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Take the "if" out of "diff"
Reduce Variability by Differentiating with STEMdiff™
When working with human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells) , even the most detailed and rigorously-followed protocols can still lead to inconsistent differentiation.1,2 Use STEMdiff™ products for reproducible differentiation across multiple ES cell and iPS cell lines.
STEMdiff™ is part of the world’s most complete system of defined reagents for human ES cells and iPS cells – a system that includes mTeSR™1, the most published feeder-free ES cell and iPS cell culture medium, and AggreWell™, for the formation of uniform embryoid bodies. To minimize reagent variability, the formulations, raw material specifications and manufacturing processes for all STEMdiff™ products have been carefully optimized. In addition, STEMdiff™ products include detailed, user-friendly protocols to help standardize your differentiation procedures.
Definitive endoderm
progenitors derived using
STEMdiff™ Definitive
Endoderm Kit: SOX17
(red), FOXA2 (green). |
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STEMdiff™ Definitive Endoderm Kit: For Animal Component-Free, Efficient Differentiation
The STEMdiff™ Definitive Endoderm Kit enables differentiation of human embryonic stem cells and induced pluripotent stem cells to multipotent definitive endoderm.
Advantages of STEMdiff™ Definitive Endoderm Kit:
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Fully defined, serum-free and animal component-free |
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Optimized for use with cells maintained in mTeSR™1 or TeSR™2 cell culture media |
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Efficient and reproducible differentiation of multiple ES cell and iPS cell lines |
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Differentiated cells co-express SOX17, CXCR4, FOXA2 and c-KIT
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Convenient, user-friendly format and protocol |
Definitive endoderm generated using this kit can be further differentiated to multiple downstream endodermal cell lineages including hepatic, pancreatic, intestinal and pulmonary.
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Neural rosettes derived
using STEMdiff™ Neural Induction Medium: SOX1 (green), ZO-1 (red), DAPI (blue).
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STEMdiff™ Neural Induction Medium: For Efficient and Serum-Free Differentiation
STEMdiff™ Neural Induction Medium enables differentiation of human embryonic stem cells and induced pluripotent stem cells into neural progenitor cells (NPCs).3,4
Advantages of STEMdiff™ Neural Induction Medium:
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Fully defined and serum-free medium
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Optimized for use with cells maintained in mTeSR™1 or TeSR™2
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Efficient and reproducible differentiation of multiple ES cell and iPS cell lines
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Rapid neural induction - neural rosettes within six days
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Convenient, user-friendly format and protocol
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Neural rosette clusters, a sign of early neural differentiation, can be selected and isolated for further experiments using STEMdiff™ Neural Rosette Selection Reagent. The resulting NPCs can then be expanded and cryopreserved using STEMdiff™ Neural Progenitor Medium and STEMdiff™ Neural Progenitor Freezing Medium (both coming soon).
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Cardiomyocytes (left), hematopoietic cells (right) and definitive endoderm (bottom), derived using STEMdiff™ APEL™ Medium. |
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STEMdiff™ APEL™ Medium: For Versatile, Growth Factor-Directed Differentiation
STEMdiff™ APEL™ Medium is an animal component-free cell culture medium for differentiation of human embryonic stem cells and induced pluripotent stem cells to a variety of lineages when lineage-specific growth factors are added.
Advantages of STEMdiff™ APEL Kit:
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Defined and animal component-free
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Optimized for use with cells maintained in mTeSR™1 medium
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Versatile, growth factor-free formulation |
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Published protocols for differentiation to ectoderm5, endoderm5 and mesoderm6,7
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As a neutral yet supportive cell culture medium, STEMdiff™ APEL™ allows researchers to customize their differentiation protocols, and discover new regulatory factors or pathways. Compatible with both adherent culture or embryoid body (EB)-based protocols.
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Uniform embryoid bodies formed using AggreWell™ plates.
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AggreWell™: For Uniform Embryoid Body Formation
Many protocols for differentiating human embryonic stem cells and induced pluripotent stem cells start with the formation of embryoid bodies (EBs). Conventional EB formation methods result in EBs of heterogeneous size and shape, leading to inefficient and uncontrolled differentiation.8,9 AggreWell™ plates allow users to produce uniformly-shaped EBs of controlled size, making differentiation experiments more reproducible.10
Advantages of AggreWell™ Plates:
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Forms EBs of uniform size and shape
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Enables the user to control size from 10 cells to 20,000 cells per EB
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Reduces variability in differentiation protocols that utilize EBs
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Suitable for high-throughput procedures
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STEMdiff™ Cardiomyocyte Kit: IN DEVELOPMENT
The STEMdiff™ Cardiomyocyte Kit is designed for efficient differentiation of human induced pluripotent stem cells into beating cardiomyocytes.
Advantages of STEMdiff™ Cardiomyocyte Kit:
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Fully defined and serum-free
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Optimized for use with cells maintained in mTeSR™1
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Efficient and reproducible differentiation of multiple iPS cell lines
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Generates spontaneously contracting cardiomyocytes
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References
- D’Amour KA et al. Efficient differentiation of human embryonic stem cells to definitive endoderm. Nature Biotechnology 23(12):1534-41, 2005
- Kattman SJ et al. Stage-Specific Optimization of Activin/Nodal and BMP Signaling Promotes Cardiac Differentiation of Mouse and Human Pluripotent Stem Cell Lines. Cell Stem Cell 8(2): 228-240, 2011
- Linta L et al. Calcium activated potassium channel expression during human iPS cell-derived neurogenesis. Ann Anat, 2013 (epub ahead of print)
- Xia G et al. Generation of Human-Induced Pluripotent Stem Cells to Model Spinocerebellar Ataxia Type 2 In Vitro. J Mol Neurosci, 2012 (epub ahead of print)
- Ng ES et al. A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies. Nature Protocols 3(5): 768-776, 2008
- Tan JY et al. Efficient Derivation of Lateral Plate and Paraxial Mesoderm Subtypes from Human Embryonic Stem Cells Through GSKi-Mediated Differentiation. Stem Cells Dev, 2013 (epub ahead of print)
- Protocol adapted from Chadwick et al. 2003 and Ng et al. 2008, and discussed in this Technical Bulletin
- Bauwens CL et al. Control of human embryonic stem cell colony and aggregate size heterogeneity in?uences differentiation trajectories. Stem Cells 26(9): 2300-2310, 2008
- Bratt-Leal AM, et al. Engineering the embryoid body microenvironment to direct embryonic stem cell differentiation. Biotechnol Prog 25(1): 43-51, 2009
- Yanai A, et al. Differentiation of Human Embryonic Stem Cells Using Size-Controlled Embryoid Bodies and Negative Cell Selection in the Production of Photoreceptor Precursor Cells. Tissue Eng Part C Methods, 2013 (epub ahead of print)
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