Expansion of CD34+ Cells in Serum-Free Culture
Technical tip from our dedicated team of Product and Scientific Support specialists
StemSpan™ serum-free expansion media is recommended for the expansion and culture of hematopoietic cells, when supplemented with appropriate cytokines and supplements. We offer a number of StemSpan™ Cytokine Cocktails (CC) and Supplements optimized for the expansion of human CD34+ progenitors cells in serum-free culture. To help you select the appropriate StemSpan™ cytokine supplement for your application, we’ve outlined some of the features and differences between these products below:
*NOTE: The magnitude of expansion of hematopoietic cells and progenitors is variable between donors and is also dependent on a number of factors including: the cell source, input cell population, culture medium, cell density, time in culture and refeeding or passaging procedures.
These StemSpan™ Cytokine Cocktails and Supplements are intended for use in combination with our StemSpan™ serum-free expansion media, including StemSpan™ SFEM, SFEM II and ACF.
For further information on our StemSpan™ products, please contact techsupport@stemcell.com.
PRODUCT |
CATALOG # (SIZE) |
COMPONENTS |
FEATURES |
PERFORMANCE* (in 7 day cultures) |
StemSpan™ CC100 |
02690 (1 mL, 100X) |
|
|
~20-60-fold at most ~10-fold |
StemSpan™ CC110 |
02697 (1 mL, 100X) |
|
|
|
StemSpan™ CD34+ Expansion Supplement |
02691 (10 mL, 10X) |
|
|
~20-60-fold >10 to 40-fold |
These StemSpan™ Cytokine Cocktails and Supplements are intended for use in combination with our StemSpan™ serum-free expansion media, including StemSpan™ SFEM, SFEM II and ACF.
For further information on our StemSpan™ products, please contact techsupport@stemcell.com.
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