TeSR™-AOF 3D

Animal origin-free suspension culture medium for human ES and iPS cells

TeSR™-AOF 3D

Animal origin-free suspension culture medium for human ES and iPS cells

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Animal origin-free suspension culture medium for human ES and iPS cells
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Product Advantages

⦁ Minimize risk of your choice of ancillary materials by selecting a medium with no animal raw materials to the secondary level of manufacturing

⦁ Reduce time and labor with a fed-batch feeding strategy that does not require full-medium changes

⦁ Scale up high-quality hPSCs rapidly without requiring adaptation from 2D culture

What's Included

  • TeSR™-AOF 3D Seed Basal Medium, 480 mL
  • TeSR™-AOF 3D Seed Supplement, 20 mL
  • TeSR™-AOF 3D Feed Supplement, 20 mL

Overview

TeSR™-AOF 3D is an animal origin-free culture medium developed for expansion and scale-up of undifferentiated human embryonic stem (ES) cells and human induced pluripotent stem (iPS) cells as aggregates in 3D suspension culture. Based on TeSR™-AOF, TeSR™-AOF 3D is a robust medium with an optimized fed-batch protocol for scaling up human ES and iPS cell production in suspension culture. The novel fed-batch workflow saves time and media; daily feeds replenish nutrients and eliminate the need for medium exchanges on non-passaging days.

The TeSR™-AOF 3D system is compatible with cells previously cultured in mTeSR™1 (Catalog #85850), mTeSR™ Plus (Catalog #100-0276), TeSR™-AOF (Catalog #100-0401), or TeSR™-E8™ (Catalog #05990) on Corning® Matrigel® hESC-Qualified Matrix (Corning Catalog #354277), Vitronectin XF™ (Catalog #07180), or CellAdhere™ Laminin-521 (Catalog #77003). TeSR™-AOF 3D is compatible with a range of suspension culture vessels.

No materials of animal or human origin are used in the manufacture of this medium or its components, to at least the secondary level of manufacturing. TeSR™-AOF 3D is animal component-free to the secondary level. Each lot of TeSR™-AOF 3D Seed or Feed Supplement that is used to prepare complete TeSR™-AOF medium is performance-tested in a culture assay using human pluripotent stem cells.
Subtype
Specialized Media
Cell Type
Pluripotent Stem Cells
Application
Cell Culture
Brand
TeSR
Area of Interest
Disease Modeling, Stem Cell Biology, Cell Therapy Development
Formulation Category
Animal Origin-Free

Data Figures

Growth of Human Pluripotent Stem Cells (hPSCs) in TeSR™-AOF 3D

Figure 1. Growth of Human Pluripotent Stem Cells (hPSCs) in TeSR™-AOF 3D

TeSR™-AOF 3D supports the expansion and high viability of hPSCs over multiple passages in aggregate suspension culture. Shown are (A) cumulative viable cells, (B) daily fold expansion, and (C) end-of-passage viabilities in human ES cell lines (H1 and H7) and human iPS cell lines (WLS-1C and STiPS-F016) over 5 passages. Error bars represent ± SD, n = 3.

hPSCs Cultured in TeSR™-AOF 3D Express Markers of the Undifferentiated State and Differentiate to the Three Germ Layers.

Figure 2. hPSCs Cultured in TeSR™-AOF 3D Express Markers of the Undifferentiated State and Differentiate to the Three Germ Layers

Shown are (A) OCT-4 and TRA-1-60 expression and (B) trilineage differentiation potential (ectoderm: % Nestin+/Pax6+, mesoderm: % Brach+, and endoderm: % Sox-17+/CXCR4+) using the STEMdiff™ Trilineage Differentiation Kit for multiple hPSC lines after 5 passages in TeSR™-AOF 3D. Error bars represent ± SD, n = 3.

Metabolic Profile of hPSCs During Culture in TeSR™-AOF 3D

Figure 3. Metabolic Profile of hPSCs During Culture in TeSR™-AOF 3D

(A) Glucose concentration in TeSR™-AOF 3D is maintained at optimal levels throughout the culture period. Dotted line represents the glucose level in TeSR™-AOF 3D Complete Seed Medium. (B) Lactate concentration is maintained at low levels (below 15 mM as indicated by the dotted line) throughout the culture period for multiple hPSC lines after 5 passages in TeSR™-AOF 3D. Error bars represent ± SD, n = 3.

Morphology of hPSC Aggregates in TeSR™-AOF 3D

Figure 4. Morphology of hPSC Aggregates in TeSR™-AOF 3D

Multiple hPSC lines maintained in TeSR™-AOF 3D depict morphology characteristic of hPSC aggregates in suspension culture, namely a mostly spherical shape, clear edges (not smooth or shiny), even color, a dimpled or pockmarked appearance, and are less than 400 µm in size.

Protocols and Documentation

Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.

Document Type
Product Name
Catalog #
Lot #
Language
Product Name
TeSR™-AOF 3D
Catalog #
100-0720
Lot #
All
Language
English
Document Type
Technical Manual
Product Name
TeSR™-AOF 3D
Catalog #
100-0720
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
TeSR™-AOF 3D
Catalog #
100-0720
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
TeSR™-AOF 3D
Catalog #
100-0720
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
TeSR™-AOF 3D
Catalog #
100-0720
Lot #
All
Language
English

Applications

This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.