Centrifugation Guide

Optimal centrifugation conditions are required for specific cell types and samples. Below is a quick guide for centrifugation speed, time and temperature that we recommend for use with different protocols.

For Washing Cells

Method
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Regular Cell Wash
300 x g
5 - 10 min
Room temperature*
On
Gentle Cell Wash
100 x g
5 - 6 min
Room temperature
On
Thawed Cell Wash
300 x g
5 - 10 min
2 - 8 °C
On
Platelet Removal Wash
120 x g
10 min
Room temperature
Off


*Room temperature: 15 - 25 °C



By Cell Separation Method

Method
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Ficoll-Paque™
Density gradient medium for MNC isolation
400 x g
(Or: 1200 x g)
30 min
(Or: 20 min)
Room temperature
Off
1200 x g
20 min
Room temperature
Off
RosetteSep™
(for mesenchymal stromal cells)
300 x g
25 min
Room temperature
Off
RosetteSep™ Chimerism Analysis
(for lymphoid cells or myeloid cells)
330 x g
25 min
Room temperature
Off
1200 x g
Fresh sample: 10 min
> 24 hrs old: 20 min
Room temperature
Off
Sepmate™ with RosetteSep™
1200 x g
Fresh sample: 10 min
> 24 hrs old: 20 min
Room temperature
Off


For Processing Neurospheres

Cell Type
Centrifugation Speed
Centrifugation Time
Centrifugation Temperature
Brake Setting (On/Off)
Neurospheres
90 x g
5 min
Room temperature
On
Dissociated Neurospheres
150 x g
5 min
Room temperature
On



Related Resource

Choosing a Cell Separation Method that Meets Your Research Needs

Learn more about the cell separation methods outlined above to choose the best method for your application.

Compare Methods >