STEMscript™ cDNA Synthesis Kits

For reverse transcription of RNA before qPCR or RNA sequencing
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers

For reverse transcription of eukaryotic mRNA before qPCR or RNA sequencing

1 Kit
Catalog # 79003
540 USD
STEMscript™ cDNA Synthesis Kit with Random Primers

For reverse transcription of RNA without poly(A) tails, before qPCR or RNA sequencing

1 Kit
Catalog # 79004
540 USD
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Required Products
  1. Filtered Pipette Tips, 1000 µL
    Corning® Filtered Pipette Tips

    Sterile, polypropylene filtered pipette tips

  2. Total RNA Purification Kit
    Total RNA Purification Kit

    For purification of RNA from cells

Overview

STEMscript™ cDNA Synthesis Kit includes three components that have been optimized to provide efficient synthesis of first-strand cDNA in preparation for qPCR amplification: STEMscript™ Reverse Transcriptase Mix (contains STEMscript™ Reverse Transcriptase purified from recombinant E. coli, and recombinant ribonuclease inhibitor), STEMscript™ Reaction Buffer (contains primers, MgCl₂, and dNTPs), and nuclease-free water. This kit can be used to reverse transcribe total RNA, mRNA, or synthetic transcript RNA, and is required for gene expression analyses such as qPCR and RNA sequencing.

STEMscript™ cDNA Synthesis Kit is available with either oligo(dT) or random primers. Oligo(dT) primers anneal to the poly(A) tails of eukaryotic mRNAs, whereas random primers are used for reverse transcription of RNA without poly(A) tails, such as rRNA, tRNA, degraded RNA, RNA isolated from prokaryotic sources, and RNA with known secondary structures.
Components
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers (Catalog #79003)
• STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
• Nuclease-Free Water, 2 x 1.25 mL
• STEMscript™ Reaction Buffer with Oligo(dT) Primers, 2 x 200 µL
 
STEMscript™ cDNA Synthesis Kit with Random Primers (Catalog #79004)
• STEMscript™ Reverse Transcriptase Mix, 2 x 100 µL
• Nuclease-Free Water, 2 x 1.25 mL
• STEMscript™ Reaction Buffer with Random Primers, 2 x 200 µL
Species
Human, Mouse, Rat
Brand
STEMscript

Scientific Resources

Product Documentation

Document Type Product Name Catalog # Lot # Language
Document Type
Product Information Sheet
Product Name
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
Catalog #
79003
Lot #
All
Language
English
Document Type
Product Information Sheet
Product Name
STEMscript™ cDNA Synthesis Kit with Random Primers
Catalog #
79004
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
Catalog #
79003
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
Catalog #
79003
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMscript™ cDNA Synthesis Kit with Oligo(dT) Primers
Catalog #
79003
Lot #
All
Language
English
Document Type
Safety Data Sheet 1
Product Name
STEMscript™ cDNA Synthesis Kit with Random Primers
Catalog #
79004
Lot #
All
Language
English
Document Type
Safety Data Sheet 2
Product Name
STEMscript™ cDNA Synthesis Kit with Random Primers
Catalog #
79004
Lot #
All
Language
English
Document Type
Safety Data Sheet 3
Product Name
STEMscript™ cDNA Synthesis Kit with Random Primers
Catalog #
79004
Lot #
All
Language
English

Educational Materials (1)

Brochure
qPCR Arrays for Cell Characterization

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