Know how to characterize your pluripotent stem cells, for confidence in your cultures.
Impact of Genomic Integrity
- When hPSCs Display Hallmarks of Cancer: Hear Dr. Peter Andrews and Dr. Ivana Barbaric from the University of Sheffield discuss the causes and consequences of genetic changes in hPSCs, including acquisition of genetic changes that render them less susceptible to differentiation and/or death. View Now >
- Validation Through Trilineage Differentiation: Advances in protocols for directed differentiation towards the primary germ layers now allow researchers to assess stem cell pluripotency in vitro without a teratoma assay. View Now >
Controlling Culture System & Morphology
- Considerations for Culture pH: In this paper, Wilmes et al. describe the negative effects of extracellular acidification on induced pluripotent stem cells (iPSCs). View Now >
Mastering Pluripotent Stem Cell Quality Characterization
In high quality cultures, certain cell surface and intracellular markers should be homogeneously expressed in nearly all cells.
Due to transient epigenetic memory, using only iPS cells that are fully reprogrammed and characterized is important to avoid aberrations.
Genome Editing of hPSCs
- In this webinar, Dr. Chad Cowan from Harvard University discusses the design and use of TALENs or CRISPR-Cas9 systems with hPSCs. He also covers the applications of modified hPSCs, including disease modeling and gene therapy. View Now >