Chat with an Expert

When to use NeuroFluor™ CDr3 versus NeuroFluor™ NeuO?

Both NeuroFluor™ CDr3 and NeuroFluor™ NeuO are membrane-permeable fluorescent probes for live staining of stage-specific neural cultures. No fixation is required.

The NeuroFluor™ CDr3 (Catalog #01800) should be used to identify primary and pluripotent stem cell-derived neural progenitor cells from different species including but not limited to mouse, rat and human1.

NeuroFluor™ NeuO (Catalog #01801) should be used to identify primary and pluripotent stem cell-derived neurons from different species including but not limited to mouse, rat, human and zebrafish. This probe can be used with in vitro, in vivo and brain slices2.

Figure 1. Infographic demonstrating which NeuroFluor™ probe to use at each stage of neural differentiation.

Table 1. Excitation and Emission for Each NeuroFluor™ Probe

NeuroFluor™ CDr3 and NeuroFluor™ NeuO can be used together to assess stage-specific neural differentiation in cultures. In-house, we have done this using immunocytochemistry.

Table 2. *Filter settings to use with Immunostaining both NeuroFluor™ CDr3 and NeuroFluor™ NeuO

*These are specific filter settings we use with our fluorescent microscope in-house, other filter sets might also be compatible.

For further inquiries about this product, please contact Product and Scientific Support at techsupport@stemcell.com.

References:

NeuroFluor™ CDr3:

  1. Leong C et al. (2013) Neural stem cell isolation from the whole mouse brain using the novel FABP7-binding fluorescent dye, CDr3. Stem Cell Res 11(3): 1314–1322.

NeuroFluor™ NeuO:

  1. Er J et al. (2015) NeuO: A fluorescent chemical probe for live neuron labeling. Angewandte Chemie 54(8): 2442–6.