Genome Editing of Intestinal Organoids

Organoids are helping scientists push the boundaries for in vitro study of the intestinal epithelium. A key benefit of intestinal organoid cultures is that they are amenable to genome editing, such as by CRISPR-Cas9, an RNA-guided technology that has revolutionized genome editing due to its ease and efficiency. Explore our protocols and resources for information on how to perform high-efficiency CRISPR-Cas9 genome editing of intestinal organoids to develop better in vitro disease models and study organ development.