StemSpan™ Megakaryocyte Expansion Supplement (100X)
Serum-free culture supplement for expansion of human megakaryocytes
Request Pricing
Thank you for your interest in this product. Please provide us with your contact information and your local representative will contact you with a customized quote. Where appropriate, they can also assist you with a(n):
Estimated delivery time for your area
Product sample or exclusive offer
In-lab demonstration
Overview
StemSpan™ Megakaryocyte Expansion Supplement (100X) is intended for use in combination with any of the following StemSpan™ media:
• StemSpan™ SFEM (Catalog #09600)
• StemSpan™ SFEM II (Catalog #09605)
• StemSpan™-XF (Catalog #100-0073)
• StemSpan™-AOF (Catalog #100-0130)
When added to serum-free medium, StemSpan™ Megakaryocyte Expansion Supplement typically promotes the production of hundreds of megakaryocytes per input CD34+ cell in 14-day liquid cultures initiated with CD34+ human CB cells. See data tab for more details.
• Recombinant human interleukin 6 (IL-6)
• Recombinant human interleukin 9 (IL-9)
• Recombinant human thrombopoietin (TPO)
Data Figures

Figure 1. Production of Megakaryocytes by Expansion and Lineage-Specific Differentiation of CD34+ Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing Megakaryocyte Expansion Supplement
Flow cytometry dot plots showing expression of the hematopoietic stem and progenitor cell marker CD34 and megakaryocyte markers CD41a and CD42b (A) before and (B,C) after culture of CD34+ cord blood cells for 14 days in StemSpan™ SFEM containing Megakaryocyte Expansion Supplement. The frequency of CD34+ cells declined from 90% before culture to <3% after 14 days, in parallel with a gradual accumulation of CD41a+CD42b+ megakaryocytes from 80% before and after culture, respectively.
Table 1. Production of Megakaryocytes from CD34+ Human Cord Blood Cells Cultured in StemSpan™ SFEM Containing Megakaryocyte Expansion Supplement

Numbers and percent of CD41a+ cells produced after 14 days of culture of enriched CD34+ cells from 6 independent cord blood (CB) samples. *95% confidence limits, the range within which 95% of the results will typically fall.

Figure 2. Comparison of Megakaryocyte Expansion in Different StemSpan™ Media Containing Megakaryocyte Expansion Supplement
(A) Average numbers and (B) frequencies of CD41+ megakaryocytic cells normalized relative to the values obtained in StemSpan™ SFEM (grey bars) after culturing purified CD34+ cord blood cells (n=6) for 14 days in StemSpan™ SFEM, SFEM II (blue bars) and AOF (orange bars) media containing Megakaryocyte Expansion Supplement. Vertical lines indicate 95% confidence limits, the range within which 95% of results typically fall.
*The numbers of CD41a+ cells were significantly higher in SFEM II (p<0.01, paired t-test, n=6) compared to SFEM and AOF medium.
Note: Data for StemSpan™-AOF shown were generated with the original phenol red-containing version StemSpan™-ACF (Catalog #09855). However internal testing showed that the performance of the new phenol red-free, cGMP-manufactured version, StemSpan™-AOF (Catalog #100-0130) was comparable.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (7)
Publications (11)
Abstract
Abstract
Abstract
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.