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How to Prepare a Single-Cell Suspension from Mouse Spleen
This video demonstrates how to harvest cells from a spleen sample, and prepare a single cell suspension prior to performing cell isolation. Preparing a true single cell suspension of the primary tissue sample will optimize cell separation from tissues by avoiding additional cell loss and enabling maximum labeling of the target cells.
Publish Date: October 11, 2012
Views: 54238
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36:48Applications of the Hematopoietic CFU Assay in Toxicity Testing
Publish Date: January 17, 2020 Views: 5 -
16:20Immunometabolism: Metabolic Control of T Cell Differentiation and Function
Publish Date: November 21, 2019 Views: 187 -
20:15STEMCELL Seminar: At the Origin of Intestinal Epithelial Stem Cells and Beyond
Publish Date: November 20, 2019 Views: 12 -
21:26STEMCELL Seminar: Functional Applications of Intestinal Organoids
Publish Date: November 20, 2019 Views: 29 -
17:44STEMCELL Seminar: Advances in Brain Organoid Technologies and their Applications to Human Biology
Publish Date: November 20, 2019 Views: 30 -
20:09Nature Research Round Table: Maintenance of Human Pluripotent Stem Cells In Vitro
Publish Date: November 01, 2019 Views: 41 -
21:35Nature Research Round Table: Regulations Around Human Induced Pluripotent Stem Cell Registration
Publish Date: November 01, 2019 Views: 14 -
13:01Nature Research Round Table: HLA Typing Considerations for Human Pluripotent Stem Cell Banking
Publish Date: November 01, 2019 Views: 17 -
13:02Nature Research Round Table: Standards for Pluripotent Stem Cell Banking
Publish Date: November 01, 2019 Views: 31 -
33:47Nature Research Round Table: Defining and Maintaining Pluripotency & hPSC Line Registration and Banking - Panel Discussion
Publish Date: November 01, 2019 Views: 115 -
16:56Nature Research Round Table: Identifying Acquired and Background Genetic Variants in Human Pluripotent Stem Cells
Publish Date: November 01, 2019 Views: 18 -
17:45Nature Research Round Table: Genome Editing in Human Pluripotent Stem Cells
Publish Date: November 01, 2019 Views: 18 -
18:48Nature Research Round Table: The Process of Human Pluripotent Stem Cell Adaptation
Publish Date: November 01, 2019 Views: 14 -
15:47Nature Research Round Table: Genomic Integrity of Human Pluripotent Stem Cells
Publish Date: November 01, 2019 Views: 12 -
31:25Nature Research Round Table: Best Practices for the QC of Genome-Edited hPSC Lines - Panel Discussion
Publish Date: November 01, 2019 Views: 24 -
19:35Nature Research Round Table: Retinal Cell Therapy Using Human Embryonic Stem Cells
Publish Date: November 01, 2019 Views: 8 -
24:46Nature Research Round Table: Parkinson's Disease Therapy with Human Embryonic Stem Cells
Publish Date: November 01, 2019 Views: 24 -
17:30Nature Research Round Table: Human Pluripotent Stem Cell Lines as Disease Models
Publish Date: November 01, 2019 Views: 9 -
29:03Nature Research Round Table: hPSC Lines for Cell Therapies - Panel Discussion
Publish Date: November 01, 2019 Views: 21 -
3:39Counting hPSC Aggregates for Maintenance Culture and Differentiation
Publish Date: October 14, 2019 Views: 318 -
17:18The Impact of Ancillary Materials on Your Translational Research
Publish Date: September 11, 2019 Views: 91 -
1:07:14Optimized Workflows for High-Efficiency Genome Editing in Stem and Primary Cell Types
Publish Date: September 09, 2019 Views: 156 -
24:56GI Tract Organoids: Using Advanced Tissue Models to Interrogate Absorption and Regulation
Publish Date: September 04, 2019 Views: 102 -
33:22CD8+ TILs and Breast Cancer: Identifying New Opportunities for Immunotherapeutic Intervention
Publish Date: August 30, 2019 Views: 28 -
40:54Re-imagining CRISPR Technology for Use with Primary Immune Cells: New Insights for Cell-Based Immunotherapy Research
Publish Date: August 28, 2019 Views: 55 -
12:13Optimization of Normal Human Bronchial Epithelial Cell 3D Cultures for In Vitro Lung Model Studies
Publish Date: August 23, 2019 Views: 163 -
42:10Development, Compatibility, and Applications of mTeSR™ Plus; an Enhanced Medium for the Maintenance of Human Pluripotent Stem Cells (hPSCs)
Publish Date: August 14, 2019 Views: 220 -
55:573D Modeling of Skeletal Muscle for In Vitro Functional Assaying
Publish Date: May 30, 2019 Views: 225 -
57:20Improving Genome Editing with Enhanced CRISPR-Cas Nucleases
Publish Date: May 21, 2019 Views: 221 -
10:16Collaborative Research, Fostering Trust, Advancing Science
Publish Date: May 01, 2019 Views: 203 -
1:51MesenCult™ for Mesenchymal Stromal Cell Derivation, Culture & Differentiation
Publish Date: April 09, 2019 Views: 696 -
0:52AggreWell™: Uniform, size-controlled embryoid bodies and 3D spheroid cultures
Publish Date: April 04, 2019 Views: 2035 -
6:31Drug Screening and Phenotypic Analysis in a Microwell-based 3D Cell Culture System
Publish Date: March 22, 2019 Views: 373 -
51:30Studying Cystic Fibrosis Using Primary Human Nasal Epithelial Cells
Publish Date: March 21, 2019 Views: 7 -
10:08CRISPR-Cas9 Gene Editing of Cerebral Organoids to Model Microcephaly
Publish Date: February 25, 2019 Views: 499 -
16:31Modeling Host-Microbe Interactions Using Human Intestinal Organoids
Publish Date: January 14, 2019 Views: 225 -
29:55Considerations to Streamline the Flow Cytometry Crossmatch (FCXM) Assay
Publish Date: January 02, 2019 Views: 102
