Figure 1. Morphology of hPSCs Maintained in TeSR™2 is Comparable to hPSCs Cultured in mTeSR™1

(A,B) Undifferentiated human ES (H9) cells cultured on Corning® Matrigel® matrix in TeSR™2 retain the prominent nucleoli and high nuclear-to-cytoplasm ratio characteristic of this cell type. Densely packed cells and multi-layering are apparent when cells are ready to be passaged. (C,D) H9 cells cultured under the same conditions in mTeSR™1 exhibit comparable morphology.

Figure 2. Fold and Cumulative Aggregate Expansion in TeSR™2

Graph shows the average fold expansion per passage ± SEM obtained for human ES and iPS cells cultured in mTeSR™1 (brown) or TeSR™2 (red) with Corning® Matrigel® over 10 passages. Expansion was determined by counting the cell aggregates obtained at harvest and dividing by the number of cell aggregates seeded.
Note: This data is representative of cultures passaged after 5-6 days in culture, lower expansion should be expected if using shorter culture times.

Figure 3. hESCs Cultured in TeSR™2 Are Pluripotent

H9 cells were cultured for 11 passages in TeSR™2, then injected subcutaneously into NOD-SCID mice. The resulting teratomas contained cell types from all 3 germ layers. Representative tissue types are shown.

Figure 4. Human Pluripotent Stem Cells Cultured in TeSR™2 Retain Expression of Undifferentiated Cell Markers

Histogram analysis for H9 human ES and WLS-1C human iPS cells characterized using flow cytometry for undifferentiated cell markers (SSEA-3 and OCT3/4) after passaging in TeSR™2 for 21 passages (WLS-1C) and 18 passages (H9) respectively (filled histogram = sample, hollow histogram = secondary antibody only).

Figure 5. Human ES Cells Cultured Long-Term in TeSR™2 Retain Normal Karyotype

Chromosomal analysis of H9 hES cells cultured in TeSR™2 for 12 passages shows that normal karyotype is retained during passaging.