Centrifugation Guide

Optimal centrifugation conditions are required for specific cell types and samples. Below is a quick guide for centrifugation speed, time and temperature that we recommend for use with different protocols.

Cell type or separation description

Centrifugation speed

Centrifugation time

Centrifugation temperature

Brake

(on / off)

WASHING CELLS

Regular cell wash

300 x g

5 - 10 min

Room Temperature*

On

Gentle cell wash

100 x g

5 - 6 min

Room Temperature

On

Thawed cell wash

300 x g

5 - 10 min

2 - 8°C

On

Platelet removal wash

120 x g

10 min

Room Temperature

Off

CELL SEPARATION

Ficoll**

400 x g
(OR: 1200 x g)

30 min
(OR: 20 min)

Room Temperature

Off

Regular RosetteSep™

1200 x g

20 min

Room Temperature

Off

RosetteSep™ MSC

300 x g

25 min

Room Temperature

Off

RosetteSep™ Chimerism

(Lymphoid and Myeloid)

330 x g

25 min

Room Temperature

Off

Sepmate™

1200 x g

Fresh sample: 10 min
> 24 hrs old: 20 min

Room Temperature

On

Sepmate™ with RosetteSep™

1200 x g

Fresh sample: 10 min
> 24 hrs old: 20 min

Room Temperature

On

OTHER       

Neurospheres

90 x g

5 min

Room Temperature

On

Dissociated Neurospheres

150 x g

5 min

Room Temperature

On

*Room Temperature = 15-25°C

**Ficoll-Paque™ density gradient medium for MNC isolation

 

For further assistance and information, please contact techsupport@stemcell.com.