Cell Centrifugation Speed, Time, and Temperature
Optimal centrifugation conditions are required for specific cell types. This guide provides centrifugation speed, time & temperature for various protocols
When centrifuging cells, it’s important to consider the most appropriate centrifuge settings for your cell type and application. Speed, time, and sample temperature are important conditions that should be optimized for your specific protocol. Below is a quick guide for the centrifuge settings we recommend for a range of cell washing, separation, and processing procedures for different cell types, including mononuclear cells, mesenchymal stromal cells, lymphoid and myeloid cells, and neurospheres.
Centrifugation Procedures for Washing Cells
*Room temperature: 15 - 25 °C
Centrifugation Procedures by Cell Separation Method
Density gradient medium for mononuclear cell isolation
(Or: 1200 x g)
(Or: 20 min)
> 24 hrs old: 20 min
> 24 hrs old: 20 min
Centrifugation Procedures for Processing Neurospheres
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A cost-effective alternative to Ficoll-Paque™ that can be used with SepMate™ for the isolation of mononuclear cells from blood.
RosetteSep™ Immunodensity Cell Separation
Isolate human cells directly from whole blood during density gradient centrifugation. Combine with SepMate™ for a faster protocol.
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