118(4):955-964, 2011
7. Ndhlovu ZM et al. J Virol 86(12):6959-6969, 2012
8. Vetter M et al. J Virol 83(17):8646-8654, 2009
9. Chun T-W et al. AIDS 24(18):2803-2808, 2010
10. Martinez-Llordella M et al. J Exp Med 210(8):1603-1619, 2013
11. Montandon R et al. PNAS 110(24):E2199-2208, 2013
12. Esensten JH et al. J Immunol 183(1):75-82, 2009
13. O’Boyle G et al. PNAS 109(12):4598-4603, 2012
14. Karnell JL et al. J Immunol 187(7):3603-3612, 2011
15. Durand CA et al. Autoimmunity 46(1):62-73, 2013
16. Haniffa M et al. J Exp Med 206(2):371-385, 2009
17. Costa C et al. Trans Proceed 43(4):1159-1161, 2011
18. Mavin E et al. Transplantation 94(5):456-464, 2012
19. Kirkiles-Smith NC et al. Transplantation 87(2):189-197, 2009
20. Søndergaard HS et al. Clin Exp Immunol 172(2):300-310, 2013
21. Wang X-N et al. Transplantation 88(2):188-197, 2009
22. Hüser N et al. Int Immunol 22(1):35-44, 2010
23. Wilcox RA et al. Blood 114(14):2936-2944, 2009
24. Olkhanud PB et al. Cancer Res 71:3505-3515, 2011
25. Wejksza K et al. J Immunol 190(6):2575-2584, 2013
26. Thirukkumaran CM et al. Clin Cancer Res 18(18):4962-4972, 2012
27. Shetty S et al. Int J Hematol 95:274-281, 2012
28. Walters DK et al. Leukemia 25:1344-1353, 2011
29. Bae J et al. Clin Cancer Res 18(17):4850-4860, 2012
30. Avet-Loiseau H et al. Blood 117(6):2009-2011, 2011
31. Grossman V et al. Blood Cancer J 2:e85, 2012
32. Bacher U et al. Cancer Genet Cytogenet 203(2)169-175, 2010
33. Amin S et al. J Cell Mol Med 16(9):2074-2084, 2012
34. Willimott S et al. Br J Haematol 138(6):721-732, 2007
35. Morabito F et al. Br J Haematol 146(1):44-53, 2009
36. Wu X et al. Blood 112(12):4675-4682, 2008
37. Mihalcik SA et al. Cell Cycle 9(24):4884-4892, 2010
38. Bodogai M et al. Cancer Res 73(7):2127-2138, 2013
39. Treppendahl MB et al. Blood 119:206-216, 2012
40. Eyholzer M et al. Leuk Res 34(5):672-676, 2010
41. Makishima H et al. Leuk Res 34(4):447-453, 2010
42. Jankowska AM et al. Blood 113(25):6403-6410, 2009
43. Gondek LP et al. PLoS One 2(11):e1225, 2007
44. Sugimoto Y et al. J Hematol Oncol 5:4, 2012
45. Decot V et al. Biomed Mater Eng 18(1 Suppl):S19-26, 2008
46. Hanson V et al. Tissue Antigens 82(4):269-275, 2013
47. Misund K et al. J Biomol Screen 18(6):637-646, 2013
48. Vitale LA et al. Clin Cancer Res 18(14):3812-3821, 2012
49. Zhou Q et al. Clin Exp Immunology 173(1):131-139, 2013
50. Pahwa R et al. J Immunol Methods 363(1):67-79, 2010

Featured Products

Catalog # 21000
RoboSep™-S - The Fully Automated Cell Separator
Catalog # 23000
RoboSep™-16 - Fully Automated Cell Separator for up to 16 Samples
Catalog # 18387
Immunomagnetic Positive Selection Kit
Catalog # 20104
Cell Separation Buffer
Catalog # 20125
RoboSep™ Accessories

171 products available

Narrow Results By:
Product Name Description Catalog # Size Price Quantity
RoboSep™-16
New Product
Fully automated cell separation from up to 16 samples 23000 1 Unit Request pricing
RoboSep™-S
New Product
RoboSep™-S - The Fully Automated Cell Separator 21000 1 Unit Request pricing
RoboSep™ Buffer Buffer containing FBS 20104 250 mL 19.00 USD
RoboSep™ Filter Tips RoboSep™ filter tips 20125 1 Box of 8 racks 53.00 USD
RoboSep™ Tip Head Polishing Compound RoboSep™ tip head polishing compound 20119 7 mL 25.00 USD
RoboSep™ Human Whole Blood and Bone Marrow CD138 Positive Selection Kit
Top Seller
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18387RF For labeling 60 mL of whole blood and bone marrow 631.00 USD
RoboSep™ Human CD138 Positive Selection Kit with Filter Tips
Top Seller
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18357RF For labeling up to 2 x 10e9 cells 656.00 USD
EasySep™ Mouse Neutrophil Enrichment Kit Immunomagnetic isolation of untouched mouse neutrophils 19762 For labeling up to 1 x 10e9 cells 555.00 USD
EasySep™ Mouse CD19 Positive Selection Kit II
New Product
Immunomagnetic positive selection of CD19+ cells (e.g. B cells) from mouse splenocytes or other tissues 18954 For labeling up to 2 x 10e9 cells 620.00 USD
RoboSep™ Mouse CD19 Positive Selection Kit II
New Product
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips 18954RF For labeling up to 2 x 10e9 cells 684.00 USD

Featured Resources

Brochure

  • Fully Automated Cell Isolation with RoboSep™-S
  • Tools For Your Immunology Research
  • Cell Separation Products for HLA Analysis
  • RoboSep™: Solutions for HIV Research
  • How Ex Vivo Models Drive Progress in HIV Research
  • 15-Minute Mouse Cell Isolation
  • Instrument Service
  • RoboSep™-S Quick Start Procedure
  • Wallchart

  • Frequencies of Cell Types in Human Peripheral Blood Wallchart
  • Frequencies and Percentages of Mouse Immune Cell Types
  • Antigen Processing and Presentation Wallchart
  • Regulatory T Cells Wallchart
  • The Immune Response to HIV Poster
  • Tech bulletin

  • Fully Automated Separation of Multiple Cell Types from a Single Sample
  • Enhance Sensitivity of FISH Analysis with Purified Multiple Myeloma Cells
  • Purity Assessment by Flow Cytometry for Lineage-Specific Chimerism Analysis
  • Manual

  • RoboSep™-S Technical Manual
  • RoboSep™ Technical Manual
  • Scientific Poster

  • A rapid new procedure for negative enrichment of monocytes from mouse blood and bone marrow
  • Single-step isolation of highly purified, untouched human CD4+ memory T cells by column-free immunomagnetic cell separation
  • A simple and versatile method for the isolation of human regulatory T cells from peripheral blood samples ranging from 5 to 500 mL
  • A Simple One-Step Method for isolating Highly Purified Plasmacytoid Dendritic Cells from Human Peripheral Blood
  • A Simple New Method for Negative Enrichment of Monocytes from Mouse Blood and Bone Marrow
  • A Rapid Method to Enrich Specific Lymphocyte Populations (T cells, B cells, Total Lymphocytes) from Whole Blood
  • A method for rapid isolation of highly purified monocytes using fully automated negative cell selection
  • A Rapid Method for the Isolation of Eosinophils without Ficoll or RBC lysis
  • A rapid automated method for the sequential isolation of CD19, CD3 and Myeloid cells from one tube of whole blood
  • A Rapid And Efficient Method for The Pre-Enrichment of Dendritic Cells From Human Peripheral Blood Samples
  • Isolation and enrichment of rare cells with an imunomagnetic cell selection system
  • Isolation of highly purified mouse CD4+CD25+FoxP3+ regulatory T cells in less than 45 minutes