Tissue Dissociation and Digestion Protocols
Technical tip from our dedicated team of Product and Scientific Support specialists
Find protocols and reagents for preparing single-cell suspensions or tissue fragments for use in downstream procedures such as cell separation, cell culture, or organoid culture.
Tissue Dissociation for Single-Cell Suspension
How to Prepare a Single-Cell Suspension from Mouse Spleen
Dissociation of Mouse Solid Tumors into Single-Cell Suspension
Dissociation of Mouse Lung Tissue into Single-Cell Suspension
Dissociation of Adult Mouse or Rat CNS Tissue into Single-Cell Suspension
More protocols for generating single-cell suspensions can be found in the following documents:
(enzymatic dissociation protocol)
Note: Enzymatic spleen digestion is only recommended for the isolation of CD11c+ cells using EasySep™ (e.g. EasySep™ Mouse Pan-DC Enrichment Kit II or EasySep™ Mouse CD11c Positive Selection Kit II).
Visit our Methods Library to explore more protocols and technical tips for preparing single-cell suspensions.
Tissue Dissociation for Tissue Fragments or Pieces
How to Isolate Mouse Intestinal Crypts
How to Isolate Human Colonic Crypts from Intestinal Biopsies
More protocols can be found in the following documents:
Tissue and Cell Culture Dissociation Reagents
Find reagents for tissue and cell dissociation, such as the following tissue digestion enzymes:
- Collagenase/Hyaluronidase (e.g. 10X Collagenase/hyaluronidase in DMEM)
- Dispase (e.g. 1 U/mL dispase in DMEM/F-12)
- Collagenase (e.g. Collagenase Type I)
- NeuroCult™ Enzymatic Dissociation Kit for Adult CNS Tissue (Mouse and Rat)
- Spleen Dissociation Medium
Unsure which dissociation reagent to choose? We can help. Contact us by phone or email or use the LiveChat function on this page to discuss your specific needs and applications.
Downstream Applications
Cell Isolation Technologies
Explore technologies for efficient isolation of cell subsets from your single-cell suspension.
Cell Culture Media and Supplements
Find reagents for culturing cells or establishing organoids from tissue fragments.
Antibodies
Choose from our large selection of antibodies to analyze your single-cell suspension.
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