EpiCult® for Mammary Cells

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EpiCult™ supports mammary epithelial monolayer culture with defined culture media. EpiCult™-C is optimized for the routine culture of primary human mammary epithelial cells, while EpiCult™-B has species-specific formulations for the culture and assay of either human or mouse mammary cells. EpiCult™-B (Human) can assay for the presence of human mammary precursor cells in either mammary colony-forming cell (Ma-CFC) assay or in 3D Matrigel™-based cultures. Quantifying Ma-CFC frequency provides a functional measure of the number of mammary progenitor cells in the sample, while the 3D morphogenesis assay enables more representative studies of the in vivo mammary duct structure. EpiCult™-B (Mouse) can similarly assay for the presence of mouse mammary colony-forming cells in either adherent monolayer or in 3D Matrigel™-based cultures. As this medium is formulated specifically for mouse cells, it delays the epithelial-to-mesenchymal transition (EMT) commonly observed in the mouse mammary epithelial in vitro cultures.

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Product Name	Description	Catalog #	Size	Price
0.2% Heparin Sodium Salt in PBS	0.2% heparin sodium salt in phosphate buffered saline	07980	2 mL	30.00 USD
40 µm Cell Strainer	Sterile, 40 µm cell strainer	27305	50/Case	143.00 USD
bFGF, Human, Recombinant	Basic fibroblast growth factor	02634	25 µg	349.00 USD
Carmine Alum	Dye used for staining of mammary gland whole mounts from mice	07070	100 mL	27.00 USD
Collagenase/Hyaluronidase	10X collagenase/hyaluronidase in DMEM	07912	10 mL	158.00 USD
Dispase (5 mg/mL)	5 mg/mL dispase in Hank's balanced salt solution modified	07913	100 mL	158.00 USD
DNase I Solution (1 mg/mL)	Deoxyribonuclease I solution (1 mg/mL)	07900	1 mL	42.00 USD
EpCAM Antibody, Clone 323/A3	Mouse monoclonal antibody to human EpCAM	01421	100 µg	165.00 USD
EpCAM Antibody, Clone VU-1D9	Mouse monoclonal antibody to human EpCAM	01420	100 µg	165.00 USD
EpCAM Antibody, Clone VU-1D9, FITC-Conjugated	Mouse monoclonal antibody to human EpCAM - FITC conjugated	10109	100 Tests	171.00 USD