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Recommended for:
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• Cord blood facilities interested in standardized colony identification and enumeration to assess cord blood progenitor cell content
• Increasing proficiency in assay set-up and colony identification
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Components:
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Theory and Applications:
• Overview of hematopoiesis and introduction to colony identification
• Technical tips and standardization of media products for the CFC assay using cord blood
• Procedures for processing cord blood for CFC analysis
• Detection and quantitation of progenitors in cord blood for manual and automated systems
• Differences in colony identification for unprocessed fresh versus processed and frozen samples
Practical Laboratory Sessions:
• Standard procedures for setting up the CFC assays using MethoCult™
• Identification of hematopoietic colonies derived from CB CFCs
• Processing cord blood samples for the CFC assay
• Comparative counting exercise with instructors and course participants
• Overview of maintenance of incubators for optimal cultures
• Evaluation of CD34+ population using flow cytometry
• Introduction to STEMvision™ for automated and standardized CFC (7-day) assay
Registration Includes:
• Dinner at an award-winning west coast restaurant
• Refreshments
• Lunch
• Instructional materials
• Certificate issued upon successful completion of the training course
Where:
STEMCELL Technologies
Suite 400 570 West 7th Avenue
Vancouver, BC, Canada
V5Z 1B3
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Intended Use Statement:
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For Research Use Only. Not for Therapeutic or Diagnostic Use.
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