STEMdiff™ Neural Induction Medium Versus STEMdiff™ SMADi Neural Induction Kit

STEMdiff™ Neural Induction Medium (NIM, Catalog # 05835) is a serum-free medium for the neural induction of human pluripotent stem cells (hPSCs), including human embryonic stem (ES) cells and induced pluripotent stem (iPS) cells. The STEMdiff™ SMADi Neural Induction Kit (Catalog # 08581) combines STEMdiff™ NIM with the STEMdiff™ SMADi Neural Induction Supplement, which directs differentiation by inhibiting TGF-β/BMP-dependent SMAD signaling. Both media enable highly efficient generation of neural progenitor cells (NPCs) using either embryoid body (EB)- or monolayer culture-based protocols. The cultures generated will be enriched for central nervous system (CNS)-type NPCs, which express SOX1, Nestin, and PAX6. Below, we highlight advantages of the STEMdiff™ SMADi Neural Induction Kit as it utilizes the same mechanisms as Lorenz Studer’s Dual SMAD inhibition approach1:


STEMdiff™ Neural Induction Medium (Catalog # 05835)
STEMdiff™ SMADi Neural Induction Kit (Catalog # 08581)
Serum-free?
Yes
Yes
Compatibility with hPSCs maintained in mTeSR™1 or TeSR™-E8™
EB Protocol: mTeSR™1 and TeSR™-E8™
Monolayer culture protocol: mTeSR™1
EB and monolayer culture protocols: mTeSR™1 and TeSR™-E8™
Quality of starting cells
Ensure that high-quality hPSCs are used.
Highly efficient neural induction of hard-to-differentiate lines.
Downstream protocol
Must use EB protocol if performing downstream differentiation with:

  • STEMdiff™ Neuron Differentiation Kit (Catalog # 08500) and STEMdiff™ Maturation Kit (Catalog # 08510)
  • STEMdiff™ Dopaminergic Neuron Differentiation Kit (Catalog # 08520) and STEMdiff™ Dopaminergic Neuron Maturation Kit (Catalog # 08530)
  • STEMdiff™ Astrocyte Differentiation Kit (Catalog # 08540) and STEMdiff™ Astrocyte Maturation Kit (Catalog # 08550)
Use either the EB or monolayer protocol for downstream differentiation with:

  • STEMdiff™ Neuron Differentiation Kit (Catalog # 08500) and STEMdiff™ Maturation Kit (Catalog # 08510)
  • STEMdiff™ Dopaminergic Neuron Differentiation Kit (Catalog # 08520) and STEMdiff™ Dopaminergic Neuron Maturation Kit (Catalog # 08530)
  • STEMdiff™ Astrocyte Differentiation Kit (Catalog # 08540) and STEMdiff™ Astrocyte Maturation Kit (Catalog # 08550)
Compatibility with RSeT™- and RSeT™ Feeder Free-induced naïve-like hPSCs
Naïve-like hPSCs require one priming passage in mTeSR™1.
Naïve-like hPSCs can be directly differentiated using the STEMdiff™ SMADi Neural Induction Kit.*

*(Naïve-like hPSCs generated with NaïveCult™ will require re-priming for 2-4 passages).

For performance data of the STEMdiff™ SMADi Neural Induction Kit, refer to the data and publications sections of the product page.

For further inquiries about this product, please contact Product and Scientific Support at techsupport@stemcell.com.

References

  1. Chambers SM et al. (2009) Highly efficient neural conversion of human ES and iPS cells by dual inhibition of SMAD signaling. Nat Biotechnol 27(3): 275-80.