Impact of Feeding Strategies on Expansion of Human Pluripotent Stem Cells in Stirred Tank Bioreactors Using mTeSR™1

Suspension culture of human iPS and ES cells, using batch feeding or perfusion feeding regimes

The routine application of human pluripotent stem cells (hPSCs) and their derivatives in regenerative medicine and drug discovery will require the constant supply of high cell numbers generated by robust and well-defined processes. Previous studies showed that mTeSR™1 is the most supportive medium for a novel hPSC culture strategy, namely hPSC propagation and expansion of 3-dimensional (3D) aggregates in suspension culture. This technology has the potential to overcome numerous limitations of conventional surface-adherent, 2-dimensional (2D) cultivation of hPSCs.

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