Centrifugation Speed and Time for Cells

Optimal centrifugation procedures are required for specific cell types and samples. Below is a quick guide for the centrifugation speed, time, and temperature that we recommend for use with different protocols.

Centrifugation Procedures for Washing Cells

Method
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Regular Cell Wash
300 x g
5 - 10 min
Room temperature*
On
Gentle Cell Wash
100 x g
5 - 6 min
Room temperature
On
Thawed Cell Wash
300 x g
5 - 10 min
2 - 8 °C
On
Platelet Removal Wash
120 x g
10 min
Room temperature
Off


*Room temperature: 15 - 25 °C



Centrifugation Procedures by Cell Separation Method

Method
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Ficoll-Paque™ or Lymphoprep™
Density gradient medium for mononuclear cell isolation
400 x g
(Or: 1200 x g)
30 min
(Or: 20 min)
Room temperature
Off
1200 x g
20 min
Room temperature
Off
RosetteSep™
(for mesenchymal stromal cells)
300 x g
25 min
Room temperature
Off
RosetteSep™ Chimerism Analysis
(for lymphoid cells or myeloid cells)
330 x g
25 min
Room temperature
Off
1200 x g
Fresh sample: 10 min
> 24 hrs old: 20 min
Room temperature
Off
Sepmate™ with RosetteSep™
1200 x g
Fresh sample: 10 min
> 24 hrs old: 20 min
Room temperature
Off


Centrifugation Procedures for Processing Neurospheres

Cell Type
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Neurospheres
90 x g
5 min
Room temperature
On
Dissociated Neurospheres
150 x g
5 min
Room temperature
On



Related Resources

Density gradient centrifugation

The Principles of Using Density Gradient Centrifugation to Isolate Cells

Learn More >

PBMC isolation

How to Isolate PBMCs from Whole Blood by Density Gradient Centrifugation

Watch Video >

View more centrifugation protocols, technical tips, videos, and more >


Related Products

Density gradient medium

Lymphoprep™ Density Gradient Medium

A cost-effective alternative to Ficoll-Paque™ that can be used with SepMate™ for the isolation of mononuclear cells from blood.

View Product >

RosetteSep™ Immunodensity Cell Separation

RosetteSep™ Immunodensity Cell Separation

Isolate human cells directly from whole blood during density gradient centrifugation. Combine with SepMate™ for a faster protocol.

Learn More >

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