ThawSTAR® CB Automated Thawing System
Automated high-volume cell thawing system for consistent performance
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ThawSTAR® CB Barrier BagsSingle-use barrier bags for proper positioning of the cryobags during thawing
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ThawSTAR® CB IOPQ KitInstallation, operational, and performance qualification documentation and accessories to facilitate functional testing of ThawSTAR® CB
Overview
ThawSTAR® CB is compatible with most major cryobag manufacturers and sizes, including STEMCELL’s Frozen Human Peripheral Blood Leukopaks (Catalog #200-0130/200-0131/200-0132/200-0470).
Data Figures
Figure 1. Frozen Human Peripheral Blood Leukopaks Thawed Using the ThawSTAR® CB Automated Thawing System Show High Recovery and Viability for Pre-wash Assessment
Frozen Human Peripheral Blood Leukopaks (Quarter Size; Catalog #200-0132) were retrieved from liquid nitrogen and thawed using either ThawSTAR® CB Automated Thawing System with appropriately selected thawing settings or a water bath. When thawed using the ThawSTAR® CB Automated Thawing System or a water bath, (A) the mean live cell recovery was 4.42 x 10⁹ vs. 3.58 x 10⁹ cells for Donor 1, 4.53 x 10⁹ vs. 4.95 x 10⁹ cells for Donor 2, 3.94 x 10⁹ vs. 4.52 x 10⁹ cells for Donor 3, respectively. (B) The mean viability of automatic vs. water bath-thawed cells was 93.3% vs. 95.4% for Donor 1, 98.9% vs. 98.3% for Donor 2, and 98.2% vs. 98.5% for Donor 3, respectively. The cells were from 3 different donors and were tested in triplicates. Cell recovery and viability was assessed using a NucleoView™ counter.
Figure 2. Frozen CD4+ Cells Thawed Using the ThawSTAR® CB Automated Thawing System Show High Recovery and Viability for Pre-wash Assessment
CD4+ cells cryopreserved in CryoStor® CS10 (Catalog #07930) were retrieved from liquid nitrogen and thawed using either ThawSTAR® CB Automated Thawing System with appropriately selected thawing settings or a water bath. When thawed using the ThawSTAR® CB Automated Thawing System or a water bath, (A) the mean live cell recovery was 1.27 x 10⁹ vs. 1.19 x 10⁹ cells for Donor 1, 1.03 x 10⁹ vs. 1.16 x 10⁹ cells for Donor 2, respectively and (B) the mean viability was 92.0% vs. 93.3% for Donor 1, 95.2% vs. 97.4% for Donor 2, respectively. The cells were from 2 different donors and were tested in triplicates. Cell recovery and viability were assessed using a NucleoView™ counter.
Protocols and Documentation
Find supporting information and directions for use in the Product Information Sheet or explore additional protocols below.
Applications
This product is designed for use in the following research area(s) as part of the highlighted workflow stage(s). Explore these workflows to learn more about the other products we offer to support each research area.
Resources and Publications
Educational Materials (8)
Related Products
Quality Statement:
PRODUCTS ARE FOR RESEARCH USE ONLY AND NOT INTENDED FOR HUMAN OR ANIMAL DIAGNOSTIC OR THERAPEUTIC USES UNLESS OTHERWISE STATED. FOR ADDITIONAL INFORMATION ON QUALITY AT STEMCELL, REFER TO WWW.STEMCELL.COM/COMPLIANCE.
